中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6191-6195.doi: 10.3969/j.issn.1673-8225.2011.33.027

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

紫外激光辐射区肝组织β连环蛋白及过氧化物酶体增殖物激活受体γ蛋白的表达

王  杨1, 2，王旭葆3，董战玲2，戚  燕4，王  晗2，符史干2   

  1. 海南医学院，1康复基础医学教研室，2生理学教研室，海南省海口市 571101；3北京工业大学激光工程研究院，北京市 100124；4中国电子科技集团公司第十一研究所，北京市  100015
  • 收稿日期:2011-03-05 修回日期:2011-06-07 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 符史干，男，医学博士，教授。专业方向：循环生理学。海南医学院生理学教研室，海南省海口市 571101 nordicflash@ tom.com
  • 作者简介:王杨☆，男，1963年生，2000年日本国立筑波大学医学院毕业，博士，北京市人，汉族。副教授，主要从事运动医学与康复，特殊环境生理学研究。 katotds@sina. com 并列第一作者：王旭葆☆，男，1972年生，2006年北京工业大学激光工程研究院毕业，博士，黑龙江省庆安县人，汉族，副研究员，主要从事激光先进加工技术研究。 wangxubao@ bjut.edu.cn
  • 基金资助:

    课题受海南省教育厅高校科研资助项目(Hjkj2008-42)资助。

Beta-catenin and peroxidase proliferator-activated receptor gamma expressions in hepatic tissue treated by 355 nm laser ablation

Wang Yang1,2, Wang Xu-bao3, Dong Zhan-ling2, Qi Yan 4, Wang Han2, Fu Shi-gan2   

  1. 1Division of Rehabilitation Medicine, 2Department of Physiology, Hainan Medical College, Haikou  571101, Hainan Province, China; 3Institute of Laser Engineering, Beijing University of Technology, Beijing 100124, China; 4Department of Civilian Products, North China Research Institute of Electro-optics, Beijing 100015, China
  • Received:2011-03-05 Revised:2011-06-07 Online:2011-08-13 Published:2011-08-13
  • Contact: Fu Shi-gan, M.D., Professor, Department of Physiology, Hainan Medical College, Haikou 571010, Hainan Province, China
  • About author:Wang Yang☆, Doctor, Associate professor, Division of Rehabilitation Medicine and Department of Physiology, Hainan Medical College, Haikou 571101, Hainan Province, China katotds@sina.com Wang Xu-bao, Doctor, Associate researcher, Institute of Laser Engineering, Beijing University of Technology, Beijing 100124, China wangxubao@ bjut.edu.cn Wang Yang and Wang Xu-bao contributed equally to this paper and were considered as co-first authors.
  • Supported by:

    a grant from Education Department Science Research Foundation of Hainan Province for Higher Education Institutes, No. Hjkj2008-42*

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摘要：

背景：研究表明经典的wnt信号途径可通过抑制成脂分化关键因子过氧化物酶体增殖物激活受体γ而抑制细胞向脂肪细胞的分化。
目的：观察紫外光辐射肝组织致创后自然恢复期不同阶段β连环蛋白及过氧化物酶体增殖物激活受体γ的表达。
方法：将雄性SD大鼠随机分4组：激光致创组大鼠是以355 nm紫外激光辐射肝叶形成损伤区，器械致创对照组则以手术剪肝叶致创，假手术组仅做腹部切开及缝合，正常组大鼠仅实施正常喂养。
结果与结论：紫外激光可在肝组织形成边缘整齐的消融区并在周围形成可自然修复的有限致损区域。免疫组织化学图像平面测量表明紫外激光辐射后自然恢复1周大鼠肝组织中β连环蛋白表达低于各对照组(P < 0.01)，而自然恢复表达β连环蛋白 辐射区周围组织表达高于正常(P < 0.01)。蛋白印迹结果显示辐射致创组辐射后自然同时过氧化物酶体增殖物激活受体γ蛋白2周 恢复高于1周恢复(P < 0.01)，且恢复期肝组织中可同时表达过氧化物酶体增殖物激活受体γ 1及2。结果证实，辐射区旁组织自然恢复各阶段存在不同的β连环蛋白及过氧化物酶体增殖物激活受体γ表达调控，2周自然恢复后辐射区旁组织成脂进程得到加强。

关键词: 紫外激光消融, β连环蛋白, 过氧化物酶体增殖物激活受体γ, 大鼠, 组织工程

Abstract:

BACKGROUND: Several studies have demonstrated that wnt signaling pathway inhibits adipogenic differentiation through inhibiting the expression of peroxisome proliferator-activated receptor gamma (PPARγ), a key factor of adipogenic differentiation in hepatic tissue.
OBJECTIVE: To determine the expressions of β-catenin and PPARγ in hepatic tissue treated by 355 nm laser ablation at different stages of natural recovery stage.
METHODS: Male SD rats were randomized divided to four groups. In the ultraviolet beam ablation group, 355 nm ultraviolet beam was used on hepatic tissue targeted zone to obtain an ablation damage area. In the mechanical damage group, mechanical damage was created through the use of operating scissors. In the sham operation group, abdominal incision and suture were performed, without other procedures. In the normal control group, rats were routinely raised without any procedures.
RESULTS AND CONCLUSION: Hepatic tissue obtained a regular ablation damage zone and a recoverable para-damage area by ultraviolet laser beam radiation. Immunohistochemistry results showed that β-catenin expression was significantly lower after 1-week natural recovery in the ultraviolet beam ablation group compared with other groups (P < 0.01), but its expression was significantly higher in the para-damage area after 2-week recovery compared with the normal control group (P < 0.01). Western blot assay showed that PPARγ expression in the ultraviolet beam ablation group was significantly increased after 2-week natural recovery than 1-week natural recovery (P < 0.01), and PPARγ had a co-expression with its iosform 1 and 2 during the natural recovery stage. The results suggest that β-catenin and PPARγ expression pattern tightly depended on nature recovery duration, and adipogenic process in the para-damage area in the hepatic tissue was strengthened after 2-week natural recovery.

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