关键词: 慢性血栓栓塞性肺动脉高压, 模型, 实验动物, 心肺组织工程, 综述文献

Abstract:

OBJECTIVE: To summarize the research of construction of animal models of chronic thromboembolism pulmonary hypertension (CTEPH).
METHODS: The computer-based search was done in PubMed database from 2006 to 2009, with the key words of model of pulmonary thromboembolism”. Simultaneously, CNKI was retrieved for articles published from 2003 to 2009, with the key words of “pulmonary embolism, experiment”. Moreover, relevant book was retrieved by hand. Inclusion criteria: articles should address CTEPH experimental studies, including CTEPH epidemiology, different experimental animals and various preparation manners. Articles of the same circle published in recent years or in authorized journal. Exclusion criteria: repetitive study; Meta analysis.
RESULTS AND CONCLUSION: Experimental animals for CTEPH model preparation included dog, pig and rat. Many researchers tried to imitate CTEPH mechanism induced by multiple acute pulmonary embolism to establish animal models of CTEPH following deep venous thrombosis. To establish dog CTEPH models mainly contained repetitive autoblood clot injection and ceramics bead input, which could be used in study of thromboembolism pulmonary hypertension and imaging diagnostic technique, composing CTEPH pathophysiological characteristics and digital scissors-shadow tests, anatomy study, pulmonary artery and interventional therapy. In vitro injection embolus was mainly utilized to establish pig CTEPH models, followed by imaging technique to diagnose CTEPH. Repetitive autoblood clot injection and pulmonary artery ligation were employed to establish rat CTEPH models, which could be used for the study of CTEPH pathophysiology and diagnosis. At present, CTEPH study mainly used dog, pig and rat models, with advantages and disadvantages. In precise studies, suitable animals and preparation methods should be selected according to experimental objective and design to obtain an optimal experimental result.