中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8621-8624.doi: 10.3969/j.issn.1673-8225.2011.46.017

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

胆管癌细胞体外三维培养模型建立与二维培养体系中生长代谢的比较

李  磊1，周伟佳1，王健东2，叶朝阳1，周  燕1，谭文松1   

  1. 1华东理工大学生物反应器工程国家重点实验室，上海市 200237
    2上海交通大学附属新华医院，上海市 200092
  • 收稿日期:2011-06-10 修回日期:2011-09-20 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 周燕，硕士，副教授，华东理工大学生物反应器工程国家重点实验室，上海市 200237 zhouyan@ecust.edu.cn
  • 作者简介:李磊★，男，1985年生，浙江省绍兴市人，汉族， 2011华东理工大学毕业，硕士，主要从事细胞三维培养及干细胞定向诱导分化研究。 lileifalcon@163.com
  • 基金资助:

    课题受上海市自然科学基金资助项目(编号：10ZR1407100)和国家重点实验室专项经费资助项目(编号：2060204)资助。

Comparison of growth and metabolism of cholangiocarcinoma cells in two-dimensional and three-dimensional culture systems

Li Lei1, Zhou Wei-jia1, Wang Jian-dong2, Ye Zhao-yang1, Zhou Yan1, Tan Wen-song1   

  1. 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai  200237, China
    2Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Received:2011-06-10 Revised:2011-09-20 Online:2011-11-12 Published:2011-11-12
  • Contact: Zhou Yan, Master, Associate professor, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China zhouyan@ecust.edu.cn
  • About author:Li Lei★, Master, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China lileifalcon@163.com
  • Supported by:

    the Natural Science Foundation of Shanghai, No. 10ZR1407100*; the National Foundation of Key Laboratory, No. 2060204*

PDF

341

被引次数

4

摘要：

背景：二维细胞培养是目前体外研究恶性肿瘤和检测抗肿瘤药物的常用方法，但存在局限性。
目的：建立胆管癌细胞体外三维培养模型，比较在二维和三维培养体系中胆管癌细胞生长及代谢的差异。
方法：将胆管癌细胞株QBC939培养于二维培养体系及Cytodex-3或Cytopore-2的三维培养体系中，观察细胞形态和数量，测定培养上清液中葡萄糖、乳酸浓度，计算细胞生长代谢动力学参数。
结果与结论：与二维和Cytodex-3三维培养体系相比，由Cytopore-2构成的三维培养体系中细胞在微载体上贴壁率较高，分布均匀，所达到的细胞密度最高，且细胞的葡萄糖比消耗速率和乳酸比生成速率明显低于二维和Cytodex-3三维培养系统(P < 0.05)。结果证实，采用Cytodex-3或Cytopore-2微载体培养体系均可建立胆管癌细胞的三维培养模型，且Cytopore-2三维培养体系优于二维和Cytodex-3三维培养体系。

关键词: 胆管癌细胞, 微载体, 三维培养模型, 葡萄糖, 乳酸, 组织工程

Abstract:

BACKGROUND: Two-dimensional (2D) culture model is a common tool for tumor research and drug screening in vitro; however, there are some limitations.
OBJECTIVE: To establish a three-dimensional (3D) culture model of cholangiocarcinoma cells and to compare the difference in cell growth and metabolism between 2D and 3D culture systems.
METHODS: Cholangiocarcinoma cell line QBC939 was cultured in 2D and Cytodex-3 or Cytopore-2 microcarrier-based 3D culture systems. Cell morphology was observed under inverted light microscopy. Total cell number, glucose and lactate concentrations were determined, and the kinetic constants of cell growth and metabolism were calculated.
RESULTS AND CONCLUSION: In comparison with 2D and Cytodex-3-based 3D culture systems, Cytopore-2-based 3D culture system achieved higher cell adhesion rate, higher cell density, and lower specific glucose consumption and specific lactate production rates (P < 0.05). In addition, cells were uniformly distributed in Cytopore-2 microcarriers. Therefore, Cytodex-3 or Cytopore-2 microcarriers can be used to establish cholangiocarcinoma 3D culture model, and Cytopore-2 3D culture system is better than Cytodex-3 system.

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