中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (12): 2171-2174.doi: 10.3969/j.issn.1673-8225.2011.12.020

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的细胞相容性

梁  爽1，李  权1，唐休发2，冯  扬1，何等旗3   

  1. 1口腔疾病研究国家重点实验室，四川省成都市 610041
    2华西口腔医院头颈肿瘤外科，四川省成都市 610041
    3兰州大学第二附属医院口腔科，甘肃省兰州市  730030
  • 收稿日期:2010-10-15 修回日期:2010-11-21 出版日期:2011-03-19 发布日期:2011-03-19
  • 通讯作者: 唐休发，教授，博士生导师，华西口腔医院头颈肿瘤外科，四川省成都市 610041
  • 作者简介:梁爽★，女，1986年生，重庆市人，汉族，四川大学华西口腔医学院在读硕士，主要从事骨骼肌组织工程支架材料研究。 nancy1986xx@126.com
  • 基金资助:

    教育部博士学科点基金项目(20050610077)和国家自然科学基金资助项目(30872890)。

Cytocompatibility of electro-spinning nano-fibrous scaffolds for skeletal muscle tissue engineering

Liang Shuang1, Li Quan1, Tang Xiu-fa2, Feng Yang1, He Deng-qi3   

  1. 1State Key Laboratory of Oral Disease, Chengdu  610041, Sichuan Province, China
    2Department of Head and Neck Oncology, West China College of Stomatology, Sichuan University, Chengdu   610041, Sichuan Province, China
    3Department of Stomatology, the Second Affiliated Hospital of Lanzhou University, Lanzhou   730030, Gansu Province, China
  • Received:2010-10-15 Revised:2010-11-21 Online:2011-03-19 Published:2011-03-19
  • Contact: Tang Xiu-fa, Professor, Doctoral supervisor, Department of Head and Neck Oncology, West China College of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Liang Shuang★, Studying for master’s degree, State Key Laboratory of Oral Disease, Chengdu 610041, Sichuan Province, China nancy1986xx@126.com
  • Supported by:

    Doctor Subject Foundation of the Ministry of Education of China, No. 20050610077*; the National Natural Science Foundation of China, No. 30872890*

PDF

440

被引次数

5

摘要：

背景：有报道以生物可降解的胶原盘或聚L-乳酸、聚羟基乙酸、聚L-乳酸/聚羟基乙酸共聚物等作为骨骼肌组织工程的支架材料，各有优缺点，不能完全满足骨骼肌组织工程的需要。
目的：探讨静电纺丝纳米纤维膜作为骨骼肌组织工程支架材料的可行性。
方法：制备7种不同组分的静电纺丝纳米纤维膜，以其浸提液为培养基培养第3代SD乳鼠成肌细胞，以含体积分数20%新生小牛血清的F12培养基培养的为对照。采用MTT法和扫描电镜检测成肌细胞在各组材料的黏附及生长情况。
结果与结论：各组分静电纺丝纳米纤维膜吸光度值与对照组间差异无显著性意义(P > 0.05)。各组分静电纺丝纳米纤维膜组成肌细胞黏附率差异有显著性意义(P < 0.05)。扫描电镜与上述结果一致。含70%聚乳酸+20%蚕丝蛋白+10%胶原组成电纺丝纳米纤维膜组可见大量成肌细胞黏附，呈梭形，两极伸展，排列规律，效果最好。其他各组细胞少，形态不规则，似衰退期成肌细胞。提示静电纺丝纳米纤维膜无细胞毒性，对成肌细胞的增殖无影响，成肌细胞能良好地黏附；以70%聚乳酸+ 20%蚕丝蛋白+10%胶原组分效果最佳。

关键词: 聚乳酸, 蚕丝蛋白, 胶原, 成肌细胞, 静电纺丝技术, 纳米纤维膜, 组织工程

Abstract:

BACKGROUND: Some scholars reported that biodegradable collagen plate or poly L-lactic acid (PLLA), poly glycolic acid (PGA), PLLA/PGA copolymer, serve as scaffold materials of skeletal muscle tissue engineering. But these materials have their own advantages and disadvantages, and still can not fulfill the needs of skeletal muscle tissue engineering.
OBJECTIVE: To study the feasibility of the electro-spinning nano-fibrous membrane as scaffolds for skeletal muscle tissue engineering.
METHODS: The rat myoblasts at the third passage were cultured with extracts obtaining from different kinds of electro-spinning nano-fibrous membranes (7 groups), with an extractant of F12 media containing 20% bovine calf serum. The adhesion and growth of myoblasts on the nano-fibrous membrane were examined with MTT and scanning electron microscopy methods.
RESULTS AND CONCLUSION: The absorbance value between any two groups has no statistically significant difference compared with control group (P > 0.05), while obvious statistical differences of adhesion rate lay in different groups of biomaterials (P < 0.05). The same result lies in the scanning electron microscopy image. A great quantity of myoblasts adhered to the 70% PLA + 20% silk fibroin + 10% collagen group. These myoblasts are spindle-shaped, regularly arranged, and connecting to adjacent cells. In other groups, the myoblasts are less and irregular, much more like myoblasts in recession. The electro-spinning nano-fibrous membrane has no toxicity and no bad effect on the growth of myoblasts. They adhere well on the membrane, especially on the 70% PLA + 20% silk fibroin + 10% collagen group.

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