中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5213-5216.doi: 10.3969/j.issn.1673-8225.2011.28.021

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

博来霉素气管灌注构建肺纤维化模型大鼠肺组织低氧诱导因子1α的动态表达

何平平1，张  平1，王在岩2，李婉霜2，肖新洲2   

  1. 1 南华大学护理学院，湖南省衡阳市  421001
    2 南华大学附属第一医院呼吸内科，湖南省衡阳市  421001
  • 收稿日期:2011-04-10 修回日期:2011-05-20 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 张平，主任医师，教授，硕士生导师，南华大学护理学院，湖南省衡阳市 421001 ZP9707@sohu. com
  • 作者简介:何平平★，女，1979年生，湖南省湘潭市人，汉族，2002年南华大学毕业，硕士，讲师，主要从事呼吸系统疾病的研究。 hpp-612@163. com
  • 基金资助:

    湖南省自然科学基金资助项目(08JJ5003)，课题名称：肺纤维化基因时空表达谱及黄芪甲甙干预的研究；湖南省财政厅/教育厅基金资助重点项目(10A107)，课题名称：ADAMTS-1在肺纤维化中的作用及其机制研究；湖南省财政厅/教育厅基金资助一般项目(10C1160)，课题名称：白黎芦醇逆转肺纤维化作用及MAPK/ERK信号转导特征研究；衡阳市科技发展基金资助项目(2010ks33)，课题名称：白藜芦醇对大鼠肺纤维化组织中HIF-1α和NF-κB的表达影响。

Dynamic expression of hypoxia inducible factor-1 alpha in lung tissues of a rat model of pulmonary fibrosis induced by intratracheal instillation with bleomycin

He Ping-ping1, Zhang Ping1, Wang Zai-yan2, Li Wan-shuang2, Xiao Xin-zhou2   

  1. 1School of Nursing, University of South China, Hengyang   421001, Hunan Province, China
    2Department of Respiratory Disease, First Affiliated Hospital, University of South China, Hengyang   421001, Hunan Province, China
  • Received:2011-04-10 Revised:2011-05-20 Online:2011-07-09 Published:2011-07-09
  • Contact: Zhang Ping, Chief physician, Professor, Master’s supervisor, School of Nursing, University of South China, Hengyang 421001, Hunan Province, China ZP9707@sohu.com
  • About author:He Ping-ping★, Master, Lecturer, School of Nursing, University of South China, Hengyang 421001, Hunan Province, China hpp-612@163.com
  • Supported by:

    the Natural Science Foundation of Hunan Province, No.08JJ5003*; a grant from Hunan Provincial Department of Finance/Education of Hunan Province (Key Program), No. 10A107*; a grant from Department of Finance/Education of Hunan Province (General Program), No. 10C1160*; Science and Technology Development Fund of Hengyang City, No. 2010ks33* 

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摘要：

背景：低氧诱导因子1α是介导低氧反应的核转录因子，其对肺纤维化的作用尚不明确。
目的：观察低氧诱导因子1α在肺纤维化发病中的作用。
方法：采用博来霉素(5 mg/kg)一次性气管内灌注建立SD大鼠肺纤维化模型。造模后7，14，28 d观察大鼠肺组织的病理学改变及肺组织中低氧诱导因子1α蛋白及mRNA的动态表达。
结果与结论：博来霉素灌注后，大鼠肺组织炎症反应明显，并逐渐出现纤维化及胶原纤维沉积。免疫组织化学及RT-PCR结果显示，在大鼠肺纤维化发病过程中，低氧诱导因子1α蛋白及mRNA的表达趋势相同，即随造模时间的延长表达逐渐增多，并于造模后第14天达高峰。提示低氧诱导因子1α在大鼠肺纤维化发病过程中起重要作用，其过度表达可能参与了肺纤维化的发生与发展。

关键词: 博来霉素, 低氧诱导因子1α, 肺纤维化, 动物模型, 大鼠

Abstract:

BACKGROUND: Hypoxia inducible factor 1α(HIF-1α) is a nuclear transcription factor to mediate hypoxia reaction. However, the effects of HIF-1α on lung fibrosis remain poorly understood.
OBJECTIVE: To observe the effects of HIF-1α in the pathogenesis of pulmonary fibrosis．
METHODS: Rat pulmonary fibrosis was induced by an intratracheal injection of bleomycin (5 mg/kg). The pathogenetic procedure of pulmonary fibrosis and dynamic expression of HIF-1α in lung tissues were observed through some methods including hematoxylin-eosin and Masson's trichrome of lung section, immunohistochemical staining of HIF-1α protein in the lung, RT-PCR of HIF-1α mRNA in the lung on the 7th,14th, and 28th days．
RESULTS AND CONCLUSION: In the pathogenetic procedure of pulmonary fibrosis in rats, compared with the normal group, the protein and mRNA expression of HIF-1α in the model group increased on the 7th day (10.33±0.31, 21.10±1.6, P < 0.05) and the 14th day (17.65±1.82, 64.93±4.88, P < 0.05), but decreased on the 28th day (13.48±1.45, 47.85±3.40, P < 0.05). HIF-1α protein and mRNA was dynamically expressed in the lung tissue of rats with experimental pulmonary fibrosis, which may contribute to the pathogenesis of pulmonary fibrosis.

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