中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5201-5204.doi: 10.3969/j.issn.1673-8225.2011.28.018

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

肥大细胞在腺嘌呤致慢性肾功能衰竭模型大鼠肾组织中的浸润

邢红梅1，于彩云2，刘雪梅1，刘洪彦1   

  1. 1青岛大学医学院附属医院肾内科，山东省青岛市  266003
    2青岛农业大学校医院，山东省青岛市 266109
  • 收稿日期:2011-01-23 修回日期:2011-02-28 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 刘雪梅，博士，副主任医师，青岛大学医学院附属医院肾内科，山东省青岛市 266003 Liuxm2004@ sina.com
  • 作者简介:邢红梅★，女，1980年生，山东省蓬莱市人，汉族，青岛大学医学院在读硕士，蓬莱市人民医院主治医师，主要从事慢性肾病的防治研究。 plxhm@sina.com
  • 基金资助:

    山东省科技攻关项目资助课题(项目编号：2010G0020222)，课题名称：干细胞因子与慢性肾脏疾病的关系研究。

Infiltration of mast cells in renal interstitium in a rat model of adenine-induced chronic renal failure

Xing Hong-mei1, Yu Cai-yun2, Liu Xue-mei1, Liu Hong-yan1   

  1. 1Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao   266003, Shandong Province, China
    2 Hospital of Qingdao Agricultural University, Qingdao 266109  Shandong Province, China
  • Received:2011-01-23 Revised:2011-02-28 Online:2011-07-09 Published:2011-07-09
  • Contact: Liu Xue-mei, Doctor, Associate chief physician, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China Liuxm2004@sina. com
  • About author:Xing Hong-mei★, Studying for master’s degree, Attending physician, Department of Nephrology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China plxhm@sina.com
  • Supported by:

    Scientific and Technological Project of Shandong Province, No. 2010G0020222*

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摘要：

背景：近年研究提示肥大细胞的浸润与人类多种肾病患者的肾间质纤维化关系密切。肥大细胞是否参与了腺嘌呤致慢性肾功能衰竭大鼠模型肾间质纤维化？作者未检索到此类报道。
目的：探讨肥大细胞在腺嘌呤致慢性肾功能衰竭大鼠模型肾组织中的分布特点及其与肾间质纤维化之间的关系。
方法：46只雄性Wistar大鼠，随机分成对照组和模型组。模型组予腺嘌呤灌胃，剂量为150 mg/(kg•d)；对照组以等量生理盐水灌胃。分别于不同时间点检测血尿指标，并对肾组织进行苏木精-伊红染色、Masson染色及肾小管间质纤维化评分；采用甲苯胺蓝和免疫组化方法观察肥大细胞在肾脏的分布及浸润数量，并分析它们与肾间质纤维化的相关性。
结果与结论：模型组大鼠随着灌胃时间的延长，尿蛋白/ 尿肌酐、血清肌酐和血清尿素氮持续升高，肾间质纤维化评分也逐渐增加，不同时间点之间及其与对照组比较，差异均有显著性意义(P < 0.01)；肥大细胞主要分布在模型鼠的肾小管间质、肾小球囊外及血管周围，间质纤维化较重区域浸润较多，其浸润数量随着模型鼠肾损害的加重逐渐增加，不同时间点之间比较，差异均有显著性意义(P < 0.01)，并且与肾间质纤维程度呈显著正相关(r =0.96，P < 0.001)。提示肥大细胞可能促进了腺嘌呤致慢性肾功能衰竭大鼠模型肾间质纤维化的进展。

关键词: 肥大细胞, 慢性肾功能衰竭, 肾间质纤维化, 类胰蛋白酶, 大鼠, 动物模型

Abstract:

BACKGROUND: Recent studies have shown that infiltration of mast cells (MCs) is closely correlated with the degree of tubulointerstitial fibrosis in various renal diseases. Whether MCs are involved in renal interstitial fibrosis in a rat model of adenine-induced chronic renal failure is poorly understood.
OBJECTIVE: To investigate the distributing characteristics of MCs and the relationship between number of MCs in the interstitium and tubulointerstitial fibrosis.
METHODS: Forty-six male Wistar rats were randomly divided into control group and model group. The model group rats were intragastrically administered adenine at a dose of 150 mg/kg per day, and the control group rats were intragastrically administered the same amount of physiological saline. Blood and urine were collected for measuring creatinine and protein. Kidney sample sections were stained with hematoxylin and eosin, Masson’s trichrome. Renal interstitial fibrosis (RIF) was assessed by light microscope. The intensity and distribution of MCs were examined by toluidine blue and immunohistochemistry using tryptase antibody.
RESULTS AND CONCLUSION: Proteinuria and renal function of model rats deteriorated gradually with the elongation of intragastric administration time, and the score of RIF increased gradually. MCs were detected in the interstitium, periglomerular and perivascular areas in the model kidneys, in close proximity to areas of interstitial fibrosis, but were not found in the glomeruli. With aggravation of interstitial lesions degree, the number of MCs increased. There were significant differences in number of MCs, RIF score, urinary protein, serum creatinine and blood urea nitrogen between the model rats and control rats at different time points (P < 0.01). MCs were positively correlated with interstitial fibrosis (r = 0.96, P < 0.001). These findings suggest that increased infiltration of MCs is involved in renal interstitial fibrosis in a rat model of adenine-induced chronic renal failure, which provides an ideal animal model for the further research of MCs in renal tissue.

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