中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (34): 6346-6350.doi: 10.3969/j.issn.1673-8225.2011.34.019

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

含氟纳米氟磷灰石糊剂修复人工釉质缺损

王成洁1,2，张翼飞1,2，魏  杰2,3，魏世成1,2   

  1. 1北京大学口腔医学院•口腔医院，交叉学科实验室，口腔颌面外科，北京市 100081
    2北京大学前沿交叉学科研究院生物医用材料与组织工程研究中心，北京市100871
    3上海华东理工大学教育部医用生物材料工程研究中心，上海市  200237
  • 收稿日期:2011-04-14 修回日期:2011-06-07 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 魏杰，博士，副研究员，北京大学前沿交叉学科研究院生物医用材料与组织工程研究中心，北京市 100871；上海华东理工大学教育部医用生物材料工程研究中心，上海市 200237 nic7505@263.net 并列通讯作者：魏世成，博士，教授，北京大学口腔医学院?口腔医院，交叉学科实验室，口腔颌面外科，北京市 100081；北京大学前沿交叉学科研究院生物医用材料与组织工程研究中心，北京市 100871 sc-wei@pku.edu.cn
  • 作者简介:王成洁★，女，1985年生，云南省文山县人，彝族，北京大学口腔医学院在读硕士，主要从事口腔医用纳米材料研究。 dentist_wangcj@163.com
  • 基金资助:

    国家重点基础研究发展计划(973计划)项目，科技部重大科学计划项目纳米计划专项课题 “纳米材料在牙组织再生与修复中的机理研究(2007CB936103)”项目。

Repair of artificial enamel lesions by nano fluorapatite paste containing fluorin

Wang Cheng-jie1, 2, Zhang Yi-fei1, 2, Wei Jie2, 3, Wei Shi-cheng1,2   

  1. 1Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School of Stomatology, Beijing  100081, China
    2Center for Biomedical Materials and Tissue Engineering, Academy for Advanced  Interdisciplinary Stuides, Peking University, Beijing  100871, China
    3Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai  200237, PR China
  • Received:2011-04-14 Revised:2011-06-07 Online:2011-08-20 Published:2011-08-20
  • Contact: Wei Jie, Doctor, Associate researcher, Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Stuides, Peking University, Beijing 100871, China; Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China nic7505@263.net Correspondence to: Wei Shi-cheng, Doctor, Professor, Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School of Stomatology, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Stuides, Peking University, Beijing 100871, China sc-wei@pku.edu.cn
  • About author:Wang Cheng-jie★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, Peking University School of Stomatology, Beijing 100081, China; 2Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Stuides, Peking University, Beijing 100871, China dentist_wangcj@163.com
  • Supported by:

    the National 973 Program of China, No. 2007CB936103*

PDF

289

被引次数

20

摘要：

背景：纳米氟磷灰石糊剂涂敷法可在人牙齿表面生长出类似牙釉质的柱状晶体。
目的：观察1 mg/L人体安全浓度的氟离子对纳米氟磷灰石糊剂修复人工釉质缺损的影响。
方法：将人工早期釉质缺损样本随机分成两组，分别在样本表面涂敷添加含氟的纳米氟磷灰石糊剂(实验组)与不加氟的纳米氟磷灰石糊剂(对照组)，置于37 ℃水浴孵箱中修复。反应前后对牙齿表面行扫描电镜观察，晶体结构分析，化学成分鉴定和韦氏显微硬度测试。
结果与结论：经处理后，两组样品表面都可见有一层与牙釉质化学组成和晶体结构类似的晶体生成，但实验组牙釉质样品表面结晶度、韦氏显微硬度明显高于对照组样品(P < 0.05)，且实验组牙釉质样品表面更光滑。结果表明，人体安全浓度含氟纳米氟磷灰石糊剂可明显提高人工釉质龋显微硬度、结晶度和表面光滑度。

关键词: 纳米氟磷灰石, 氟离子, 修复, 人工釉质缺损, 自组装

Abstract:

BACKGROUND: Enamel-like crystals could grow on human tooth by application of the neutral (pH 7.5) nano-fluorapatite paste.
OBJECTIVE: To evaluate the effect of nano fluorapatite paste with addition of 1 mg/L fluorin on the repair of artificial enamel lesions.
METHODS: Enamel blocks with artificially-induced lesions by 17% phosphate acid solution were randomly divided into two groups that were respectively coated with neutral (pH 7.5) nano-fluorapatite pastes with two different content of fluorin (the group without addition of fluoride ions was set as the controlled group, the other one with addition of 1 mg/L fluoride ions was set as the experimental group) for 15 minutes at 37 ℃. Scanning electronic microscope, XRD, XPS and Vickers micro-hardness test were performed for the enamels before and after treatment.
RESULTS AND CONCLUSION: A layer of enamel-like fluoride substituted hydroxyapatite was observed on the surface of all the samples, but the crytallinity of the experimental group was obviously higher than that of natural enamel and controlled group. But the crystallinity and Vicker micro-hardness of the experimental group was higher than the controlled group obviously (P < 0.05), and the surfaces were smoother. The results indicated the fluorinion had significant effect on the repair of enamel lesion by neutral (pH 7.5) nano-fluorapatite pastes at improvement of Vickers micro-hardness, crystallinity and smoothness of the enamel.

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