中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7626-7630.doi: 10.3969/j.issn.1673-8225.2010.41.007

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

重组人骨形态发生蛋白2及制动因素对兔肌腱端重建的影响

张家红，何继业，王栋梁   

  1. 上海交通大学医学院附属新华医院骨科，上海市 200092
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 王栋梁，医学博士，副教授，主要从事肩肘创伤及骨病方面的研究。上海交通大学医学院附属新华医院骨科，上海市 200092 wang02_73@126.com
  • 作者简介:张家红★，男，1964年生，上海市人，汉族，在读硕士，副教授，主要从事创伤方面的研究。 zjh1964@126.com
  • 基金资助:

    上海市卫生局科研课题(2009063)，课题名称：应力及BMP对肌腱附着点结构重建的影响。

Influence of recombinant human bone morphogenetic protein-2 and immobilization on entheses reconstruction in rabbits

Zhang Jia-hong, He Ji-ye, Wang Dong-liang   

  1. Department of Orthopaedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai  200092, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Wang Dong-liang, Doctor, Associate professor, Department of Orthopaedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China Wang02_73@126.com
  • About author:Zhang Jia-hong★, Studying for master’s degree, Associate professor, Department of Orthopaedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China zjh1964@126.com
  • Supported by:

     the Science Foundation of Shanghai Health Bureau, No. 2009063*

PDF

332

被引次数

4

摘要：

背景：骨-肌腱结合结构的重建可能类似于软骨内成骨，只有形成坚固的止点，重建的韧带才能够真正发挥其生理功能。重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein-2，rhBMP-2)可使未分化间质细胞、成纤维细胞、成肌细胞及骨髓的基质细胞逆转分化为骨系细胞。
目的：观察rhBMP-2和制动因素对肌腱止点结构重建的影响。
方法：新西兰白兔建立肌腱止点结构重建模型后，随机分成3组。向rhBMP-2短期制动组和rhBMP-2长期制动组实验兔靠近胫骨隧道入口的屈趾总腱内注入重组人骨形态发生蛋白2溶液20 µL，模型组不予注射。术后rhBMP-2短期制动组术肢石膏固定1周后拆除；rhBMP-2长期制动组和模型组兔术肢全程石膏固定。各组分别于手术后2，4，8周通过免疫组化法和RT-PCR测定隧道入口部肌腱中的Ⅰ，Ⅱ型胶原、骨碱性磷酸酶和骨钙素的表达。
结果与结论：rhBMP-2可使肌腱止点结构重建兔Ⅰ型胶原、骨碱性磷酸酶和骨钙素表达水平随时间的延长而增加，而Ⅱ型胶原在术后增加至4周时表达至峰值，8周表达有所下降。注射rhBMP-2未对腱重建兔Ⅰ型胶原mRNA表达产生明显变化；而注射rhBMP-2可使Ⅱ型胶原、骨碱性磷酸酶和骨钙素mRNA表达显著增加(P < 0.05)，减少制动时间也能使Ⅱ型胶原、骨碱性磷酸酶和骨钙素mRNA表达显著增加(P < 0.05)。rhBMP-2能促进Ⅰ，Ⅱ型胶原、骨碱性磷酸酶和骨钙素的表达，并具有诱导肌腱内异位软骨成骨的作用，使肌腱端结构重建成为可能；长时间制动阻碍肌腱附着点的重建。

关键词: 肌腱止点, 重组人骨形态发生蛋白2, 胶原, 碱性磷酸酶, 骨钙素, 肌肉肌腱, 组织工程

Abstract:

BACKGROUND: Construction of bone-tendon junction is similar to endochondral ossification, and the constructed ligament would play its role only a direct tendon-to-bone enthesis regenerates. Recombinant human bone morphogenetic protein-2 (rhBMP-2) can induce undifferentiated mesenchymal cells, fibroblasts, myoblasts and bone marrow stromal cells differentiate into bonelines cells.   
OBJECTIVE: To evaluate the influence of the rhBMP-2 and post-operative immobilization on the entheses reconstruction in rabbits.
METHODS: New Zealand white rabbits were allocated averagely to 3 groups. Totally 20 µL rhBMP-2 solution was injected into the tendon at the point of the entry of bone tunnel in the rhBMP-2 short period immobilization and rhBMP-2 long period immobilization groups. Post-operatively, the operated limb was immobilized by plaster for 1 week in the rhBMP-2 short period immobilization group, and 8 weeks in rhBMP-2 long period immobilization and model groups. The expression of type Ⅰ, Ⅱ collagen, bone alkaline phosphatase (ALP) and osteocalcin (OCN) were examined by immnunohistochemistry and RT-PCR. 
RESULTS AND CONCLUSION: rhBMP-2 could increase the expressions of type Ⅰ collagen, bone ALP and osteocalcin with time prolonged. The expression of type Ⅱ collagen reached a peak at 4 weeks after operation, and then slightly decreased after 8 weeks. However, rhBMP-2 had no significant effect on type Ⅰ collagen mRNA expression, but increased the mRNA expression of type Ⅱ collagen, bone ALP and osteocalcin (P < 0.05), which could be obtained by reducing immobilization duration (P < 0.05). rhBMP-2 not only could upgrade the expression of type Ⅰ, Ⅱ collagen, bone ALP and osteocalcin, but also able to induce the ectopic endochondral ossification in tendon, thus, it makes possible that the entheses healed in the classic anatomical way. By the way, long period immobilization of operated limb has a negative effect on the entheses reconstruction.

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