中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1225-1228.doi: 10.3969/j.issn.1673-8225.2012.07.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

疲劳型亚健康大鼠模型的构建*★

李玉萍1，黄少慧1，靳  文2   

  1. 1南方医科大学中医药学院(南方医科大学中西医结合医院)，广东省广州市  510515；2广州市红十字会医院心内科，广东省广州市  510220lee020@21cn.com
  • 收稿日期:2011-12-01 修回日期:2011-12-21 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 靳文，博士，主治医师。广州市红十字会医院心内科，广东省广州市 510220 48457742@qq.com
  • 作者简介:李玉萍★，女，1975年生，湖北省大悟县人，汉族，2006年南方医科大学毕业，硕士，主要从事亚健康的临床与实验研究。 lee020@sina.com
  • 基金资助:

    广州市科技三项基金资助课题(编号，2006Z3-E5051-3)，课题名称：维康Ⅰ号软胶囊防治肝肾阴虚型亚健康的开发研究。

Construction of a sub-health fatigue rat model

Li Yu-ping1, Huang Shao-hui1, Jin Wen2   

  1. 1College of Traditional Chinese Medicine, Southern Medical University (Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University), Guangzhou  510515, Guangdong Province, China; 2Department of Cardiology, Guangzhou Red Cross Hospital, Guangzhou  510220, Guangdong Province, China
  • Received:2011-12-01 Revised:2011-12-21 Online:2012-02-12 Published:2012-02-12
  • Contact: Jin Wen, Doctor, Attending physician, Department of Cardiology, Guangzhou Red Cross Hospital, Guangzhou 510220, Guangdong Province, China 48457742@qq.com
  • About author:Li Yu-ping★, Master, College of Traditional Chinese Medicine, Southern Medical University (Hospital of Integrated Traditional Chinese and Western Medicine, Southern Medical University), Guangzhou 510515, Guangdong Province, China lee020@sina.com
  • Supported by:

    Guangzhou Municipal Science and Technology Three Projects Fund, No. 2006Z3-E5051-3*

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摘要：

背景：有研究用复合因素构建了亚健康及慢性疲劳大鼠模型，但疲劳型亚健康大鼠模型的构建较罕见。
目的：采用力竭游泳复合睡眠剥夺构建疲劳型亚健康大鼠模型，探讨最佳的疲劳型亚健康大鼠模型制备方法。
方法：将大鼠负重5%力竭游泳并睡眠剥夺20 h建立疲劳型亚健康模型，记录力竭游泳时间，各组实验结束禁食1 d，行血液生化检查。
结果与结论：建模后4，5 d大鼠末次力竭游泳时间显著减少(P < 0.05)。建模后5 d，大鼠血α-羟丁酸脱氢酶、尿素氮、血肌酸激酶升高(P < 0.05)、总胆固醇降低，门冬氨酸氨基转移酶、丙氨酸氨基转移酶、血清肌酐、三酰甘油水平均降低        (P < 0.05)。结果证实，负重5%的力竭游泳复合20 h睡眠剥夺持续4 d即可成功制备疲劳型亚健康大鼠模型。

关键词: 疲劳型亚健康, 力竭游泳, 睡眠剥夺, 血液生化, 组织工程

Abstract:

BACKGROUND: Previously, sub-health and chronic fatigue model is constructed with complex physical factors, but sub-health fatigue rat model is rarely report.
OBJECTIVE: To observe the sub-health fatigue rat models constructed by sleep deprivation combined with exhaustive weight-loading swimming exercise.
METHODS: Thirty-two male rats were randomly divided into four groups by weight. Control group, 1-day model, 4-day model and 5-day model group. Rats in the model group were used to establish the model of sub-health fatigue by being deprived of sleep for 20 hours, and then the rats were required to conduct exhaustive swimming exercise with loading 5% of rat body weight. The exhaustive swimming time was recorded. Each group was preformed with fasting for one day in the end of the experiment, and toke the blood biochemistry.
RESULTS AND CONCLUSION: Compared the last exhaustive swimming time with the first, the exhaustive swimming time of 4-day model and 5-day model group was shorter significantly (P < 0.05). The levels of blood Alpha-hydroxybutyrate dehydrogenase and blood urea nitrogen in 5-day group was increased compared with the rest groups (P < 0.05). The levels of blood cholesterol aspartate aminotransferase, alanine aminotransferase, creatinine and triglyceride were decreased (P < 0.05). Exhaustive swimming exercise with loading 5% of rat body weight and sleep deprivation for 20 hours might establish a rat model of sub-health fatigue.

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