中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1459-1462.doi: 10.3969/j.issn.1673-8225.2011.08.031

• 生物材料学术探讨 biomaterial academic discussion • 上一篇    下一篇

阳离子脂质体介导的基因转移机制

王  冰1, 2，张树彪2，周集体1，赵不凋2，杨宝灵2，崔绍辉2，赵轶男2   

  1. 1大连理工大学环境与生命学院，辽宁省大连市116024
    2大连民族学院国家民委-教育部重点实验室，辽宁省大连市116600
  • 收稿日期:2010-09-16 修回日期:2010-12-17 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 张树彪，博士，教授，大连民族学院国家民 委-教育部重点实验室，辽宁省大连市 116600 zsb@dlnu.edu.cn 周集体，博士，教授，连理工大学环境与生命学院，辽宁省大连市 116024 zji@dlut.edu.cn
  • 作者简介:王冰☆，女，1975 年生，辽宁省朝阳市人，汉族，大连理工大学环境与生命学院在读博士，讲师，主要从事环境生物工程和非病毒基因载体的研究。 wangbing@dlnu.edu.cn
  • 基金资助:

    课题受国家自然科学基金资助项目(20876027) 和大连民族学院青年基金资助项目(2008A202)资助。

Mechanisms of cationic liposome-mediated gene transfer

Wang Bing1, 2, Zhang Shu-biao2, Zhou Ji-ti1, Zhao Bu-diao2, Yang Bao-ling2, Cui Shao-hui2, Zhao Yi-nan2   

  1. 1School of Environmental and Biological Science, Dalian University of Technology, Dalian   116024, Liaoning Province, China
    2Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian   116600, Liaoning Province, China
  • Received:2010-09-16 Revised:2010-12-17 Online:2011-02-19 Published:2011-02-19
  • Contact: Zhang Shu-biao, Doctor, Professor, Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian 116600, Liaoning Province, China zsb@dlnu.edu.cn Zhou Ji-ti, Doctor, Professor, School of Environmental and Biological Science, Dalian University of Technology, Dalian 116024, Liaoning Province, China zji@dlut.edu.cn
  • About author:Wang Bing☆, Studying for doctorate, Lecturer, School of Environmental and Biological Science, Dalian University of Technology, Dalian 116024, Liaoning Province, China; Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, Dalian Nationalities University, Dalian 116600, Liaoning Province, China wangbing@dlnu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 20876027*; Youth Foundation of Dalian Nationalities University, No. 2008A202*

PDF

421

被引次数

26

摘要：

背景：与病毒载体相比，许多天然与合成的阳离子类脂以脂质体的形式用于基因转移，具有无免疫原性、易生产、质粒免受核酸酶降解和无致瘤性等优点，并且作为病毒载体的有效替代物，阳离子脂质体能用于细胞的体内和体外转染。
目的：介绍阳离子脂质体介导的基因转移机制研究进展。
方法：由第一作者用计算机检索中国期刊全文数据库(CNKI：1987/2010)和PubMed (1987/2010)数据库，检索词分别为“基因治疗、阳离子脂质体、基因转移、机制”和“gene therapy, cationic liposome, gene transfer, mechanism”，语言分别设定为中文和英文。从阳离子脂质体基因转染和基因转移机制进行总结，综述了阳离子脂质体介导的基因转移机制。
结果与结论：共检索到108篇，按纳入和排除标准对文献进行筛选，共纳入20篇文章。综述了阳离子脂质体介导的基因转移机制，包括阳离子脂质体/DNA复合物的形成、细胞吸收、内含体释放和复合物解体以及细胞核摄入等方面的研究内容。结果提示，对类脂构效关系和基因转移机制的研究，是提高阳离子脂质体转染效率和优化基因治疗的关键。

关键词: 基因治疗, 阳离子脂质体, 基因转移, 基因转染, 组织工程

Abstract:

BACKGROUND: Compared with viral vector, many cationic lipids naturally occurred or synthesized have been used for gene transfer in the form of liposomes, which have the advantages of non-immunogenicity, simple production, plasmid protected against nuclease degradation and non-oncogenicity, etc. And cationic lipids as an effective alternative of viral vector, cationic liposomes can be used for cell transfection in vivo and in vitro.
OBJECTIVE: To introduce research progress on mechanisms of cationic liposome-mediated gene transfer.
METHODS: CNKI database from 1987 to 2010 and PubMed database from 1987 to 2010 were retrieved by the first author with computer. The index words were “gene therapy, cationic liposome, gene transfer, mechanism” in Chinese and English. Literatures were limited to Chinese and English languages. From cationic liposome gene transfer and gene transfer mechanisms were summarized, the cationic liposome-mediated gene transfer mechanisms were reviewed.
RESULTS AND CONCLUSION: Totally 108 literature were retrieved and selected according to inclusion and exclusion criteria, 20 of them were included. The cationic liposome-mediated gene transfer mechanisms were reviewed, including the formation of the cationic lipid / DNA complexes, cell uptake, inclusion body release, complex dissolution, and nucleus intake research. The results indicated that research on structure-activity relationship of lipids and the mechanism of gene transfer is the key to improve cationic liposome transfection efficiency and optimize gene therapy.

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