中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6905-6908.doi: 10.3969/j.issn.1673-8225.2011.37.016

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

高原低氧大鼠肺组织超微结构及其低氧诱导因子1α的表达

刘  忠1，李文华2   

  1. 1西藏大学医学院，西藏自治区拉萨市  850002
    2西藏民族学院医学院，陕西省咸阳市  712082
  • 收稿日期:2011-03-03 修回日期:2011-06-21 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 李文华，副教授，西藏民族学院医学院，陕西省咸阳市 712082
  • 作者简介:刘忠，1962年生，男，山东省单县人，1984年西藏民族学院医学系毕业，副教授，主要从事高原病理生理学研究。 并列第一作者：李文华，副教授，西藏民族学院医学院，陕西省咸阳市 712082
  • 基金资助:

    课题受西藏科技厅自然科学基金项目(2010)资助。

Lung tissue ultrastructure and hypoxia-inducible factor 1 alpha expression in the rats exposed to high altitude hypoxia

Liu Zhong1, Li Wen-hua2   

  1. 1Medical College of Tibet University, Lhasa  850002, Tibet Autonomous Region, China
    2Medical School of Tibet Institute of Nationalities, Xianyang  712082, Shaanxi Province, China
  • Received:2011-03-03 Revised:2011-06-21 Online:2011-09-10 Published:2011-09-10
  • About author:Liu Zhong, Associate professor, Medical College of Tibet University, Lhasa 850002, Tibet Autonomous Region, China la12sa20@yahoo.com.cn Liu Zhong and Li Wen-hua equally contributed to this paper.
  • Supported by:

    the Natural Science Foundation of Tibet Science and Technology Department, No. 2010*

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摘要：

背景：低氧诱导因子1α可介导哺乳动物细胞适应低氧环境。
目的：观察高原低氧对大鼠肺组织超微结构的影响及其低氧诱导因子1α表达变化。
方法：将SD大鼠分别为进行高原低氧干预1，2，3和30 d，并设置对照组。4个高原低氧组由海拔5 m的西安地区途中耗时1 d带到海拔2 700 m的青海格尔木地区、途中耗时2 d带到海拔5 000 m的唐古拉地区，途中耗时3，30 d分别带到海拔4 500 m的西藏那曲地区。
结果与结论：光镜及电镜观察显示，急性高原低氧2 d组肺组织出现明显的高原肺水肿，急性高原低氧30 d组低氧诱导因子1α mRNA的表达明显增高(P < 0.01)，高原肺水肿现象则明显减轻。结果证实，低氧习服后肺组织低氧诱导因子1α mRNA表达的提高有利于减轻高原肺水肿。

关键词: 高原低氧, 低氧诱导因子1&alpha, 习服, 超微结构, 大鼠, 组织工程

Abstract:

BACKGROUND: Hypoxia-inducible factor 1α may mediate mammalian cells to adapt hypoxia environment.
OBJECTIVE: To observe high altitude hypoxia on the ultrastructure of the lung tissue of hypoxia-inducible factor 1α expression.
METHODS: Sprague-Dawley rats underwent high altitude hypoxia intervention for 1, 2, 3 and 30 days. Control group was set. Four high altitude hypoxia groups consisted of Golmud, Qinghai at the altitude of 2 700 m from 5-m above sea level of Xi'an consuming 1 day, Tanggula region at 5 000 m above sea level consuming 2 days, and Tibet Nagqu at 4 500 m above sea level consuming 3 and 30 days, respectively.
RESULTS AND CONCLUSION: Light and electron microscopy showed that acute high altitude hypoxia 2 day group presented apparent high altitude pulmonary edema, while the 30 day group of acute high altitude hypoxia of high altitude pulmonary edema was significantly reduced, but hypoxia-inducible factor 1α mRNA expression was significantly increased (P < 0.01). Results demonstrate that hypoxia-inducible factor 1α mRNA expression increase in lung tissues after hypoxic acclimatization can help ameliorate high altitude pulmonary edema.

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