中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6887-6890.doi: 10.3969/j.issn.1673-8225.2010.37.011

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

推拿?法样刺激对人脐静脉内皮细胞内钙离子浓度的影响

马惠昇1，张  宏2，严隽陶2，赵  谦2，牛  坤2   

  1. 1宁夏医科大学中医学院，宁夏回族自治区银川市 750004；2上海中医药大学附属岳阳中西医结合医院，上海市 200437
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 张宏，博士，副教授，上海中医药大学附属岳阳中西医结合医院，上海市 200437 zhanghdoctor@sina.com
  • 作者简介:马惠昇☆，男，1970年生，宁夏回族自治区贺兰县人，回族，2007年上海中医药大学毕业，博士，副教授，主要从事推拿手法基础与临床方面的研究。 madoctor@163.com
  • 基金资助:

    国家自然基金资助项目(30300462)“推拿?法操作动力学参数优化及行气活血效应机制研究”，上海市教育委员会科研创新项目(09YZ138)“不同人群推拿?法操作优化及舒筋活络效应机理研究”，上海市针灸推拿学重点学科建设项目(T0302-A24)“推拿?法舒筋活络效应的操作规范化及作用机理研究”.

Effects of stimulus like rolling manipulation on the intracellular calcium concentration in human umbilical vein endothelial cells

Ma Hui-sheng1, Zhang Hong2, Yan Jun-tao2, Zhao Qian2, Niu Kun2　   

  1. 1 Institute of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China; 2 Yueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai  200437, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Zhang Hong, Doctor, Associate professor, Yueyang Hospital of Integrated Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China 200437 zhanghdoctor@sina.com
  • About author:Ma Hui-sheng☆, Doctor, Asssociate professor, Institute of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China madoctor@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30300462*; Innovation Program of Shanghai Municipal Education Commission, No. 09YZ138*; Key Discipline Construction Project of Shanghai for Acupuncture and Massage, No. T0302-A24*

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493

被引次数

13

摘要：

背景：迄今对推拿?法作用机制的研究多着眼于软组织、骨关节、神经、血管等宏观层面，还需要从微观细胞学水平对手法力学刺激钙离子通道引发的效应机制进行研究。
目的：观察推拿?法样刺激对人脐静脉内皮细胞内钙离子浓度的影响。
方法：将正常培养和缺氧培养的人脐静脉内皮细胞均分为对照组、推拿组、维拉帕米组。对照组细胞不做任何处理，推拿组细胞用Fluo-3/AM标记后给予手法样压力刺激，压力大小为0.4 kg，频率120次/min，时间1 min；维拉帕米组细胞用Fluo-3/AM标记后，先加入终浓度为10-5 mol/L的维拉帕米，然后给与同样的?法样刺激。各组细胞在处理前后分别采用共聚焦激光扫描显微镜观察并测定细胞荧光强度进行比较。
结果与结论：手法样刺激和缺氧环境均可引起人脐静脉内皮细胞内游离钙离子浓度升高，但这两种促进人脐静脉内皮细胞内游离钙离子浓度升高的作用均能够被钙离子拮抗剂维拉帕米所抑制。由此推测，推拿?法的作用机制是通过机械刺激激活钙离子通道，引发细胞钙离子内流，促使细胞内游离钙离子浓度升高，进而启动并产生后续的生物学治疗效应。

关键词: 推拿?法, 钙离子, 人脐静脉内皮细胞, 信号转导, 组织工程

Abstract:

BACKGROUND: More researches of rolling manipulation in traditional Chinese Medicine focused on the macroscopic mechanism of action on soft tissues, joints, nerves, and blood vessels. But reports about its effect on cells such as the calcium ion channels are few.
OBJECTIVE: To observe the effect of rolling manipulation on the intracellular calcium concentration of human umbilical vein endothelial cells (HUVEC).
METHODS: The normal and hypoxic HUVEC were divided into the control, massage therapy, massage + verapamil group. The control group had no interference. Cells in the massage therapy group were marked with Fluo-3/AM, and then were stimulated with 0.4 kg in pressure, 120 times per minutes in frequency, and maintained for 1 minute. The massage + verapamil group cells were marked with Fluo-3/AM, and the final concentration of 10-5 mol/L of the verapamil were added in their culture fluid. They were stimulated as the massage therapy group cells. Each group cells were observed and measured the fluorescence intensity by the confocal laser scanning microscope before and after treatment.
RESULTS AND CONCLUSION: Both stimulus like rolling manipulation and hypoxic environment could increase the intracellular calcium ion concentration, but they can be prevented by verapamil. Accordingly, it is presume that the mechanism of action of rolling manipulation is activating Ca 2+ channels, causing Ca 2+ internal flow and then increasing the intracellular Ca 2+ , which primings its therapeutic effects.

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