中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6207-6210.doi: 10.3969/j.issn.1673-8225.2011.33.031

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

急性肺损伤模型大鼠核因子κB和细胞间黏附分子1表达与川芎嗪的干预

王晶晶，段  斐，张  智，李少春，刘未华，霍  鹏   

  1. 河北大学基础医学院组织胚胎系，河北省保定市 071000
  • 收稿日期:2011-04-22 修回日期:2011-07-09 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 段斐，博士，教授，博士生导师。河北大学基础医学院，河北省保定市 071000 feiduan9999@ sina.com
  • 作者简介:王晶晶★，女，1970年生，汉族，河南省人，河北大学在读硕士，主治医师。主要从事体外冲击波碎石研究。 huayouwjj@ yahoo.com.cn
  • 基金资助:

    课题受河北省人力资源社会保障厅资助项目 (No.20100306)和河北省保定市科技局资助课题[(No.04F001(4)]资助。

Effect of ligustrazine on nuclear factor kappa B and intercellular adhesion molecule-1 expression in a rat model of acute lung injury

Wang Jing-jing, Duan Fei, Zhang Zhi, Li Shao-chun, Liu Wei-hua, Huo Peng   

  1. Department of Histology and Embryology, School of Basic Medical Sciences, Hebei University, Baoding 071000, Hebei Province, China
  • Received:2011-04-22 Revised:2011-07-09 Online:2011-08-13 Published:2011-08-13
  • Contact: Duan Fei, Doctor, Professor, Doctoral supervisor, Department of Histology and Embryology, School of Basic Medical Sciences, Hebei University, Baoding 071000, Hebei Province, China feiduan9999@sina. com
  • About author:Wang Jing-jing★, Studying for master’s degree, Attending physician, Department of Histology and Embryology, School of Basic Medical Sciences, Hebei University, Baoding 071000, Hebei Province, China huayouwjj@yahoo. com.cn
  • Supported by:

    Science Foundation of Human Resource and Social Security Department of Hebei Province, No. 20100306*; a grant from Baoding Science and Technology Bureau, No.04F001(4)*

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摘要：

背景：研究表明，川芎嗪在抗肺纤维化方面发挥重要作用，但对川芎嗪抗急性肺损伤作用及其机制的研究较少。
目的：观察大鼠油酸性急性肺损伤时，核因子κB和细胞间黏附分子1在肺组织中的表达及其变化。
方法：采用静脉注射油酸的方法建立大鼠急性肺损伤模型，建模后并分别用川芎嗪和地塞米松进行干预。
结果与结论：建模成功后，模型组大鼠肺系数明显升高(P < 0.05)。免疫组织化学染色结果显示，模型组肺组织核因子κB和细胞间黏附分子1的表达明显增强(P < 0.05)；应用川芎嗪和地塞米松干预后上述变化明显减轻(P < 0.05)。证实川芎嗪对急性肺损伤时的肺组织起到明显的保护作用，其保护机制与川芎嗪抑制核因子κB和细胞间黏附分子1的表达有关。

关键词: 川芎嗪, 急性肺损伤, 核因子κB, 细胞间黏附分子1, 油酸, 动物模型, 组织工程

Abstract:

BACKGROUND: Ligustrazine is quite effective in treating and preventing idiopathic pulmonary fibrosis (IPF), but there are few reports describing the mechanism by which ligustrazine treats acute lung injury.
OBJECTIVE: To observe the expression changes of nuclear factor-kappa B (NF-κB) and intercellular adhesion molecule-1(ICAM-1) in lung tissues of rats with acute lung injury induced by oleic acid.
METHODS: Rat models of acute lung injury were established by intravenous injection of oleic acid and then interfered with ligustrazine and dexamethasone.
RESULTS AND CONCLUSION: After successful induction of acute lung injury, the lung coefficient increased significantly in the model group compared with the control group (P < 0.05). Immunohistochemical staining results showed that NF-κB and ICAM-1 expression in the model group was significantly increased (P < 0.05). After ligustrazine and dexamethasone intervention, the above-mentioned changes were obviously alleviated (P < 0.05). These results suggest that ligustrazine exhibits an obvious protective effect on lung tissue during acute lung injury, and the mechanism might be related to the inhibition of NF-κB and ICAM-1 expression.

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