中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (20): 3726-3729.doi: 10.3969/j.issn.1673-8225.2010.20.027

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

周围神经损伤后最佳修复时间时机的选择

张大鹏1，潘世奇1，侯明明1，杨 琪1，王延忠2   

  1. 1哈尔滨医科大学附属第四医院骨科，黑龙江省哈尔滨市  150001；  
    2勃利县人民医院骨科，黑龙江省勃利县  154500
  • 出版日期:2010-05-14 发布日期:2010-05-14
  • 通讯作者: 潘世奇，主任医师，哈尔滨医科大学附属第四医院骨科，黑龙江省哈尔滨市 150001 zdap2006@ yahoo.com.cn
  • 作者简介:张大鹏，男，1973年生，黑龙江省哈尔滨市人，汉族，1999年哈尔滨医科大学毕业，硕士，主治医师，主要从事周围血管损伤修复、促周围神经再生的研究。
  • 基金资助:

    黑龙江省卫生厅课题(2007-442)

Optimal time for repairing peripheral nerve defects following injury

Zhang Da-peng1, Pan Shi-qi1, Hou Ming-ming1, Yang Qi1, Wang Yan-zhong2   

  1. 1Department of Orthopaedics, the Fourth Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China;
    2Department of Orthopaedics, Boli People’s Hospital, Boli  154500, Heilongjiang Province, China
  • Online:2010-05-14 Published:2010-05-14
  • Contact: Pan Shi-qi, Chief physician, Department of Orthopaedics, the Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China zdap2006@yahoo.com.cn
  • About author:Zhang Da-peng, Master, Attending physician, Department of Orthopaedics, the Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
  • Supported by:

    the Subject of Health Department of Heilongjiang Province, No. 2007-442*

PDF

464

被引次数

25

摘要：

背景：关于周围神经损伤的修复机制研究报道较多，但对周围神经损伤修复时间的选择的研究较少。

目的：通过观察大白兔周围神经损伤后不同时间修复的效果，以选择周围神经损伤后进行修复的最佳时机。

方法：随机将大白兔分为4组。剪下长约1 cm的坐骨神经制作周围神经损伤动物模型。即时修复组即时进行神经修复组；2，4周和3个月修复组神经两断端分别固定于肌膜上，缝合伤口，分别在2，4周后和3个月后修复坐骨神经。术后3个月于缝合神经处的取材，检测运动神经传导速度，苏木精-伊红染色行病理学观察，电镜观察移植神经段结构，并进行神经移植体轴突计数。

结果与结论：2周修复组大白兔运动神经传导速度明显快于4周和3个月修复组(P < 0.01)，与即时修复组比较差异无显著性意义(P > 0.05)。2周修复组神经移植处组织形态、结构明显好于4周和3个月修复组。2周修复组神经移植体轴突计数明显多于4周修复和3个月修复组(P < 0.01)。结果提示在2周后修复神经损伤优于其他时间点，是周围神经损伤后进行修复的最佳时机。

关键词: 周围神经损伤, 不同时间, 修复, 兔, 神经组织工程

Abstract:

BACKGROUND: Most studies regarding peripheral nerve injury repair focus on repair mechanisms rather than selection of repair times. 

OBJECTIVE: To observe the different time points on peripheral nerve injury repair and to select an optimal time.

METHODS: White rabbits were prepared for peripheral nerve injury models and randomly divided into 4 groups. In the immediate restoration group, the injured nerves were repaired immediately. In the other groups, the broken ends were fixed on sarcolemma and the sciatic nerve was repaired at 2, 4 weeks and 3 months after injury. The motor nerve conduction velocity, pathological observation, and structure of transplanted nerve segment were observed at 3 months after operation. The number of axon was also counted.

RESULTS AND CONCLUSION: Compared with 4-week and 3-month restoration groups, the motor nerve conduction velocity of the 2-week restoration group was obviously faster (P < 0.01), but the difference was significant compared with the immediate restoration group (P > 0.05). The histomorphology and structure of the 2-week restoration group were better than those of the 4-week and 3-month restoration groups. In addition, the number of axon in the 2-week restoration group was greater than those of the 4-week and 3-month restoration groups (P < 0.01). All results demonstrated that the outcome of 2-week restoration is superior to other time points, which is an optimal time for peripheral nerve injury.

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