中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (53): 9996-9998.doi: 10.3969/j.issn.1673-8225.2011.53.028

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

猪不停跳心脏离体模型的建立

郑  民，宋剑非，杨  峰，张文斌   

  1. 桂林医学院附属医院胸心外科，广西壮族自治区桂林市 541001
  • 收稿日期:2011-09-14 修回日期:2011-09-20 出版日期:2011-12-31 发布日期:2011-12-31
  • 作者简介:郑民★，男，1971年生，广东省中山市人，汉族，2005年上海第二医科大学毕业，硕士，副主任医师，主要从事心肺移植，血管新生方面的研究。 lordzm@163.com
  • 基金资助:

    广西科学基金项目(桂科基0236068)，供体心脏不停跳和停跳保存方法对心肌保护的对比研究。

Establishment of a porcine beating heart model in vitro

Zheng Min, Song Jian-fei, Yang Feng, Zhang Wen-bin   

  1. Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-14 Revised:2011-09-20 Online:2011-12-31 Published:2011-12-31
  • About author:Zheng Min★, Master, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China lordzm@163.com
  • Supported by:

    Science Foundation of Guangxi Zhuang Autonomous Region, No. 0236068*

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摘要：

背景：保持供心离体跳动，可以减少供心的缺血时间，延长供心的离体保存时间。
目的：建立稳定简便的猪不停跳心脏离体模型。
方法：12只健康家猪随机等分为不停跳组和冷晶体灌注组。不停跳组经升主动脉建立冠状动脉灌注，经右心房进右心室建立静脉引流，经左心耳放置软管入左心室引流，建立猪的不停跳心脏离体模型。冷晶体灌注组按常规心脏移植实行心脏离体保护。
结果与结论：不停跳组共施行6例猪心脏离体手术，术后2，4 h离体心脏不停跳成功率为100%(6/6)，6 h离体心脏不停跳成功率为83%(5/6)。与对照组相比，不停跳组丙二醛水平降低，超氧化物歧化酶活性升高(P < 0.05)，心肌超微结构保持良好。说明猪的不停跳心脏离体模型成功率高，是研究离体不停跳心脏仪器的稳定可靠动物模型。

关键词: 心脏不停跳, 离体, 猪, 心肌保护, 心脏移植

Abstract:

BACKGROUND: Keeping beating of the donor heart can reduce its ischemia time and prolong its preservation time in vitro.
OBJECTIVE: To establish a simple and stable model of porcine beating heart in vitro.
METHODS: Twelve healthy pigs were randomly divided into beating group and cold crystalloid perfusion group. In the beating group, coronary perfusion was established through the ascending aorta, venous drainage was established through right atrium into the right ventricle, the hose was placed into the left ventricle through the left atrial appendage for drainage, and then the model of porcine beating heart in vitro was established. Heart protection in vitro was performed in the cold crystalloid perfusion group after normal heart transplantation.
RESULTS AND CONCLUSION: Surgery in vitro of porcine heart was successfully performed on six pigs of the beating group. The success rate of beating heart was 100% after 2 and 4 hours of surgery, and was 83% at 6 hours. The malondialdehyde levels were decreased, superoxide dismutase activities were increased (P < 0.05), and the myocardial ultrastructure was well kept in the beating group compared with the cold crystalloid perfusion group. The porcine beating heart model has a high success rate in modeling, and it is an ideal animal model for studying beating heart in vitro.

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