中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7071-7074.doi: 10.3969/j.issn.1673-8225.2011.38.010

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

自制小牛脱蛋白松质骨与兔皮质骨来源成骨细胞的生物相容性

孟加榕1，陈长青2，张善涛2，练克俭2，黄立羡2，丁真奇2，尹小峰2   

  1. 1厦门大学附属东南医院病理科，福建省漳州市 363000
    2厦门大学附属东南医院骨科医院(解放军第175医院全军创伤骨科中心)，福建省漳州市 363000
  • 收稿日期:2011-03-14 修回日期:2011-04-13 出版日期:2011-09-17 发布日期:2011-09-17
  • 通讯作者: 陈长青，博士，副主任医师，厦门大学附属东南医院骨科医院，福建省漳州市 363000 chencq1991@ hotmail.com
  • 作者简介:孟加榕，男，1971年生，福建省漳州市人，汉族，1994年福建医科大学毕业，主治医师，主要从事组织工程骨实验和病理学研究。
  • 基金资助:

    福建省青年人才基金项目(2006F3146)；南京军区“十一五”重点课题基金项目(06Z29)。

Biocompatibility of self-made calf deproteinized cancellous bone with rabbit cortical bone-derived osteoblasts

Meng Jia-rong1, Chen Chang-qing2, Zhang Shan-tao2, Lian Ke-jian2, Huang Li-xian2, Ding Zhen-qi2, Yin Xiao-feng2   

  1. 1Department of Pathology, Southeast Hospital of Xiamen University, Zhangzhou  363000, Fujian Province, China
    2Department of Orthopedics, Southeast Hospital of Xiamen University (Center of Orthopedics and Traumatology of PLA, the 175 Hospital of Chinese PLA), Zhangzhou  363000, Fujian Province, China
  • Received:2011-03-14 Revised:2011-04-13 Online:2011-09-17 Published:2011-09-17
  • Contact: Chen Chang-qing, Doctor, Associate chief physician, Department of Orthopedics, Southeast Hospital of Xiamen University (Center of Orthopedics and Traumatology of PLA, the 175 Hospital of Chinese PLA), Zhangzhou 363000, Fujian Province, China chencq1991@ hotmail.com
  • About author:Meng Jia-rong, Attending physician, Department of Pathology, Southeast Hospital of Xiamen University, Zhangzhou 363000, Fujian Province, China
  • Supported by:

    the Youth Talent Fund of Fujian Province, No. 2006F3146*; the Key Subject Fund of Nanjing Military Command during the Eleventh Five-year Plan, No. 06Z29*

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被引次数

1

摘要：

背景：异种脱蛋白骨与宿主骨骼具有相同的结构，抗原性较低，不会或很少引起宿主免疫反应。
目的：观察自制小牛脱蛋白松质骨与兔皮质骨来源成骨细胞的生物相容性。
方法：将自制小牛脱蛋白松质骨与新西兰大白兔成骨细胞复合培养，设单纯成骨细胞对照组，观察细胞生长、支架材料降解及细胞与支架材料附着情况。
结果与结论：小牛脱蛋白松质骨为多孔网状，孔隙互相通连，细胞在其上附着、生长、增殖。复合培养第3天，有较多的细胞黏附并铺展在支架上；第6天，细胞在支架上完全伸展，呈长梭形；第10天细胞分泌较多基质，重叠生长并连接成网状。实验组复合培养9，12 d时成骨细胞的增殖及分泌情况明显优于对照组(P < 0.05)，碱性磷酸酶活性与骨钙素表达高于对照组(P < 0.05)。表明兔皮质骨成骨细胞增殖和成骨能力强，自制的小牛脱蛋白松质骨具有骨诱导性，二者复合具有良好的生物相容性。

关键词: 小牛脱蛋白松质骨, 组织工程, 皮质骨, 成骨细胞, 生物相容性

Abstract:

BACKGROUND: Heterogeneous deproteinized bone has the same structure with the host bone, and the antigen is low causing little or no host immune response.
OBJECTIVE: To observe the biocompatibility of self-made calf eproteinized cancellous bone with rabbit cortical bone-derived osteoblasts.
METHODS: Cells were isolated and cultured with enzyme digestion methods in vitro from the ulnar cortical bone of 3-month-old New Zealand white rabbits. Cells of the 3rd generation were attained for the following experiments. Rabbit cortical bone-derived osteoblasts were co-cultured with self-made calf eproteinized cancellous bone in vitro. Pure osteoblasts were used as controls. The cell growth, scaffold degradation and adherence condition were detected.
RESULTS AND CONCLUSION: The calf eproteinized cancellous bone was porous, and its pores were connected between each other. And with the culture time extended, cells grew and proliferated rapidly. On the 3rd day of co-culture, there were some cells adhered and spread on the scaffold; on the 6th day, cells were fully extended, showing long spindle; on the 10th day, cells secreted lots of extracellular matrix, presented with overlapping growth and were connected into the network. On the 9th and 12th day, the cell proliferation and secretion in the coculture group were superior to the control group (P < 0.05); Alkaline phosphatase activity and osteocalcin level were also higher in the coculture group than the control group (P < 0.05). The self-made calf deproteinized cancellous bone had good biological compatibility with rabbit cortical bone-derived osteoblasts.

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