中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (21): 3832-3838.doi: 10.3969/j.issn.1673-8225.2010.21.008

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

壳聚糖-肝素静电自组装多层膜在钛表面进行的生物化修饰

舒  瑶1，李全利2，欧国敏3，宫  苹3，邹敬才1   

  1. 1解放军军事医学科学院附属医院口腔科，北京市 100070；2安徽医科大学口腔医学院，安徽省合肥市 230032；3四川大学华西口腔医学院，四川省成都市  610041
  • 出版日期:2010-05-21 发布日期:2010-05-21
  • 通讯作者: 欧国敏，博士，副教授，四川大学华西口腔医学院，四川省成都市 610041 guominou66@yahoo.com
  • 作者简介:舒 瑶★，女，1983年生，四川省大竹县人，汉族，2008年四川大学华西口腔医学院毕业，硕士，医师，主要从事口腔种植材料的相关研究。 lanzhushuyu@yahoo.com.cn
  • 基金资助:

    安徽省高等学校自然科学基金重点项目(KJ2009A169)；安徽省科技攻关项目(08010302196)。

Titanium surface modification with chitosan-heparin multilayers using electrostatic self-assebly technique

Shu Yao1, Li Quan-li2,Ou Guo-min3, Gong Ping3, Zou Jing-cai1   

  1. 1 Department of Stomatology, Affiliated Hospital of the Academy of Military Medical Sciences of Chinese PLA, Beijing 100071, China; 2 College of Stomatology, Anhui Medical University, Hefei  230032, Anhui Province, China; 3 Department of Prosthodontics, West China College of Stomatology, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Li Quan-li, Doctor, Associate professor, College of Stomatology, Anhui Medical University, Hefei 230032, Anhui Province, China ql-li@126.com
  • About author:Shu Yao★, Master, Physician, Department of Stomatology, Affiliated Hospital of the Academy of Military Medical Sciences of Chinese PLA, Beijing 100071, China lanzhushuyu@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Anhui Higher Educootion Institutions, No. KJ2009A169*; Scientific and Technological Program of Anhui Province, No. 08010302196*

PDF

631

被引次数

16

摘要：

背景：基于聚电解质阴阳离子交替组装的静电自组装技术可在温和、简单、易控的条件下实现多种生物大分子在材料表面的固定，已成为生物材料表面设计的重要手段。
目的：利用静电自组装技术将具有生物活性的壳聚糖和肝素固定在钛表面，实现钛表面的氨基多糖生物化修饰，构建一种钛种植体材料的新型生物化表面，以改善钛的细胞相容性。
方法：首先采用NaOH处理钛基材，获得多孔、负电荷的钛表面；然后吸附一层正电荷的聚赖氨酸；最后，多次交替吸附负电荷的肝素和正电荷的壳聚糖，形成以壳聚糖为最外层的多层膜结构。通过漫反射红外光谱扫描电镜和原子力显微镜对多层膜进行表征。并与成骨细胞共培养，观察成骨细胞的黏附、增殖以及分化情况。
结果与结论：红外光谱、原子力显微镜、扫描电镜结果表明肝素-壳聚糖多层膜逐渐形成。此涂层可促进成骨细胞的黏附、增殖和分化。肝素-壳聚糖多层膜有望成为一种新型的生物化钛表面，从而改善钛表面的生物相容性。

关键词: 静电自组装, 钛, 表面改性, 壳聚糖, 肝素

Abstract:

BACKGROUND: Based on the consecutive adsorption of polyanions and polycations via electrostatic interactions, electrostatic self-assebly (ESA) technique has emerged as a versatile, inexpensive yet efficient technique to “build” biologically active surfaces for modification of biomaterial surface.
OBJECTIVE: Bioactive chitosan and heparin multilayers fixed on titanium surface via ESA technique are expected to be a new biologic surface to improve the cell biocompatibility of titanium.
METHODS: The film growth was initialized by depositing one layer of positively charged poly-L-lysine (PLL) on the NaOH-treated titanium substrate (negatively charged surface). Then, the film was formed by the alternate deposition of negatively charged heparin (Hep) and positively charged chitosan (Chi) via electrostatic interactions of polyelectrolytes, and terminated with an outermost layer of Chi. The chemical composition, surface topography as well as roughness were investigated by using diffuse reflectance Fourier transform infrared spectroscopy (DR-FTIR), scanning electric microscope (SEM) and atomic force microscope (AFM), respectively. We cultured osteoblasts on the surface of modified-titanium (Ti/Chi) and pure Ti (cpTi) respectively in vitro to observe morphologic change of cell attachment and spreading by SEM, and to estimate cell proliferation and differentiation by MTT test and ALP activity analysis.
RESULTS AND CONCLUSION: The analysis of DR-FTIR, SEM, and AFM confirmed that Chi-Hep multilayers formed gradually on the titanium surface. The film could improve the adherence, proliferation and differentiation of osteoblast. These results may provide a basis for the preparation of titanium surface modification for dental implants.

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