中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (10): 1593-1598.doi: 10.3969/j.issn.2095-4344.0721

• 生物材料综述 biomaterial review • 上一篇    下一篇

复合明胶甲基丙烯基水凝胶在骨组织工程中的应用及前景

刘肇兴1,吴  兴1,林欢欢1,刘  沙2,陈向军1,2 
  

  1. 1内蒙古医科大学研究生学院,内蒙古自治区呼和浩特市  010030;2解放军第253医院烧伤整形科,内蒙古自治区呼和浩特市  010030
  • 收稿日期:2017-11-28 出版日期:2018-04-08 发布日期:2018-04-08
  • 通讯作者: 陈向军,主任医师,硕士生导师,内蒙古医科大学研究生学院,内蒙古自治区呼和浩特市010030;解放军第253医院烧伤整形科,内蒙古自治区呼和浩特市 010030
  • 作者简介:刘肇兴,男,1987年生,内蒙古自治区鄂尔多斯市人,内蒙古医科大学在读硕士,医师,主要从事骨科及皮瓣外科的研究。

Application prospects of compound gelatin-methacryloyl hydrogel in bone tissue engineering

Liu Zhao-xing1, Wu Xing1, Lin Huan-huan1, Liu Sha2, Chen Xiang-jun1, 2
  

  1. 1School of Graduate, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 2Department of Burn and Plastic Surgery, the 253rd Hospital of Chinese PLA, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Received:2017-11-28 Online:2018-04-08 Published:2018-04-08
  • Contact: Chen Xiang-jun, Chief physician, Master’s supervisor, School of Graduate, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Liu Zhao-xing, Master candidate, Physician, School of Graduate, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China

摘要:

文章快速阅读:

 

文题释义:
复合明胶甲基丙烯基水凝胶:与天然细胞外基质具有诸多相似性,其结构上具有细胞黏附位点及基质金属蛋白酶水解位点,能够良好地支持细胞增殖与分布,己被广泛用于3D细胞培养和组织再生等方面的研究;存在的甲基丙烯酸基团使甲基丙烯酸酰基水凝胶具有光敏性,能够在紫外光照射下快速交联,理化性灵活可调,并可通过微制造方法,如微成形、微流体、自组装、光刻技术,生物打印等,合成出具有特定结构的可控框架。
复合明胶甲基丙烯基水凝胶的制备:是通过明胶和甲基丙烯酸在50 ℃下的PBS(pH=7.5)中直接反应合成的,该反应方式为甲基丙烯酸醛基与氨基酸残端的胺基和羟基进行反应,通过调节反应物中甲基丙烯酸的含量,实现不同程度的甲基丙烯酰基取代,继而产生具有不同物理性质的甲基丙烯酸酰基水凝胶。
 
摘要
背景:复合明胶甲基丙烯基水凝胶己被证实其在骨、软骨、心肌、血管等组织再生方面具有独特优势。
目的:归纳总结复合明胶甲基丙烯基水凝胶在骨组织工程中的研究新进展。
方法:检索PubMed数据库和CNKI数据库2010至2017年发表的文献,中文检索关键词为“明胶,甲基丙烯酸,水凝胶,羟基磷灰石,骨组织工程,骨再生,种子细胞,微环境”,英文检索关键词为“gelatin,methacrylamide,hydrogel,hydroxyapatite,bone tissue engineering,bone regeneration,seed cells,micro environment”。
结果与结论:可通过特定方式在复合明胶甲基丙烯基水凝胶中加入无机成分,以光刻、微流体、微成模及3D打印技术制备出仿生组织工程骨,使其在结构、力学和生物学特性上与天然骨组织相似,并具有成骨性。将复合明胶甲基丙烯基水凝胶与加载基因修饰的种子细胞置于一定条件下进行三维培养,经相关生物特性检测,发现植入支架中的种子细胞生长状态良好,并有与成骨再生性及血管再生性相关的基因表达。因此复合明胶甲基丙烯基水凝胶具有良好的体外成骨效果,是优良的骨组织工程材料。

关键词: 生物材料, 明胶, 甲基丙烯酸, 水凝胶, 羟基磷灰石, 骨组织工程, 骨再生, 种子细胞, 微环境

Abstract:

BACKGROUND: Compound gelatin-methacryloyl (GelMA) hydrogel has been shown to have unique advantages in bone, cartilage, myocardium, and vascular regeneration.
OBJECTIVE: To summarize the novel progress of compound GelMA hydrogel for bone tissue engineering.
METHODS: PubMed database and CNKI database from 2010 to 2017 were searched by using the keywords of “gelatin, methacrylamide, hydrogel, hydroxyapatite, bone tissue engineering, bone regeneration, seed cells, microenvironment” in Chinese and English, respectively.
RESULTS AND CONCLUSION: Inorganic components can be added in a specific way into the compound GelMA hydrogel to prepare tissue-engineered bone using photolithography, microfluidics, microfabrication and 3D printing techniques. The prepared tissue-engineered bone has similar structural, mechanical and biological properties to natural bone tissue, and importantly, it has osteogenic ability. Gene-modified seed cells that are co-cultured with the compound GelMA hydrogel in a 3D environment are found to grow well and express some genes related to bone regeneration and vascular regeneration. Therefore, the compound GelMA hydrogel has a good osteogenesis effect in vitro, which is an excellent material for bone tissue engineering.

Key words: Gelatin, Polymethacrylic Acids, Hydrogel, Bone Regeneration, Tissue Engineering

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