中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8630-8634.doi: 10.3969/j.issn.1673-8225.2011.46.019

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

心肌缺血损伤小型猪模型内关穴位埋针后血管新生及成纤维细胞生长因子mRNA和蛋白的表达

杨孝芳1，崔  瑾1，刘小雨2，徐  照3，张小珊1，冯  麟1，王兴桂1，钱  宁4   

  1. 贵阳中医学院，1针灸推拿系，3基础部功能形态实验室，4动物研究所，贵州省贵阳市  550002；2同济大学附属第十人民医院中医科，上海市  200072
  • 收稿日期:2011-05-19 修回日期:2011-10-10 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 崔瑾，博士，教授，贵阳中医学院，针灸推拿系，贵州省贵阳市 550002 cj1108@21cn.com
  • 作者简介:杨孝芳☆，女，1975年生，贵州省大方人，蒙古族，博士，副教授，硕士生导师，主要从事针灸治疗心脑血管疾病临床和实验研究。 yangxiaofang210@163.com
  • 基金资助:

    贵州省科学技术基金资助项目(黔科合J[2007]2109号)，课题名称：内关穴埋针对小型猪心肌缺血损伤修复信号传导的研究。

Angiogenesis and expression of fibroblastic growth factor mRNA and protein after needle-embedding therapy of Neiguan in miniature pigs with myocardial ischemia injury

Yang Xiao-fang1, Cui Jin1, Liu Xiao-yu2, Xu Zhao3, Zhang Xiao-shan1, Feng Lin1, Wang Xing-gui1, Qian Ning4   

  1. 1Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China; 2Department of Traditional Chinese Medicine, Tenth People’s Hospital of Tongji University, Shanghai  200072, China; 3Laboratory of Morphology and Function, School of Basic Medical Science, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China; 4Institute of Zoology, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China
  • Received:2011-05-19 Revised:2011-10-10 Online:2011-11-12 Published:2011-11-12
  • Contact: Cui Jin, Doctor, Professor, Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China cj1108@21cn.com
  • About author:Yang Xiao-fang☆, Doctor, Associate professor, Master’s supervisor, Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China yangxiaofang210@163.com
  • Supported by:

    the Guizhou Committee of Science and Technology, No. J [2007]2109*

PDF

341

被引次数

26

摘要：

背景：血管生成及成纤维细胞生长因子与心肌缺血损伤后修复关系密切，针刺内关防治心肌缺血损伤的机制是否与此有关尚不清楚。
目的：观察内关穴位埋针对心肌缺血损伤小型猪血管新生及成纤维细胞生长因子基因和蛋白表达的影响。
方法：将32只小型猪随机分为4组，采用左冠状动脉前降支结扎法建立心肌缺血模型，假手术组穿线但不结扎，内关组和膈俞组分别在造模的基础上进行内关、膈俞穴埋针治疗，模型组和假手术组不进行任何干预。
结果与结论：免疫组织化学染色显示小型猪经冠脉结扎后心肌毛细血管密度降低(P < 0.01)，内关、膈俞穴埋针治疗7 d，损伤心肌组织毛细血管密度增加(P < 0.05或P < 0.01)，内关组优于膈俞组(P < 0.05)；Real time PCR和Western blot检测显示小型猪经冠脉结扎后心肌组织成纤维细胞生长因子mRNA和蛋白表达量显著增高(P < 0.05或P < 0.01)，内关和膈俞穴位埋针治疗均可上调成纤维细胞生长因子mRNA和蛋白表达量，以内关埋针效果最明显(P < 0.05)。揭示内关、膈俞穴位埋针均可通过上调成纤维细胞生长因子mRNA和蛋白的表达，增加心肌毛细血管密度，改善缺血心肌的损伤，且内关优于膈俞。

关键词: 内关, 膈俞, 毛细血管密度, 成纤维细胞生长因子, 心肌缺血, 小型猪, 埋针

Abstract:

BACKGROUND: Angiogenesis and fibroblast growth factors are closely related to the repair of myocardial ischemia injury. Myocardial ischemia injury can be prevented and cured by acupuncturing Neiguan. It is unclear that whether the mechanism behind this is related to the angiogenesis and fibroblast growth factors.
OBJECTIVE: To explore the effect of needle-embedding therapy of Neiguan on the angiogenesis and expression of fibroblast growth factor gene and protein in miniature pigs after myocardial ischemia injury.
METHODS: A number of 32 miniature pigs were randomly divided into 4 groups. The myocardial ischemia injury model was established by ligating the left anterior descending coronary artery. The left anterior descending coronary artery of the sham-operated pigs was only threaded without ligation. Pigs in Neiguan group and Geshu group were treated with needle-embedding therapy of Neiguan and Geshu respectively after establishing the myocardial ischemia injury model. Model group and sham-operated group received no treatment.
RESULTS AND CONCLUSION: According to immunohistochemical staining, the capillary density of cardiac muscle in miniature pigs was lowered after coronary artery ligation (P < 0.05). The capillary density of cardiac muscle increased on the 7th day after needle-embedding therapy of Neiguan and Geshu (P < 0.05 or P < 0.01). Neiguan group preceded Geshu group (P < 0.05). According to real time PCR and western blot, the fibroblast growth factor mRNA and protein expression of myocardial tissue in miniature pigs were significantly improved after coronary artery ligation (P < 0.05 or P < 0.01). The fibroblast growth factor mRNA and protein expression of Neiguan group and Geshu group improved, and the effect of needle-embedding therapy of Neiguan was the most evident (P < 0.05). It reveals that needle-embedding therapy of Neiguan and Geshu can increase the fibroblast growth factor mRNA and protein expression of myocardial tissue, leading to an increase of cardiac muscle capillary density, therefore ameliorate the myocardial ischemia. And the needle-embedding therapy of Neiguan is better than that of Geshu.

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