Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (7): 1072-1077.doi: 10.3969/j.issn.2095-4344.0119
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Wang Gang, Xu Yi-ming, Ye Dong-mei, Fu Teng-fei, Zou Yu-zhen, Feng Xian-xuan, Bai Yue-hong
Online:
2018-03-08
Published:
2018-03-08
Contact:
Bai Yue-hong, M.D., Professor, Doctoral supervisor, Department of Rehabilitation, Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China
About author:
Wang Gang, M.D., Attending physician, Department of Rehabilitation, Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China
Supported by:
the Scientific Research Program of Science and Technology Commission of Shanghai City, No. 13231202600
CLC Number:
Wang Gang, Xu Yi-ming, Ye Dong-mei, Fu Teng-fei, Zou Yu-zhen, Feng Xian-xuan, Bai Yue-hong. Effects of different doses of microwave therapy on adjacent tissue of titanium alloy implants after fracture surgery[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(7): 1072-1077.
Quantitative analysis of animals None rabbits in the study dropped out, and all were involved in the result analysis. Temperature changes During microwave irradiation, we simultaneously measured the temperatures in deep muscle tissues. Temperature changes are shown in Figure 2. It was found that the temperature in the implanted group at 40 W was higher than that in the implanted group at 20 W (P < 0.000 1). In addition, the temperature in the implanted group at 40 W was higher than that in the control group at 40 W (P = 0.037 3). However, there was no significant difference between control and the implanted groups at 20 W (P = 0.547 9). The peak temperature of the deep muscles in the implanted group at 20 W was between 37.5 ℃ and 37.7 ℃. The peak temperature of the deep muscles in the implanted group at 40 W was between 40.3 ℃ and 40.6 ℃. Light microscopy Sections of the thigh muscle in each group were stained with hematoxylin and eosin after 30 days of microwave treatment. No abnormal morphological changes were found either in the control or implanted groups. Sections of sciatic nerve from each group were also stained with hematoxylin and eosin after the 30-day microwave treatment. The myelin sheaths of the sciatic nerves in the implanted group at 40 W were found to be swollen (Figure 3). Transmission electron microscopy Transmission electron microscope examinations of the skeletal muscle and sciatic nerves in each group were conducted after the 30-day microwave treatment. The muscles in the implanted group at 20 and 40 W showed mitochondrial swelling and vacuolization (mitochondrial damage). Myofilaments and myocommata were unclear. The myelin sheath of the sciatic nerves adjacent to the fractures at 20 W was normal. Part of the myelin sheath of myelinated nerve fibers showed lamellar separation, and ball-like changes in the pith were also observed. The mitochondria in the axons of the implanted group at 40 W were normal (Figure 4). No abnormal morphological changes in the sciatic nerve were discovered in the control group or the implanted group at 20 W. A KS400 image analysis system (Zeiss) was applied to calculate the percentage of the damaged mitochondrial area in each sample, to allow the quantitative analysis of mitochondrial damage in the muscle between two implanted groups, and found significant differences (Figure 5). The percentage of the damaged myelin sheaths of sciatic nerves adjacent to the fractures in the implanted group at 40 W was (31% ± 5%), while the myelin sheaths of sciatic nerves adjacent to the fractures in the implanted group at 20 W were normal. Thus, muscle and nerve injuries in the implanted group at 40 W were more serious than those at 20 W."
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