中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (6): 1030-1034.doi: 10.3969/j.issn.1673-8225.2011.06.018

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞对慢性哮喘小鼠血清白细胞介素12和白细胞介素4水平的影响

戈霞晖1，白  冲1，陈若华2，胡珍丽1，朱天怡1，李  强1   

  1. 上海市长海医院，1呼吸内科，2特需诊疗科，上海市  200433
  • 收稿日期:2010-08-27 修回日期:2010-10-12 出版日期:2011-02-05 发布日期:2011-02-05
  • 通讯作者: 白冲，博士，主任医师，上海市长海医院呼吸内科，上海市 200433
  • 作者简介:戈霞晖☆，女，1975年生，江苏省如皋市人，汉族，解放军第二军医大学呼吸内科在读博士，主要从事间充质干细胞与哮喘的研究。 julian1128@126.com
  • 基金资助:

    国家自然科学基金(30871133)。课题名称：骨髓间充质干细胞移植对慢性阻塞性肺病气道及肺损伤的修复作用及相关机制。

Effects of bone marrow mesenchymal stem cells on the serum levels of interleukin-4 and interleukin-12 in chronic asthmatic mice

Ge Xia-hui1, Bai Chong1, Chen Ruo-hua2, Hu Zhen-li1, Zhu Tian-yi1, Li Qiang1   

  1. 1Department of Respiratory Medicine, 2Department of Special Clinic, Changhai Hospital, Shanghai  200433, China
  • Received:2010-08-27 Revised:2010-10-12 Online:2011-02-05 Published:2011-02-05
  • Contact: Bai Chong, Doctor, Chief physician, Department of Respiratory Medicine, Changhai Hospital, Shanghai 200433, China
  • About author:Ge Xia-hui☆, Studying for doctorate, Department of Respiratory Medicine, Changhai Hospital, Shanghai 200433, China julian1128@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30871133*

PDF

285

被引次数

9

摘要：

背景：研究显示，骨髓间充质干细胞移植通过减轻炎症程度来改善急性肺损伤的病情，而骨髓间充质干细胞移植对哮喘疾病的研究甚少。
目的：观察慢性哮喘小鼠移植同种异体骨髓间充质干细胞后，其血清白细胞介素12和白细胞介素4水平的变化。
方法：取40只雌性BALB/c小鼠，随机分为4组，正常对照组和骨髓间充质干细胞对照组以PBS致敏及激发小鼠，于第21天激发前气管内注射PBS或骨髓间充质干细胞 30 μL。哮喘模型组和骨髓间充质干细胞治疗组用鸡卵白蛋白制备慢性哮喘模型，于第21天激发前气管内注射PBS或骨髓间充质干细胞 30 μL。采用ELISA法检测各组小鼠血清炎症因子水平。
结果与结论：病理提示哮喘模型组支气管上皮黏膜脱落，同时上皮黏膜有杯状细胞增生，部分管腔内大量黏液栓塞；气道、血管旁有大量炎性细胞浸润以及气道平滑肌细胞增生和肥大。正常对照组及骨髓间充质干细胞对照组无气道炎症及气道重塑的表现，而骨髓间充质干细胞治疗组气道炎症及气道重塑明显减轻。对比骨髓间充质干细胞对照组及正常对照组，哮喘模型组白细胞介素12明显减低，白细胞介素13 和白细胞介素4明显增高；而骨髓间充质干细胞治疗组较哮喘模型组白细胞介素12明显升高，白细胞介素4明显降低。结果表明骨髓间充质干细胞治疗哮喘可能通过降低白细胞介素4水平，提高体内白细胞介素12水平，从而改善气道炎症及气道重塑的程度。

关键词: 哮喘, 骨髓间充质干细胞, 白细胞介素12, 白细胞介素4, 气道局部移植

Abstract:

BACKGROUND: Previous studies have demonstrated that anti-in?ammatory actions of bone marrow mesenchymal stem cells (BMSCs) could ameliorate severity of acute lung injury. However, only a few studies have addressed BMSCs transplantation for asthma.
OBJECTIVE: To study the effect of allogeneic BMSCs transplantation on the serum levels of interleukin-12 (IL-12) and IL-4 in chronic asthmatic mice.
METHODS: Forty female BALB/c mice were equally randomized into four groups. Mice of normal control and BMSCs control groups received PBS sensitization to arouse mice. On day 21 before suscitation, PBS or BMSCs (30 μL) were injected via the intratracheal injection. Mice of asthma model and BMSCs treatment groups were used to establish chronic asthma using chicken ovalbumin. On day 21 before suscitation, PBS or BMSCs (30 μL) were injected via the intratracheal injection. Serum inflammatory factor levels were measured in mice of each group using enzyme linked immunosorbent assay.
RESULTS AND CONCLUSION: There were a great number of inflammatory cell infiltrations around airway and vessel, in conjunction with epithelial proliferation and partial breakage and defluvium, patchy airway occlusion by hyperviscous mucus and hypertrophy and hyperplasia of airway smooth muscle in the asthma model group. However, there was no obvious inflammatory cells infiltration in the airway and airway remodeling both in normal control group and BMSCs control group. Airway inflammation and airway remodeling were significantly relieved in the BMSCs treatment group. Compared to the normal control group and BMSCs control group, low level of IL-12 and high levels of IL-13 and IL-4 were present in the asthma model group. Transplantation of BMSCs significantly elevated level of IL-12 and decreased level of IL-4 in serum. Results suggest that BMSCs for asthma can relieve airway inflammation and airway remodeling by decreasing IL-4 levels and increasing IL-12 levels.

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