中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2823-2827.doi: 10.3969/j.issn.1673-8225.2010.15.039

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

中国南方群体线粒体基因变异及载脂蛋白E基因与长寿的关联性

冯  洁1，2，刘  铭1，2，张建佚2，万  钢2，齐科研2，郑陈光3，吕泽平3，胡才有3，杨  泽2   

  1. 1北京协和医学院研究生院，北京市100730；2卫生部北京医院，卫生部老年医学研究所，北京市 100730；3广西壮族自治区江滨医院，广西壮族自治区南宁市 530021
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 通讯作者: 杨 泽，女，1955年生，博士，教授，卫生部老年医学重点实验室，卫生部北京医院，卫生部老年医学研究所，北京市 100730 yangze016@yahoo.com.cn
  • 作者简介:冯 洁☆，女，1979年生，山西省晋城市人，汉族，北京协和医学院在读博士，主要从事长寿的遗传学研究，北京市100730 fj79330@163.com
  • 基金资助:

    国家自然科学基金资助项目(30972709，30671110)。

MtDNA variants, apolipoprotein E gene and longevity in Southern Chinese population

Feng Jie 1,2, Liu Ming 1,2, Zhang Jian-yi2, Wan Gang2, Qi Ke-yan2, Zheng Chen-guang3, Lü Ze-ping3, Hu Cai-you3, Yang Ze2   

  1. 1 Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing   100730, China; 2 Key Laboratory of Geriatrics, National Institutes of Geriatrics, Beijing Hospital, Ministry of Health, Beijing   100730, China; 3 Jiangbin Hospital of Guangxi Zhuang Autonomous Region, Nanning   530012, Guangxi Zhuang Autonomous Region, China
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Yang Ze, Doctor, Professor, Key Laboratory of Geriatrics, National Institutes of Geriatrics, Beijing Hospital, Ministry of Health, Beijing 100730, China yangze016@ yahoo.com.cn
  • About author:Feng Jie☆, Studying for doctorate, Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China; Key Laboratory of Geriatrics, National Institutes of Geriatrics, Beijing Hospital, Ministry of Health, Beijing 100730, China fj79330@yahoo.com.cn
  • Supported by:

    the Grant from the Department of Science and Technology of Guangxi Zhuang Autonomous Region of China, No. Guikezi 0848016*

PDF

422

被引次数

2

摘要：

背景：有研究表明线粒体基因变异与长寿相关，但在中国南方群体中线粒体单倍组D及其亚组与长寿的相关性研究至今少有报道，并且线粒体单倍组D及其亚组与载脂蛋白E基因相互作用对长寿的影响未见报道。
目的：探讨在中国南方群体中线粒体单倍组D及其亚组与长寿的相关性，以及线粒体单倍组D及其亚组与载脂蛋白E基因的相互作用。
方法：于2007/2008在广西壮族自治区巴马县选取对象1 038人，包括长寿组367人(年龄> 90岁)和与长寿组无亲缘关系的当地健康对照组671人(年龄40~60岁)。采用多聚酶链反应-限制性片段长度多态性技术检测线粒体单倍组D及其亚组(D4和D4a)的分布情况，并通过分层分析调整性别和载脂蛋白E基因混杂因素的作用。
结果与结论：线粒体单倍组D及其亚组与长寿无明显相关性(P > 0.05)。按照性别进行分层分析后，在女性和男性群体中仍未发现线粒体单倍组D及其亚组与长寿的关联(P > 0.05)。然而，对载脂蛋白E基因分层分析后，在载脂蛋白E ε4携带者中长寿组和对照组线粒体单倍组D的分布频率差异有显著性意义(P < 0.05)。结果提示线粒体单倍组D可能是与载脂蛋白E基因的相互作用对长寿产生影响。

关键词: 长寿, 线粒体基因变异, 载脂蛋白E, 多聚酶链反应-限制性片段长度多态性, 基因多态性, 关联分析

Abstract:

BACKGROUND: MtDNA haplogroup D has been shown to influence longevity trait. However, the effects that mtDNA haplogroup D (and its subhaplogroups) have upon longevity in Southern Chinese population have rarely been reported, and the effect of reaction of mtDNA haplogroup D (and its subhaplogroup) and apolipoprotein E (APOE) gene on longevity remains poorly understood.
OBJECTIVE: To explore the association between mtDNA haplogroup D (and its subhaplogroup) and longevity in southern Chinese population, and the interaction of mtDNA haplogroup D (and its subhaplogroup) with APOE gene.
METHODS: A total of 1 038 people were selected from Bama, Guangxi between 2007 and 2008, including 367 long-lived individuals (aged > 90 years) and 671 local and unrelated younger controls (aged 40-60 years). Using polymerase chain reaction-restriction fragment length polymorphism, the distribution of mtDNA haplogroup D and its subhaplogroup (D4, D4a) was tested. The confounding effect of gender and APOE gene was detected by stratification analysis.
RESULTS AND CONCLUSION: There was no significant correlation between mtDNA haplogroup D (and its subhaplogroup) and longevity (P > 0.05). Stratification analysis by gender showed no significant difference for frequencies distribution of mtDNA haplogroup D and its subhaplogroup (D4, D4a) between long-lived individuals and controls in women and men (P > 0.05). However, the frequency distribution of mtDNA haplogroups D was different between long-lived individuals and controls with APOE ε4 carriers (P < 0.05) through stratification analysis by APOE gene. Results show that mDNA haplogroup D may affect longevity by interacting with APOE gene.

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