中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (28): 5193-5196.doi: 10.3969/j.issn.1673-8225.2011.28.016

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

一种效果确切的大鼠重症急性胰腺炎模型

郝建志，方驰华，范应方，黄燕鹏，胡海北   

  1. 南方医科大学珠江医院肝胆一科，广东省广州市 510282
  • 收稿日期:2011-01-15 修回日期:2011-02-10 出版日期:2011-07-09 发布日期:2011-07-09
  • 通讯作者: 方驰华，博士，主任医师，教授，博士生导师，南方医科大学珠江医院肝胆一科，广东省广州市 510282 fangch_dr@126.com
  • 作者简介:郝建志★，在读硕士，医师，主要从事肝胆胰外科基础研究及临床研究。

An effective rat model of severe acute pancreatitis

Hao Jian-zhi, Fang Chi-hua, Fan Ying-fang, Huang Yan-peng, Hu Hai-bei   

  1. First Department of Hepatobilliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-01-15 Revised:2011-02-10 Online:2011-07-09 Published:2011-07-09
  • Contact: Fang Chi-hua, Doctor, Chief physician, Professor, Doctoral supervisor, First Department of Hepatobilliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China fangch_dr@126.com
  • About author:Hao Jian-zhi★, Studying for master’s degree, Physician, First Department of Hepatobilliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China

PDF

425

被引次数

15

摘要：

背景：目前尚无任何一种重症急性胰腺炎动物模型能与人类重症急性胰腺炎的发病过程完全一致，而用于实验治疗性研究的模型更是少之又少。
目的：建立一种大鼠重症急性胰腺炎模型，为进行大鼠重症急性胰腺炎的治疗性研究提供前提条件。
方法：30只SD大鼠随机分为对照组和模型组，对照组不建模，模型组大鼠采用逆行胰管穿刺注射法建立重症急性胰腺炎模型，建模后6，12，24 h，测血、腹水淀粉酶、血白细胞数量、血清白细胞介素6、肿瘤坏死因子α表达，并对胰腺组织进行病理评分。
结果与结论：模型组大鼠存活率80%，对照组大鼠存活率100%；模型组大鼠随时间的延长，病理评分、血、腹水淀粉酶、白细胞、白细胞介素6、肿瘤坏死因子α逐渐升高(P < 0.05)。说明逆行胰管注射法能够成功建立大鼠重症急性胰腺炎模型。

关键词: 重症急性胰腺炎, 逆行胰管注射法, 大鼠, 动物模型, 白细胞介素6, 肿瘤坏死因子α

Abstract:

BACKGROUND: There is no animal model of severe acute pancreatitis that has the pathogenesis as same as human. Moreover, there are even fewer models used in experimental treatment studies.
OBJECTIVE: To construct an effective rat model of severe acute pancreatitis for the therapeutic study on severe acute pancreatitis rats.
METHODS: Thirty rats were randomly divided into control and model groups. The severe acute pancreatitis model was constructed by retrograde pancreatic duct infusion of Na-taurocholate. The expression of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α)  in the serum as well as amylase in the ascites and blood and white cell number in the blood were detected, and the pathological changes of pancreatic tissues were observed.
RESULTS AND CONCLUSION: The survival rate was 80% in the model group, and 100% in the control group. With increasing time, pathological score, IL-6 and TNF-α expression in the serum, white cell number and amylase in the ascites and blood were increased gradually (P < 0.05). It was indicated that the severe acute pancreatitis model was constructed successfully in rats.

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