中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (38): 7036-7040.doi: 10.3969/j.issn.1673-8225.2011.38.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸支架复合骨髓基质细胞修复节段性骨缺损

李  毅1，梁晓军1，王坤正2，陈君长2，杨团民1，同志超1   

  1. 1西安市红十字会医院，陕西省西安市 710054
    2 西安交通大学附属第二医院骨科，陕西省西安市  710004
  • 收稿日期:2011-04-11 修回日期:2011-05-24 出版日期:2011-09-17 发布日期:2011-09-17
  • 作者简介:李毅☆，男，1973年生，陕西省西安市人，汉族，2004年西安交通大学医学院毕业，博士，副主任医师，主要从事组织工程基础研究，足踝外科及关节外科研究临床及基础研究。 liyidoctor@163.com

Repair of long segment bone defects with a novel bone tissue engineering scaffold beta-tricalcium phosphate/calcium polyphosphate fiber/poly-L-lactic acid composite and bone marrow mesenchymal stem cells

Li Yi1, Liang Xiao-jun1, Wang Kun-zheng2, Chen Jun-chang2, Yang Tuan-min1, Tong Zhi-chao1   

  1. 1Red-Cross-Society Hospital of Xi’an City, Xi’an  710054, Shaanxi Province, China
    2Department of Orthopedics, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2011-04-11 Revised:2011-05-24 Online:2011-09-17 Published:2011-09-17
  • About author:Li Yi☆, Doctor, Associate chief physician, Red-Cross-Society Hospital of Xi’an City, Xi’an 710054, Shaanxi Province, China liyidoctor@163.com

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摘要：

背景：组织工程β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸支架材料具有良好的生物相容性。
目的：评估骨髓基质细胞与β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸复合体修复兔桡骨大段骨缺损成骨的效果。
方法：取新西兰大白兔40只，建立桡骨双侧大段骨缺损模型，其中35只右侧植入自体骨髓基质细胞与β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸复合物作为实验组，左侧植入β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸支架材料作为对照组；另5只作为空白对照不作任何处理。植入后4，8，12，16周拍摄X射线片观察骨缺损修复情况。
结果与结论：实验组术后2周可见缺损处有散在的、少量模糊状骨痂生成，术后4周可见明显骨生成影像，成云雾状，均匀分布在骨缺损区，术后8周整个缺损区均可见骨痂生成，成骨现象更加明显，部分髓腔已通，术后12~16周，缺损区已完全被新生骨组织充填，骨髓腔已完全再通，修复区较正常桡骨细，骨缺损修复效果明显优于对照组与空白对照组(P < 0.01)。说明自体骨髓基质细胞与β-磷酸三钙/聚磷酸钙纤维/聚左旋乳酸复合移植可较完全修复大节段骨缺损。

关键词: 骨髓基质细胞, 支架材料, 组织工程, 自体移植, 骨缺损, 修复

Abstract:

BACKGROUND: Bone tissue engineering scaffold beta-tricalcium phosphate/calcium polyphosphate fiber/poly-L-lactic acid (β-TCP/CPPF/PLLA) composite has good biocompatibility.
OBJECTIVE: To explore the effect of β-TCP/CPPF/PLLA scaffold and bone marrow mesenchymal stem cells (BMSCs) in repairing rabbit long segment bone defects.
METHODS: Forty New Zealand white rabbits were divided into BMSCs/β-TCP/CPPF/PLLA group (group A), pure β-TCP/CPPF/PLLA group (group B) and contrast group (group C) randomly. Animal models of long segment radius bone defect were established. The BMSCs/β-TCP/CPPF/PLLA were implanted into the right side of the radius defect (group A), while the pure β-TCP/CPPF/PLLA were implanted into the left side (group B) as control. We did nothing with the contrast group. The rabbits were sacrificed respectively after 4, 8, 12, 16 weeks and the X-ray film was performed at the same time to evaluate the repair effect in different groups.
RESULTS AND CONCLUSION: X-ray film showed there was a small quantity of clouding bony callus in defect region after 2 weeks in group A. Marked osteogenesis screenage could be seen after 4 weeks. The defect region was filled with neonate osseous tissue completely and the bone medullary cavity was completely recanalized during 12-16 weeks. The defect region was filled partly in group B and group C, while group B was significant than group C (P < 0.01). X-ray score revealed that group A was much higher than group B at any time (P < 0.01). The BMSCs/β-TCP/CPPF/PLLA composite were capable of repairing long segment bone defects.

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