中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (34): 6359-6362.doi: 10.3969/j.issn.1673-8225.2010.34.021

• 药物控释材料 drug delivery materials • 上一篇    下一篇

功能基化天然生物多糖吸附剂制备及对血浆胆红素的清除

王  勇1，陆  伟2，张政朴3，吴伟华3   

  1. 1天津市海河医院，天津市  300350; 2天津市传染病医院，天津市 300192；3 天津南开大学高分子研究所，天津市   300071
  • 出版日期:2010-08-20 发布日期:2010-08-20
  • 通讯作者: 陆伟，博士，主任医师，天津市传染病医院，天津市 300192 luwei1966@126.com
  • 作者简介:王勇，男，1959年生，天津市人，汉族，1982年南开大学毕业，高级工程师，主要从事医用高分子材料研究。 Tjs.hhyywy@yahoo.com.cn
  • 基金资助:

    天津市应用基础与前沿技术研究计划 (08JCYBJC11300)。

Pareparation of functional group natural biological polysaccharide adsorbent and removal of plasma bilirubin

Wang Yong1, Lu Wei2, Zhang Zheng-pu3, Wu Wei-hua3   

  1. 1 Tianjin Haihe Hospital, Tianjin   300350, China; 2 Tianjin Infectious Diseases Hospital, Tianjin   300192, China; 3 Polymer Research Institute of Nankai University, Tianjin   300071, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Lu Wei, Doctor, Chief physician, Tianjin Infectious Diseases Hospital, Tianjin 300192, China luwei1966@126.com
  • About author:Wang Yong, Senior engineer, Tianjin Haihe Hospital, Tianjin 300350, China Tjs.hhyywy@yahoo.com.cn
  • Supported by:

    Applied Basic and Advanced Research Program of Tianjin, No. 08JCYBJC11300*

PDF

618

被引次数

11

摘要：

背景：目前临床上应用的血液/血浆灌流吸附剂主要为活性炭和化学合成高分子大孔树脂。活性炭微末会造成栓塞，树脂致孔剂多为甲苯类溶剂不易洗脱干净引起不良反应。用天然高分子材料修饰交联制备吸附剂意义重大。
目的：用天然生物多糖-壳聚糖作为胆红素吸附剂的骨架基质，经胺化修饰交联，以蔗糖为致孔剂制备功能基化交联壳聚糖吸附剂，比较该吸附剂与市售BL-300型血浆灌流吸附剂对血浆胆红素吸附性能。
方法：用功能基化交联壳聚糖吸附剂和市售BL-300型血浆灌流吸附剂，分别对高胆红素血浆进行灌流吸附试验，全自动生化分析仪测定不同吸附剂对血浆胆红素的吸附率。
结果与结论：功能基化交联壳聚糖吸附剂和市售BL-300吸附剂对不同浓度的胆红素血浆均具有良好的吸附效果，功能基化交联壳聚糖吸附剂对胆红素的吸附率可达50.2%，BL-300吸附剂对胆红素的吸附率可达56.8%，与文献报道基本一致。结果证实，功能基化交联壳聚糖吸附剂血浆灌流清除胆红素效果明显且安全可靠，有望作为生物医用血浆灌流材料。

关键词: 壳聚糖, 胆红素吸附, 血浆灌流, 生物材料, 高分子材料

Abstract:

BACKGROUND: Current blood/plasma perfusion adsorbents applied in clinics are mainly activated carbon and chemically synthetized macroporous polymer. Carbon window dressing will cause embolism, and resin porogen, most of which is solvent toluene, may be difficult to clean and induce adverse reactions. Natural polymer with amine modified by cross-linking plays a pivotal role in preparation of the adsorbent.
OBJECTIVE: To prepare functional group crosslinked chitosan adsorbent with natural biological polysaccharide-chitosan as a skeletal matrix of bilirubin adsorbent, modified by amination crosslinking, and with sucrose as porogen, to compare with the commercially available BL-300 plasma perfusion adsorbents on the plasma bilirubin adsorption.
METHODS: Functional group crosslinked chitosan adsorbents and commercial BL-300 type plasma perfusion adsorbent were utilized, hyperbilirubinemia plasma was carried out on plasma perfusion tests, plasma bilirubin adsorption rate of different adsorbents was determined using automatic biochemical analyzer.
RESULTS AND CONCLUSION: The functional group crosslinked chitosan adsorbent and commercial BL-300 adsorbent showed good adsorption on plasma bilirubin at various concentrations, the adsorption rate was 50.2% in functional group crosslinked chitosan adsorbent, and 56.8% in BL-300 adsorbent, which was consistent with previous reports. Results showed that the functional group crosslinked chitosan adsorbent is effective, safe and reliable to scavenge bilirubin.

中图分类号: