中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8639-8642.doi: 10.3969/j.issn.1673-8225.2011.46.021

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

早期应用生长反应因子1小干扰RNA可抑制烟草烟雾刺激大鼠主动脉平滑肌细胞白细胞介素1β mRNA的表达

刘雪莲1，刘闺男1，郭洪涛1, 2   

  1. 1中国医科大学附属第一医院心血管内科，辽宁省沈阳市110001
    2本溪钢铁(集团)有限责任公司总医院心内科，辽宁省本溪市 117000
  • 收稿日期:2011-06-05 修回日期:2011-09-10 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 刘闺男，硕士，教授，博士生导师，中国医科大学附属第一医院心血管内科，辽宁省沈阳市110001 guinanliu@hotmail.com
  • 作者简介:刘雪莲★，女，1983年生，河北省人，汉族， 2011年中国医科大学毕业，硕士，主要从事冠心病防治及血管再狭窄的机制与临床研究。 snowlau19831112@yahoo.cn
  • 基金资助:

    课题受国家自然科学基金项目(30871074)资助。

Early application of growth response-1 small interfering RNA can inhibit the expression of interleukin-1 beat mRNA induced by cigarette smoke in rat aortic vascular smooth muscle cells

Liu Xue-lian1, Liu Gui-nan1, Guo Hong-tao1, 2   

  1. 1Department of Cardiology, First Hospital of China Medical University, Shenyang  110001, Liaoning Province, China
    2Department of Cardiology, General Hospital of Benxi Steel Group Corporation, Benxi 117000, Liaoning Province, China
  • Received:2011-06-05 Revised:2011-09-10 Online:2011-11-12 Published:2011-11-12
  • Contact: Liu Gui-nan, Master, Professor, Doctoral supervisor, Department of Cardiology, First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China guinanliu@hotmail.com
  • About author:Liu Xue-lian★, Master, Department of Cardiology, First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China snowlau19831112@yahoo.cn
  • Supported by:

    National Natural Science Foundation of China, No. 30871074*

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摘要：

背景：研究表明，在动脉粥样硬化的血管壁细胞中白细胞介素1β表达水平升高。
目的：观察早期生长反应因子1的小干扰RNA对烟草烟雾提取物刺激大鼠主动脉平滑肌细胞中白细胞介素1β mRNA表达的影响。
方法：体外培养大鼠主动脉平滑肌细胞，用不同体积分数(0%，5%，10%，20%和40%)烟草烟雾提取物刺激细胞，筛选最佳体积分数烟草烟雾提取物干预细胞0，8，16和24 h，以确定最佳干预时间，最后用生长反应因子1小干扰RNA以沉默细胞中生长反应因子1的表达。 
结果与结论：RT-PCR结果显示，烟草烟雾提取物可诱导大鼠主动脉平滑肌细胞白细胞介素1β mRNA的表达，且有时间和浓度依赖性，烟草烟雾提取物最佳干预时间为8 h，最佳干预体积分数为10%。将生长反应因子1小干扰RNA转染10%烟草烟雾提取物刺激的细胞后8 h，白细胞介素1β mRNA表达明显减少(P < 0.05)。证实早期应用生长反应因子1小干扰RNA可以抑制烟草烟雾提取物诱导的大鼠主动脉平滑肌细胞中白细胞介素1β mRNA的表达。

关键词: 血管平滑肌细胞, 白细胞介素1&beta, 早期生长反应因子1, 小干扰RNA, 组织工程

Abstract:

BACKGROUND: Researches show that the expression of interleukin-1β is elevated in atherosclerosis vascular cells.
OBJECTIVE: To investigate the effect of early growth response-1 small interfering RNA on the expression of interleukin-1β mRNA induced by cigarette smoke extract in rat aortic vascular smooth muscle cells.
METHODS: Rat aortic vascular smooth muscle cells were cultured in vitro, then treated with cigarette smoke extracts of different volume fractions (0%, 5%, 10%, 20% and 40%) to screen for the optimal volume fraction. On that basis, the cells were treated for 0, 8, 16 and 24 hours to determine the optimal intervention time. In the end, the expression of growth response-1 was shut down by growth response-1 interfering RNA.
RESULTS AND CONCLUSION: According to RT-PCR, cigarette smoke extract was able to induce interleukin-1β mRNA expression in a dose-dependent and time-dependent manner in rat aortic vascular smooth muscle cells. The optimal intervention time and volume fraction of cigarette smoke extract were 8 hours and 10%. Results presented that cells transfected with small interfering RNA targeting growth response-1 for 8 hours showed a significant reduction in interleukin-1β mRNA expression (P < 0.05), in response to 10% cigarette smoke extract treatment. It proves that early growth response-1 small interfering RNA can inhibit the expression of interleukin-1β mRNA induced by cigarette smoke in rat aortic vascular smooth muscle cells.

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