中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (24): 4411-4415.doi: 10.3969/j.issn.1673-8225.2010.24.010

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

冷保存方法构建的大鼠肝移植模型

余  强，史宪杰，李海林，董家鸿   

  1. 解放军总医院肝胆外科，北京市  100853
  • 出版日期:2010-06-11 发布日期:2010-06-11
  • 通讯作者: 董家鸿，主任医师，博士生导师，解放军总医院肝胆外科，北京市 100853 dongjh301@163.com
  • 作者简介:余 强，男，1980年生，北京人，汉族，2003年第四军医大学毕业，医师，主要从事肝胆外科及肝移植方面的研究。

A rat model of liver transplantation constructed by cryopreservation   

Yu Qiang, Shi Xian-jie, Li Hai-lin, Dong Jia-hong   

  1. Department of Hepatobiliary Surgery General Hospital of Chinese PLA, Beijing  100853, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Dong Jia-hong, Chief physician, Doctoral supervisor, Department of Hepatobiliary Surgery, General Hospital of Chinese PLA, Beijing 100853, China dongjh301@163.com
  • About author:Yu Qiang, Physician, Department of Hepatobiliary Surgery, General Hospital of Chinese PLA, Beijing 100853, China

PDF

347

被引次数

7

摘要：

背景：肝移植是终末期肝病最为有效的治疗手段，移植后胆病是制约肝移植发展的主要障碍之一。
目的：建立稳定的大鼠冷保存肝移植模型，探讨冷保存对肝移植后肝脏胆管的影响。
方法：120只雄性SD大鼠随机分为4组，按照组内随机配对的原则，体质量相对较轻的大鼠做为供体，供肝置于4 ℃ UW液中分别保存2，8，16 h后行原位肝移植。在“两套袖法”基础上，以“支架法”建立动脉化大鼠原位肝移植模型，供受体肝总动脉采用改良“支架法”进行端端吻合，重建肝动脉血供。记录移植手术时间及移植成功率，并分别于移植后3， 7 d检测血清转氨酶、总胆红素、碱性磷酸酶水平，同时观察组织病理学改变。
结果与结论：实验共完成55例次大鼠原位肝移植手术，手术成功率为93%。冷保存2，8，16 h组术后7 d存活率分别为100%(9/9)，83%(10/12)，73%(8/11)。随着冷保存时间的延长，肝功能及肝内胆管损伤加重，胆管组织病理学评分显示各组间差异有显著性意义(P < 0.05)。其中供肝冷保存16 h大鼠肝移植模型既有较高的手术存活率又有严重的胆管损伤，是研究冷保存对肝移植胆管病影响的较好模型。

关键词: 肝移植, 冷保存, 动物模型, 大鼠, 肝脏组织工程

Abstract:

BACKGROUND: Liver transplantation is the most effective treating method for end-stage liver disease. Biliary lesion following transplantation is one of primary problems for liver transplantation.
OBJECTIVE: To develop a model of liver transplantation in rats to study the effect of cryopreservation time on bile ducts following liver transplantation.
METHODS: A total of 120 healthy, male, SD rats. According to the random pair principle, rats with smaller weight served as donors. The donor livers were preserved at UW solution at 4 ℃ for 2, 8, 16 hours and transplanted to the recipients. Based on the classic “two cuff” technique for orthotropic liver transplantation, end-to-end anastomosis between the common hepatic artery of donor and recipient by the modified “Gao stent” was performed to reconstruct hepatic artery blood flow. The operative duration and successful transplantation ratio were recorded. The levels of serum alamine aminotransferase, total bilirubin, and alkaline phosphatase were determined at 3 and 7 days after transplantation. At the same time, the histopathological changes were observed. 
RESULTS AND CONCLUSION: In total 55 rats were performed orthotopic liver transplantation, and the successful transplantation ratio was 93%. The success ratio of 2-, 8- and 16-hour group was 100% (9/9), 83% (10/12) and 73% (8/11), respectively. Prolonged cryopreservation time can cause the severe bile duct injury. Bile duct histopathology score showed differences between groups were significant (P < 0.05). The model in rats with grafts undergoing 16-hour cryopreservation provides suitable model for studying the effects of cryopreservation on bile ducts after liver transplantation.

中图分类号: