中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 191-195.doi: 10.3969/j.issn.1673-8225.2011.02.001

• 骨组织构建 bone tissue construction •    下一篇

应用细胞片层技术构建组织工程骨

陈  涛1，王艳辉1，卜令学1，荆  恒1，高振华1，谭  帅1，王玲玲1，李宁毅2   

  1. 1青岛大学医学院附属医院医学影像科，山东省青岛市  266003
    2 青岛大学医学院口腔颌面外科，山东省青岛市  266003
  • 收稿日期:2010-10-08 修回日期:2011-01-10 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 通讯作者：李宁毅，博士，教授，青岛大学医学院口腔颌面外科，山东省青岛市 266003 ningyili342@163.com
  • 作者简介:陈涛★，男，1972年生,山东省商河县人，汉族，2001年青岛大学毕业，硕士，副教授，主要从事骨组织工程方面的研究。 Chentaojinxiang@163.com
  • 基金资助:

    国家自然科学基金资助项目(30872896)；山东省自然科学基金资助项目(Y2008C77)，课题名称：应用细胞片层技术构建血管化组织工程骨的实验研究。

Construction of tissue-engineered bone using cell sheet technology

Chen Tao1, Wang Yan-hui1, Bu Ling-xue1, Jing Heng1, Gao Zhen-hua1, Tan Shuai1, Wang Ling-ling1, Li Ning-yi2   

  1. 1Deparment of Medical Image, Affiliated Hospital of Qingdao University Medical College, Qingdao   266003, Shandong Province, China
    2Department of Oral and Maxillofacial Surgery, Qingdao University Medical College, Qingdao   266003, Shandong Province, China
  • Received:2010-10-08 Revised:2011-01-10 Online:2011-01-08 Published:2011-01-08
  • Contact: Li Ning-yi, Doctor, Professor, Department of Oral and Maxillofacial Surgery, Qingdao University Medical College, Qingdao 266003, Shandong Province, China ningyili342@163.com
  • About author:Chen Tao★, Master, Associate professor, Deparment of Medical Image, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China Chentaojinxiang@ 163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872896; the Natural Science Foundation of Shandong Province, No. Y2008C77

PDF

421

被引次数

8

摘要：

背景：传统的骨组织工程学在收获和转移细胞方面仍存在诸多不足，细胞片层技术是收获种子细胞和对种子细胞进行转移的一项新技术。
目的：应用细胞片层技术与传统骨组织工程相结合的方法构建组织工程骨。
方法：密度梯度离心法分离培养犬骨髓间充质干细胞，诱导其向成骨细胞分化。将诱导后的骨髓间充质干细胞接种至温度反应性培养皿中，先37 ℃饱和湿度培养，然后降温至20 ℃制备细胞片层；将犬脱钙骨基质/富血小板血浆/骨髓间充质干细胞细胞片层/骨髓间充质干细胞复合体植入犬左侧背阔肌下，术后4，8，12周，观察成骨情况。
结果与结论：当温度降至20 ℃时，骨髓间充质干细胞从温度反应性培养皿上完全脱落，形成细胞片层，将其覆盖于犬脱钙骨基质/富血小板血浆/骨髓间充质干细胞后植入犬背阔肌下，其成骨效果明显好于不加细胞片层对照侧。说明应用细胞片层技术与传统的骨组织工程方法相结合可构建出较理想的组织工程骨。

关键词: 细胞片层, 骨髓间充质干细胞, 温度反应性培养皿, 成骨细胞, 骨组织工程

Abstract:

BACKGROUND: Traditional bone tissue engineering faces shortcomings in harvesting or transferring cells. Cell sheet technology is a new skill for cell harvesting or transferring.
OBJECTIVE: To construct tissue-engineered bone using cell sheet technology and method of traditional bone tissue engineering.
METHODS: Canine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 ℃, 5% CO2 saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 ℃; demineralized bone matrix and platelet rich plasma (PRP) were prepared. The complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. The condition of osteogenesis was observed at 4, 8 and 12 weeks after operation.
RESULTS AND CONCLUSION: When temperature dropped at 20 ℃, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/BMSCs group. Cell sheet technology combined with traditional bone tissue provides a new way for construction of functional tissue-engineered bone.

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