中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (36): 6677-6680.doi: 10.3969/j.issn.1673-8225.2010.36.006

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

人骨髓间充质干细胞诱导向多巴胺能神经元的分化

金  辉，张  蕾，李伟伟   

  1. 辽宁医学院，辽宁省锦州市  121001
  • 出版日期:2010-09-03 发布日期:2010-09-03
  • 通讯作者: 张蕾，博士，硕士生导师，辽宁医学院，辽宁省锦州市 121001
  • 作者简介:金辉★，女，1971年生，辽宁省锦州市人，满族，辽宁医学院在读硕士，主管检验师，主要从事干细胞研究。dongzl1@sina. com

Induced differentiation of human bone marrow mesenchymal stem cells into dopaminergic neurons

Jin Hui, Zhang Lei, Li Wei-wei   

  1. Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Zhang Lei, Doctor, Master’s supervisor, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • About author:Jin Hui★, Studying for master’s degree, Laboratorian-in-charge, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China dongzl1@sina.com

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308

被引次数

8

摘要：

背景：体内外研究发现人骨髓间充质干细胞分化为神经元的比率都明显低于胶质细胞，并且这为数不多的神经元会逐渐死亡，而最终存活的细胞中神经元的数量更少。
目的：观察人骨髓间充质干细胞体外诱导分化为多巴胺能神经元的潜能。
方法：分离纯化和扩增人骨髓间充质干细胞，在体外先用碱性成纤维细胞生长因子和表皮细胞生长因子进行预诱导后，以胶质细胞源性神经营养因子和血管紧张素Ⅱ联合诱导人骨髓间充质干细胞向神经元和多巴胺能神经元分化。观察分化过程中细胞的形态变化，利用免疫组织化学检测神经元和多巴胺能神经元特异性标志物的表达情况。
结果与结论：人骨髓间充质干细胞经诱导后的细胞呈现双极、多极和锥形的典型神经元细胞的形态，明显表达抗人神经巢蛋白[(55.7±4.3)%]和神经元特异性烯醇化酶[(78.2±6.7)%]，而且大部分人骨髓间充质干细胞表达酪氨酸羟化酶[(48.5±5.6)%]，不表达神经胶质细胞标记物胶质纤维酸性蛋白。提示在适宜的条件下，人骨髓间充质干细胞可分化成神经元和多巴胺能神经元样细胞。

关键词: 神经元样细胞, 人骨髓间充质干细胞, 诱导分化, 多巴胺能神经元样细胞, 干细胞培养与分化

Abstract:

BACKGROUND: In vivo and vitro studies have confirmed that the ratio of differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into neurons was obviously lower compared with glial cells. Moreover, these neurons will gradually die. Finally, the number of neurons in living cells is low.
OBJECTIVE: To explore the potential of hBMSCs differentiation into dopaminergic neuron-like cells in vitro. 
METHODS: The hBMSCs were isolated, purified and amplified. After pretreatment with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) in vitro, the hBMSCs were cultured at the medium containing glial cell line-derived neurotrophic factor (GDNF) and Angiotensin II (AngⅡ) to induce hBMSCs differentiation into neurons and dopaminergic neurons. Changes in cell morphology were observed during differentiation. Marker expression of neurons and dopaminergic neurons were detected by immunohistochemistry.
RESULTS AND CONCLUSION: Following induction, hBMSCs presented dipolar, multipolar and pyramidal typical neuron morphology, significantly expressed anti-human nerve nestin [(55.7±4.3)%] and neuron specific enolase [(78.2±6.7)%]. Moreover, a majority of hBMSCs expressed tyrosine hydroxylase [(48.5±5.6)%], but did not express glial fibrillary acidic protein. These indicated that under suitable conditions, hBMSCs can differentiate into neurons and dopaminergic neuron-like cells.

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