中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (37): 6869-6872.doi: 10.3969/j.issn.1673-8225.2010.37.007

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

组织工程皮肤移植过程中白细胞介素10和肿瘤坏死因子α的表达

彭  燕1，刘德伍1，杨  慧2，蓝  蔚1，钟清玲1   

  1. 1南昌大学第一附属医院烧伤研究所，江西省南昌市 330006；2南昌大学医学院免疫教研室，江西省南昌市  330006
  • 出版日期:2010-09-10 发布日期:2010-09-10
  • 通讯作者: 刘德伍，博士，教授，主任医师，博士生导师，主要从事创面修复和皮肤组织工程研究。南昌大学第一附属医院烧伤研究所，江西省南昌市 330006 dewuliu@126.com
  • 作者简介:彭燕★，女，1975年生，江西省永修县人，汉族，硕士，主管技师，主要从事创面修复和皮肤组织工程研究。
  • 基金资助:

    江西省卫生厅科技计划项目(20093037)“新型组织工程皮肤的构建与免疫原性研究”、南昌大学科技基金项目(2009036)“含表皮干细胞的组织工程皮肤免疫原性研究”。

Expression of interleukin 10 and tumor necrosis factor alpha in tissue engineered skins transplantation

Peng Yan1, Liu De-wu1, Yang Hui2, Lan Wei1, Zhong Qing-ling1   

  1. 1 Institute of Burn, First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China; 2 Department of Immunology, Medical College of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Liu De-wu, Doctor, Professor, Chief physician, Doctoral supervisor, Institute of Burn, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China dewuliu@126.com
  • About author:Peng Yan★, Master, Technician-in-charge, Institute of Burn, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Supported by:

    the Science and Technology Planning Project of Health Bureau of Jiangxi Province, No. 20093037*; the Science and Technology Foundation of Nanchang University, No. 2009036*

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摘要：

背景：白细胞介素10和肿瘤坏死因子α在异体异种皮肤移植局部免疫与排斥反应中发挥了重要作用。但在组织工程人工皮肤移植过程中局部皮肤组织内白细胞介素10和肿瘤坏死因子α的表达情况目前尚不清楚。
目的：采用表皮干细胞联合脱细胞真皮构建人工皮肤并移植修复兔全层皮肤缺损创面，观察创面修复效果和局部皮肤组织白介素10与肿瘤坏死因子α的表达变化。
方法：将体外培养的人表皮干细胞或角质细胞接种到脱细胞真皮支架中，构建组织工程人工皮肤；取新西兰白兔常规制作背部全层皮肤缺损创面随机分为4组，表皮干细胞组、角质细胞组用含表皮干细胞或角质细胞的组织工程皮肤移植于皮肤缺损创面；脱细胞真皮组移植单纯脱细胞真皮；对照组创面空置。观察创面修复情况，局部炎症反应，创面愈合时间。各组分别于术后7d在部分创面取材观察组织形态和白细胞介素10与肿瘤坏死因子α表达。
结果与结论：含表皮干细胞的人工皮肤移植后创面愈合良好，局部炎症反应轻微，无出血、积脓、坏死，创面愈合时间较角质细胞组明显缩短。白细胞介素10在各组均有表达，其中表皮干细胞组表达最强，角质细胞组次之，脱细胞真皮组和对照组最弱。肿瘤坏死因子α在各组也均有表达，其中角质细胞组表达最强，表皮干细胞组次之，脱细胞真皮组和对照组较弱。说明以表皮干细胞作为种子细胞联合脱细胞真皮构建人工皮肤可用有效促进皮肤缺损创面的修复治疗，创面修复过程中局部皮肤组织内白细胞介素10和肿瘤坏死因子的表达变化可能是其取得较好效果的主要机制之一。

关键词: 表皮干细胞, 组织工程, 皮肤移植, 肿瘤坏死因子&alpha, 白细胞介素10

Abstract:

BACKGROUND: Interleukin 10 (IL-10) and tumor necrosis factor α (TNF-α) play an important role in allogeneic and xenogeneic skin graft rejection in local immune. The expression of TNF-α and IL-10 is unclear in the process of artificial skin graft in local skin tissue.

OBJECTIVE: To construct the artificial skin with epidermal stem cells and acellular dermal and to repair the rabbit full thickness dermal wounds, to observe the effect of wound repair and local skin tissue IL-10 and TNF-α expression.
METHODS: The in vitro cultured human epidermal stem cells or keratinocytes were incubated into acellular dermal matrix to construct tissue-engineered artificial skin. New Zealand White rabbits were prepared for back full thickness dermal wounds and randomly divided into 4 groups. Tissue-engineered skin grafts with epidermal stem cells, keratinocytes or acellular dermis were used to repair skin wounds. There was no treatment in the control group. The wound healing, local inflammatory response, and wound healing time were observed. The histomorphology of wound surface and the expressions of TNF-α and IL-10 at 7 days after operation were observed. 
RESULTS AND CONCLUSION: In the epidermal stem cell group, the local inflammatory response was slightly, without bleeding, empyema or necrosis. The wound healing time was shortened significantly compared with than of the keratinocyte group. There was IL-10 expression in all groups, especially strongest in the epidermal stem cell group, followed by keratinocyte group; the expression was weak in the acellular dermis and control groups. The TNF-α was expressed in all groups, which was strongest in the keratinocyte group, followed by epidermal stem cell group, but weak in the acellular dermis and control groups. The construction of the artificial skin with epidermal stem cells and acellular dermal can be used effectively to promote the repair of skin defect wound treatment. The expression changes of IL-10 and TNF-α in local skin tissue maybe one of its main mechanisms.

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