中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8797-8800.doi: 10.3969/j.issn.1673-8225.2011.47.014

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

内皮祖细胞在膀胱细胞外基质上的生长及分化

萧安格，赵凯亮，杨嗣星，李永伟，廖文彪，孟令超   

  1. 武汉大学人民医院泌尿外科，湖北省武汉市  430060
  • 收稿日期:2011-04-16 修回日期:2011-05-19 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 杨嗣星，教授，博士生导师，武汉大学人民医院泌尿外科，湖北省武汉市 430060 sxyang2004@ 163.com
  • 作者简介:萧安格★，男，1973年生，台湾人，汉族，武汉大学第一临床医学院在读硕士，主要从事泌尿组织工程学研究。 chensi271@ yahoo.com.cn 并列第一作者：赵凯亮★，男，1983年生，山西省晋城市人，汉族，武汉大学第一临床医学院在读硕士，主要从事泌尿组织工程学研究。 wenlu1985@yahoo.cn
  • 基金资助:

    国家自然科学基金项目(30872594)。

Growth and differentiation of endothelial progenitor cells on the bladder extracellular matrix

Xiao An-ge, Zhao Kai-liang, Yang Si-xing, Li Yong-wei, Liao Wen-biao, Meng Ling-chao   

  1. Department of Urinary Surgery, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
  • Received:2011-04-16 Revised:2011-05-19 Online:2011-11-19 Published:2011-11-19
  • Contact: Yang Si-xing, Professor, Doctoral supervisor, Department of Urinary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
  • About author:Xiao An-ge★, Studying for master’s degree, Department of Urinary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China chensi271@ yahoo.com.cn Zhao Kai-liang, Studying for master’s degree, Department of Urinary Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China wenlu1985@ yahoo.cn Xiao An-ge and Zhao Kai-liang contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No. 30872594*

PDF

265

被引次数

3

摘要：

背景：传统的组织工程膀胱支架材料本身无血管结构，植入体内后面临血管化不足的问题。
目的：观察内皮祖细胞与膀胱细胞外基质的生物相容性。
方法：分离培养兔内皮祖细胞，种植于兔膀胱细胞外基质上与其复合培养。将复合生长材料植入兔背部皮下检测其组织相容性。
结果与结论：兔内皮祖细胞能够在膀胱细胞外基质表面正常黏附、生长、增殖，细胞形态良好。内皮祖细胞-膀胱细胞外基质植入兔体内后1周时，可见材料周围炎症反应明显，粘连严重，出血较多；苏木精-伊红染色可见组织中较多炎性细胞浸润，胶原及弹性纤维排列松散。植入后8周时可见材料已降解成碎细丝状，与周围组织融合生长在一起，但质地略脆，易出血；苏木精-伊红染色可见组织中已无明显炎症细胞浸润反应，胶原及弹性纤维排列紧密并且有新生血管长入其中。结果表明内皮祖细胞与膀胱细胞外基质具有良好的相容性，复合培养物与体内组织具有良好的相容性。

关键词: 膀胱细胞外基质, 内皮祖细胞, 生物相容性, 组织工程, 支架

Abstract:

BACKGROUND: Traditional bladder tissue engineering scaffold materials have no vascular structures, and insufficient vascularization is the problem after implantation in vivo.
OBJECTIVE: To evaluate the biocompatibility of bladder extracellular matrix with endothelial progenitor cells of rabbits.
METHODS: Rabbit’s endothelial progenitor cells were isolated and cultured. The endothelial progenitor cells combined with the bladder extracellular matrix were cultured and seeded in vitro. The histocompatibility of the complex was evaluated after implantation into the rabbit back.
RESULTS AND CONCLUSION: Endothelial progenitor cells were able to adhere, grow, and proliferate on the bladder extracellular matrix surface, showing a good cell shape. At 1 week after the endothelial progenitor cells combined with the bladder extracellular matrix were implanted in rabbits, the distinctive inflammatory reaction around the material could be seen, with severe adhesion and mass bleeding. The hematoxylin-eosin staining showed many inflammatory cells infiltrating tissues, collagen and loose arrangement of elastic fibers. After 8 weeks, the implanted materials had been degraded to be broken filaments, fused with the surrounding tissue, with crisp texture and easy bleeding; the hematoxylin-eosin staining showed no obvious tissue infiltration of inflammatory cells in response, closely arranged collagen and elastic fibers and neovasculature could be seen in the materials. The results indicate that the endothelial progenitor cells have a good compatibility with the bladder extracellular matrix, and the complex has a good compatibility with body tissues.

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