Imaging and histological changes of the healing process of experimental sheep femoral fracture using different fixation methods

doi:10.3969/j.issn.2095-4344.0118

Abstract

Abstract:

BACKGROUND: Ordinary bone plate fixation is prone to have "stress shielding" effect, and combined variable stress plate fixation can reduce the "stress shielding" effect of the plate, and promote the normal bone healing, reduce the complications caused by ordinary steel and shorten the fracture healing time.

OBJECTIVE: To explore the effects of different internal fixation methods on the imaging and histology in the healing process of experimental sheep femoral fracture.

METHODS: Thirty-two healthy adult goats were randomly divided into the experimental group and the control group with 16 cases in each group. The fractured animal model was constructed by femoral osteotomy. The experimental group and the control group were treated with combined variable stress plate and ordinary straight plate respectively for fixation. The X-ray, CT and histological methods were used to observe and compare callus 4, 8 and 12 weeks after fixation.

RESULTS AND CONCLUSION: (1) X-ray examination results showed that the fracture lines of the two groups were gradually from obscured to complete healing at 4 to 12 weeks after fixation, and the gap between the fracture ends was significantly reduced. When internal fixator was taken out, the callus around fracture end was little and hard and the bone plate was without callus coverage in the experimental group. There were a large number of callus covered in bone plate and large bone callus around the plate in the control group. (2) CT examination results showed that CT value and callus cross-sectional area value were gradually increased in two groups at 4 to 12 weeks after treatment. The values in the experimental group at 4, 8 and 12 weeks after treatment were significantly higher than those in the control group (P < 0.05), and the diameter of medullary cavity in the control group at 8 and 12 weeks after treatment was significantly higher than that in the experimental group (P < 0.05). The cortical bone thickness was significantly lower in the control group than that in the experimental group (P < 0.05). (3) Histological examination showed that compared with the control group, the fracture line was healed and the intramedullary fibrous callus was reduced and was sparse, and most of callus was absorbed and marrow cavity recanalization appeared in the experimental group. (4) The results show that combined variable stress plate fixation can provide a stable biomechanical environment for early healing of femoral fractures, reduce the stress shielding effect of steel plate and promote the callus formation, thereby helping fracture healing.

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

Key words: Tissue Engineering, Bony Callus, Bone Density

CLC Number:

R318

Feng Li-ping, Yang Wei-qiang, Ding Tong, Zhang Fu-mei, Jiang Zhen-gang, Wang Pei-shan. Imaging and histological changes of the healing process of experimental sheep femoral fracture using different fixation methods [J]. Chinese Journal of Tissue Engineering Research, 2018, 22(7): 1066-1071.

Figures/Tables 5

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