中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (8): 1513-1516.doi: 10.3969/j.issn.1673-8225.2010.08.044

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

骨修复用纳米羟基磷灰石/聚酰胺66的体内外生物相容性

杨  萌1，陈民芳1，王  昊2，李长义3   

  1. 1天津理工大学材料科学与工程学院，天津市  300384；2天津武警医学院附属医院，天津市  300162； 3天津医科大学口腔医院，天津市  300070
  • 出版日期:2010-02-19 发布日期:2010-02-19
  • 通讯作者: 陈民芳，博士，教授，天津理工大学材料科学与工程学院，天津市 300384
  • 作者简介:杨 萌★，女，1984年生，天津市人，汉族，天津理工大学在读硕士，主要从事生物材料方面的研究。
  • 基金资助:

    国家自然科学基金项目(50572068)；天津市自然科学基金重点项目(09JCZDJC18500)。

In vitro and in vivo biocompatibility of nano-hydroxyapatite/polyamide 66 as bone repair material

Yang Meng1, Chen Min-fang1, Wang Hao2, Li Chang-yi 3   

  1. 1 School of Materials Science and Engineering, Tianjin University of Technology, Tianjin   300384, China; 2 Affiliate Hospital of Medical College of  the Chinese People’s Armed Police Forces, Tianjin  300162, China; 3 Stomatological Hospital of Tianjin Medical University, Tianjin   300070, China
  • Online:2010-02-19 Published:2010-02-19
  • Contact: Chen Min-fang, Doctor, Professor, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, China mfchentj@126.com
  • About author:Yang Meng★, Studying for master’s degree, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, China yangmeng84@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 50572068*; the Key Project of Natural Science Foundation of Tianjin City, No. 09JCZDJC18500*

PDF

445

被引次数

24

摘要：

背景：采用基于纳米羟基磷灰石溶胶新方法制备纳米羟基磷灰石/聚酰胺66复合材料，该材料提高了纳米羟基磷灰石在聚酰胺66基体中的均匀分布和二者的有效键合，进而有利于改善材料的生物性能，有望成为新型骨修复材料。
目的：评价纳米羟基磷灰石/聚酰胺66复合材料体内外生物相容性。
方法： ①将原代培养的成骨细胞与纳米羟基磷灰石/聚酰胺66及聚酰胺66材料复合培养，使用倒置相差显微镜和场发射扫描电子显微镜观察材料周围及表面的细胞形态。②将纳米羟基磷灰石/聚酰胺66复合材料植入兔右侧胫骨，将聚酰胺66作为对照组材料植入兔左侧胫骨。在术后2，8周，取材料周围骨组织进行病理组织切片观察。
结果与结论： ①纳米羟基磷灰石/聚酰胺66和聚酰胺66未表现出明显的细胞毒性，纳米羟基磷灰石/聚酰胺66材料周围细胞形态好于聚酰胺66，且纳米羟基磷灰石/聚酰胺66表面细胞数量多于聚酰胺66，在复合培养的第3天差异尤其显著(P < 0.01)。②在植入早期，与纳米羟基磷灰石/聚酰胺66相接的骨组织成骨细胞活跃且该组材料周围的骨形成过程较对照组更快。结果说明纳米羟基磷灰石/聚酰胺66复合材料较聚酰胺66有更好的生物相容性。

关键词: 纳米羟基磷灰石/聚酰胺66, 聚酰胺66, 生物相容性, 细胞培养, 骨修复材料

Abstract:

BACKGROUND: Nanohydroxyapatite reinforced polyamide 66 (n-HA/PA66) prepared using the novel process based on nanohydroxyapatite (n-HA) sol may promote the biocompatibility due to the well distribution of n-HA in the polyamide66 (PA66) matrix and chemical bond at organic-inorganic interface. n-HA/PA66 composite has been proposed as a promising bone repair biomaterial.
OBJECTIVE: To investigate the biocompatibility of the novel material both in vitro and in vivo.
METHODS: Primarily cultured osteoblasts were co-cultured with n-HA/PA66 and PA66. The cell attachment and morphology were studied using phase contrast light microscope (PCLM) and field-emission scanning electron microscope (FE-SEM). Moreover, n-HA/PA66 pins, with PA66 pins as control, were implanted into the right and left (control group) shinbone shafts of the rabbits, respectively. Materials were harvested at weeks 2, 8 and tissue sections were observed.
RESULTS AND CONCLUSION: n-HA/PA66 and PA66 exhibited no cytotoxicity with osteoblasts, while the cellular morphology near the samples was better in the test group than in the control one. Moreover, the cell density attached on n-HA/PA66 was higher than that on PA66 and the cell number were especially different after co-culturing for 3 days (P < 0.01). The osteoblasts at the interface between the host bone and n-HA/PA66 also had a higher activity during the earlier period after implantation. Meanwhile the bone formation process was faster and effective in the experimental group. Results suggested that n-HA/PA66 prepared base on the n-HA sol has a better biocompatibility as compared with PA66.

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