中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4257-4261.doi: 10.3969/j.issn.1673-8225.2010.23.018

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞移植修复大鼠慢性胰腺损伤

刘洪斌，杨  静，李东华，王  蒨   

  1. 天津医科大学南开临床学院，天津市 300100
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 作者简介:刘洪斌，男，1973年生，河北省曲阳县人，汉族，1996年天津医科大学毕业，博士，副研究员，主要从事肝胆胰疾病的基础研究。 jtsstj1@yahoo. com.cn

Transplantation of bone marrow mesenchymal stem cells for repair of chronic pancreatic injury in rats

Liu Hong-bin, Yang Jing, Li Dong-hua, Wang Qian   

  1. Nankai Clinical College, Tianjin Medical University, Tianjin 300100, China
  • Online:2010-06-04 Published:2010-06-04
  • About author:Liu Hong-bin, Doctor, Associate investigator, Nankai Clinical College, Tianjin Medical University, Tianjin 300100, China jtsstj1@yahoo.com. cn

PDF

511

被引次数

24

摘要：

背景：慢性胰腺炎是以胰腺慢性炎症损伤及纤维化为主要病理特征的慢性进展性疾病，骨髓间充质干细胞可能在其治疗中具有潜在价值，但目前这一领域的研究尚不多见。
目的：观察骨髓间充质干细胞对慢性胰腺炎大鼠胰腺损伤的修复作用并探讨其治疗机制。
方法：体外培养、扩增大鼠骨髓间充质干细胞并对其进行鉴定；采用胆胰管逆行注射油酸法制备Wistar大鼠慢性胰腺炎模型，随机数字表法分为假手术组、模型组、间充质干细胞治疗组。间充质干细胞治疗组造模后20 d经尾静脉注射间充质干细胞生理盐水细胞悬液1 mL(含细胞3×106)，第40天和第60天各重复1次；模型组经尾静脉注射等体积生理盐水；假手术组不进行十二指肠穿刺及胆胰管逆行注射，其余操作同模型组。3组动物均于治疗结束后取胰腺组织行病理组织学检查；并检测胰腺组织内结缔组织生长因子、转化生长因子β、Ⅰ、Ⅲ型胶原水平及髓过氧化物酶活性。
结果与结论：经间充质干细胞治疗的慢性胰腺炎大鼠胰腺病变程度及纤维化程度显著减轻；胰腺组织内结缔组织生长因子、转化生长因子β、Ⅰ、Ⅲ型胶原水平及髓过氧化物酶活性显著降低(P < 0.01)。提示骨髓间充质干细胞对慢性胰腺炎大鼠胰腺损伤有显著的修复作用，其机制可能和抑制结缔组织生长因子、转化生长因子β产生、抑制炎症反应、减少胶原增生有关。

关键词: 慢性胰腺炎, 骨髓间充质干细胞, 纤维化, 结缔组织生长因子, 转化生长因子&beta

Abstract:

BACKGROUND: Chronic pancreatitis is a chronic necroinflammatory process characterized pathologically by chronic inflammation and fibrosis in pancreas tissue. Bone marrow mesenchymal stem cells (BMSCs) maybe play a potential role in treating chronic pancreatitis. However, few reports have addressed the use of MSCs in pancreatic regeneration.
OBJECTIVE: To investigate the effects and mechanisms of BMSCs for treating chronic pancreatitis in rats.
METHODS: BMSCs were isolated, cultured and identified in vitro. Chronic pancratitis rat model was induced by infusion of oleic acid to bile-pancreas duct in Wistar rats. The Wistar rats were assigned randomly to sham operation group, model group and BMSCs group. BMSCs were transplanted to the rats from BMSCs group through caudal vein injection in the number of 3 ×    106 /mL at 20 days after the model induction and the injection was repeated twice at 40 days and 60 days after model induction. For the model group, the equal volume of saline was injected into the caudal vein. For the sham operation group, duodenum puncture and infusion of oleic acid to bile-pancreas duct were not done. For all the rats, pancreatic tissues were collected and underwent histopathological examination. Pancreatic connective tissue growth factor (CTGF), transforming growth factor β (TGF-β), type I collagen, type III collagen and myeloperoxidase (MPO) activity were detcted.
RESULTS AND CONCLUSION: The pathological injury and the fibrosis in the BMSCs group were ameliorated signicantly. The contents of pancreatic CTGF, TGF-β, type I collagen, type III collagen and MPO were all decreased significantly (P < 0.01). These results suggested that BMSCs have obvious repairing effects on the injured pancreatic tissue of chronic pancreatitis rats, which may be related to the inhibition of CTGF, TGF-β release, decreased production of type I, type III collagen, and inhibition of inflammatory reaction.

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