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    08 January 2015, Volume 19 Issue 2 Previous Issue    Next Issue
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    Serum transforming growth factor beta 1 contributes to bone healing in patients with bone fractures combined with spinal cord injuries
    Yang Sen, Wang Hai-long, Sheng Wei-bin, Xu Tao, Guo Hai-long
    2015, 19 (2):  165-169.  doi: 10.3969/j.issn.2095-4344.2015.02.001
    Abstract ( 271 )   PDF (659KB) ( 449 )   Save

    BACKGROUND: Studies have shown that transforming growth factor beta 1 plays an important role in the healing of fractures, but in spinal cord injury combined with fracture, transforming growth factor beta 1 has been never reported how to promote fracture healing.
    OBJECTIVE: To observe the changes of serum transforming growth factor beta 1 levels in patients with spinal cord injury combined with bone fractures and to discuss the mechanism of transforming growth factor beta 1 in fracture healing after spinal cord injury with bone fractures.
    METHODS: Totally 28 patients admitted at the Department of Spinal Surgery, the First Affiliated Hospital of Xinjiang Medical University from May 2012 to March 2014 were collected and divided into two groups according to whether they suffered from spinal cord injury: fracture+spinal cord injury group (n=12) and simple fracture group (n=16). Fasting peripheral blood samples were collected from patients in the two groups at 2, 7, 14, 28 days after injury, and ELISA method was used to detect the level of serum transforming growth factor beta 1.
    RESULTS AND CONCLUSION: There was no significant difference in the level of serum transforming growth factor beta 1 between the two groups at 2 days after injury (P > 0.05), but the levels of serum transforming growth factor beta 1 in the fracture+spinal cord injury group were lower than those in the simple fracture group at 7, 14, 28 days after injury (P < 0.05). In the fracture+spinal cord injury group, the level of serum transforming growth factor beta 1 had a rapid increase on the 7th day, and reached the peak on the 14th day, and then, this level had no significant decrease until the 28th day. In the simple fracture group, the level of serum transforming growth factor beta 1 began to increase on the 2nd day, reached the peak on the 7th day, and then decreased gradually. Remarkable changes of serum transforming growth factor beta 1 levels in patients with bone fracture combined with spinal cord injury may be associated with fracture healing in different periods.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Change rules and correlation between bone mass, bone turnover markers and estrogen levels in different periods of ovariectomized rats
    Shen Geng-yang, Ren Hui, Jiang Xiao-bing, Liang De, Yang Zhi-dong, Tang Jing-jing, Cui Jian-chao, Lin Shun-xin, Zhuang Hong, Zhang Shun-cong, Yao Zhen-song
    2015, 19 (2):  170-176.  doi: 10.3969/j.issn.2095-4344.2015.02.002
    Abstract ( 334 )   PDF (1005KB) ( 687 )   Save

    BACKGROUND: There are so many studies about ovariectomized rats at present, but the research on the change rules of bone mass, bone turnover markers, estrogen levels and their correlation in different periods of ovariectomized rats is rarely reported.
    OBJECTIVE: To analyze the change rules of bone mass, bone turnover markers, estrogen level and their correlation in different periods of ovariectomized rats.
    METHODS: Thirty-four 3-month-old female Sprague Dawley rats were randomly divided into three groups: baseline group, ovariectomized group and sham operated group. At the beginning of the experiment, the rats in the baseline group were sacrificed, then rats in the ovariectomized group and sham operated group were executed at 4, 8, 12 weeks postoperative respectively. The bone mineral density, bone mass content, area of different zones of the L1-3 lumbar vertebrae and femurs were detected by dual-energy X-ray absorption method, and meanwhile the serum levels of type I procollagen amino-terminal pro-peptide, I collagen carboxy-terminal peptide and estrogen were determined by ELISA. At last, we analyzed the correlation between body mass, bone mineral density in vitro, type I procollagen amino-terminal pro-peptide, I collagen carboxy-terminal peptide and estrogen levels and the age of ovariectomized rats.
    RESULTS AND CONCLUSION: (1) The bone mineral density and bone mass content of the lumbar vertebral and femurs in the ovariectomized group were significantly lower than those in the sham operated group and baseline group at the 4th week after operation (P < 0.05). The bone mineral density and bone mass content in the ovariectomized group were ameliorated obviously at the 8th and 12th weeks compared with those at the 4th week after operation (P < 0.05). The bone mass loss was highest in the L1 and intertrochanteric regions. (2) Serum levels of type I procollagen amino-terminal pro-peptide and I collagen carboxy-terminal peptide in the ovariectomized group were significantly higher than those in the baseline group and sham operated group at the 4th week after operation, but there was no difference at the 8th and 12th weeks. (3) The serum estrogen level in the ovariectomized group was prominently lower than that in the sham operated group and baseline group at the 8th and 12th weeks after operation (P < 0.01 at the 8th week, P < 0.05 at the 12th week). (4) The age was positively correlated with body mass and bone mineral density of the lumbar vertebrae and femurs in vitro, while the serum levels of type I procollagen amino-terminal pro-peptide and I collagen carboxy-terminal peptide were negatively correlated with the bone mineral density of the lumbar vertebrae and femurs in vitro (P < 0.01). These results suggested that the bone mass of the lumbar vertebrae and femurs in ovariectomized rats was decreased rapidly firstly, and then rose slowly with time; the bone mass in the L1 and intertrochanteric regions lost seriously; the bone turnover markers showed a significant increase at the beginning of ovariectomy and reduced gradually to normal condition, while the estrogen level was increased at the first month after ovariectomy and then decreased rapidly. In addition, the body mass, bone turnover markers and estrogen level were associated with the change of bone mass.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Correlation between the severity of knee osteoarthritis and levels of chemerin in serum and synovia
    Duan Guo-qing, Ren Chun-feng
    2015, 19 (2):  177-181.  doi: 10.3969/j.issn.2095-4344.2015.02.003
    Abstract ( 379 )   PDF (588KB) ( 594 )   Save

    BACKGROUND: Studies have found that after stimulation, chemerin can further amplify the inflammatory signal, resulting in the release of inflammatory factors and matrix metalloproteinases. It indicates that chemerin may serve as an important regulator of cartilage metabolism, and plays an important role in the pathogenesis of osteoarthritis.
    OBJECTIVE: To explore the correlation between level of chemerin in synovia and serum and severity of knee osteoarthritis.
    METHODS: Eighty patients with knee osteoarthritis were enrolled in the study. Knee osteoarthritis grading was evaluated according to the Kellgren-Lawrence classification. Chemerin levels in synovia and serum were examined with enzyme-linked immunosorbent assay.
    RESULTS AND CONCLUSION: The level of chemerin in synovia and serum in patients with knee osteoarthritis were positively correlated with the Kellgren-Lawrence classification (r=0.981, P < 0.001; r=0.901, P < 0.001). And there was a positive correlation between the level of chemerin in synovia and in serum (r=0.596, P < 0.01). Experimental findings suggest that the levels of chemerin in synovial fluid and serum that are positively correlated with the Kellgren-Lawrence classification can be used as a reference biomarker of severity of knee osteoarthritis.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Correlation between estrogen receptor gene polymorphism and bone mineral density in elderly women
    Wang Zeng-rong
    2015, 19 (2):  182-186.  doi: 10.3969/j.issn.2095-4344.2015.02.004
    Abstract ( 270 )   PDF (624KB) ( 550 )   Save

    BACKGROUND: Estrogen receptor α gene polymorphisms is certainly associated with osteoporosis, but there is still questionable for the study of risk genotypes.
    OBJECTIVE: To analyze the correlation between estrogen receptor gene polymorphism and bone mineral density in elderly women.
    METHODS: Totally 120 healthy elderly women were enrolled to extract the whole blood genomic DNA that was digested using Pvu II and Xba I, the distribution and frequency of genotypes were analyzed. Meanwhile, dual-energy X-ray absorptiometry measurements of bone mineral density with the femoral neck, greater trochanter and Ward’s triangle and were done.
    RESULTS AND CONCLUSION: Xba I digested genotypes included the XX in 6 cases, Xx in 78 cases, xx in 36 cases; Pvu II digested genotypes included the PP in 32 cases, Pp in 50 cases, pp in 38 cases. The age, menopause age and body mass index showed no differences among the different genotypes of elderly women  (P > 0.05). The bone mineral density values in the trochanter and Ward’s triangle at Pvu II digested PP genotype were significantly greater than those at Pp and pp genotypes (P < 0.05); while no significant differences were found in the bone mineral density values at different Xba I digested genotypes in elderly women (P > 0.05). These findings suggest that estrogen receptor gene polymorphism has certain correlation with the bone mineral density in elderly women, P alleles has certain effect to maintain the bone mineral density of the trochanter and Ward’s triangle in elderly women.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Adenovirus-mediated RNA interference against core binding factor alpha 1 inhibits the hypertrophic differentiation of chondrocytes
    Gao Bo, Xing Rong, Kong Qing-quan, Xiang Zhou, Yang Jing, Cai Jia-qin, Huang Yi-zhou,
    2015, 19 (2):  187-191.  doi: 10.3969/j.issn.2095-4344.2015.02.005
    Abstract ( 328 )   PDF (715KB) ( 798 )   Save

    BACKGROUND: Hypertrophic differentiation of chondrocytes is the sign of starting endochondral ossification, and it is also an essential step in endochondral ossification, which is a cascade reaction and difficult to be blocked once started. The end result is the formation of bone structure. RNA interference is a post-transcriptional gene silencing. Relevant studies have shown that the use of RNA interference to block the expression of core binding factor α1 (Cbfα1) can effectively inhibit the formation of heterotopic ossification.
    OBJECTIVE: To use RNA intereference technology to suppress Cbfα1 expression so as to achieve the purpose of blocking the hypertrophic diferentiation of chondrocytes.
    METHODs: We constructed an adenovirus containing siRNA against Cbfα1 (Ad-Cbfα1-siRNA). Retinoic acid and interleukin-1α were used to induce hypertrophic differetiation of chondrocytes, and then Ad-Cbfα1-siRNA was utilized to inhibit the hypertrophic differentiation of chondrocytes. Immunohistochemistry method was used to analyze the expression of Cbfα1.
    RESULTS AND CONCLUSION: After induction with retinoic acid and interleukin-1α, the chondrocytes in the negative control virus group appeared to have hypertrophy and the expression of Cbfα1 was positive. In the Ad-Cbα1-siRNA group, the expression of Cbfα1 was negative. These findings suggest that the inhibition of Cbfα1 by RNA interference can be a powerful way to prevent the hypertrophic differentiation of chondrocytes.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Diacerein interferes the expression of connective tissue growth factor in the articular cartilage cultured in vitro
    Wang Sheng, Liao Jun-lin, Liu Ri-guang
    2015, 19 (2):  192-195.  doi: 10.3969/j.issn.2095-4344.2015.02.006
    Abstract ( 283 )   PDF (606KB) ( 449 )   Save

    BACKGROUND: In osteoarthritis cartilage degeneration, connective tissue growth factors as the important effector molecules play an important role in chondrocyte proliferation and differentiation. Clinical application of diacerein for the treatment of osteoarthritis has achieved good effect, but the exact mechanism of the treatment is unclear. 
    OBJECTIVE: To observe the effect of different concentrations of diacerein on the expression of connective tissue growth factor in the articular cartilage induced with interleukin-1β in vitro.
    METHODS: Chondrocytes of Sprague Dawley rats were cultured in vitro, and then the chondrocytes were induced with recombinant human interleukin-1β to prepare the osteoarthritis model. Rats in the normal control group received no treatment; rats in the model group were treated with recombinant human interleukin-1β; rats in the experimental group were treated with different concentrations of diacerein+10 μg/L recombinant human interleukin-1β. The proliferation of chondrocytes was observed with MTT assay, and Western blot was used to detect the expression of connective tissue growth factor. The experiments above were repeated for three times.    
    RESULTS AND CONCLUSION: The MTT assay results showed that compared with the normal control group, the diacerein could promote the proliferation activity of chondrocytes, and more significant in the concentration of 10-5 mol/L (P < 0.01); the proliferation capacity of chondrocytes in the experimental group was decreased after induced with interleukin-1β (P < 0.05), but when compared with the normal control group, the diacerein with the concentrations of  10-4 mol/L and 10-5 mol/L could promote the MTT proliferation capacity of chondrocytes with or without interleukin-1β   (P < 0.05). Western blot results showed that interleukin-1β could reduce the expression of connective tissue growth factor (P < 0.01), and 10-5 mol/L diacerein could significantly promote the expression of connective tissue growth factor induced with interleukin-1β, and the effect was significantly higher than that in the model group (P < 0.01). Diacerein can promote expression of connective tissue growth factor induced with interleukin-1β, it may be one the mechanism of diacerein for promoting chondrocytes differentiation and proliferation and the treatment of osteoarthritis.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Bone bridge and bone plug fixation methods for meniscal allograft transplantation
    Peng Liang-quan, Zhu Wei-min, Wang Da-ping, Lu Wei, Feng Wen-zhe, Chen Kang
    2015, 19 (2):  196-200.  doi: 10.3969/j.issn.2095-4344.2015.02.007
    Abstract ( 314 )   PDF (679KB) ( 386 )   Save

    BACKGROUND: Studies have reported that the bone bridge and bone plug for meniscal allograft transplantation show better outcomes than simple soft tissue suture, but there are rare studies concerning the comparison of their fixation effects.
    OBJECTIVE: To compare the clinical effects of bone bridge and bone plug in meniscal allograft transplantation.
    METHODS: Eighteen patients undergoing arthroscopy-assisted meniscal allograft transplantation were enrolled, including nine cases of bone plug fixation and nine cases of bone bridge fixation. Symptoms, physical examination, visual analog scale, Lysholm and Tegner scores were used to evaluate the clinic outcome. Every 6 months post operation, the signal and morphological changes of allograft meniscus was observed with MRI for each patient.
    RESULTS AND CONCLUSION: All of the 18 patients were followed up for 12-36 months after transplantation. No complications, absence of knee pain and effusion, and disappearance of the discomfort within the meniscectomy compartments after prolonged activity during follow-up period were found in all patients. The patients were competent of daily life and sports activity. The visual analog scale, Lysholm and Tegner scores were significantly increased after transplantation. MRI showed that the vicariousness and livability of the allograft menisci appeared within 1 year post operation. The safety and feasibility of the arthroscopy-assisted meniscal allograft transplantation were approved. Knee pain, knee effusion and joint stability and function were improved after arthroscopic-assisted allograft meniscus transplantation, and there was no difference between the clinical curative effects of the two kinds of fixed methods.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Expression of hypoxia-inducible factor 2 alpha and vascular endothelial growth factor in chondrocytes of articular cartilages in human osteoarthritis
    Liu Feng, Peng Hao, Zhou Jian-lin, Fang Hong-song, Deng Shuang, Yang Xiao, Weng Jin-qing
    2015, 19 (2):  201-206.  doi: 10.3969/j.issn.2095-4344.2015.02.008
    Abstract ( 302 )   PDF (785KB) ( 477 )   Save

    BACKGROUND: Studies have found that vascular endothelial growth factor and hypoxia inducible factor are involved in the development process of osteoarthritis, but their correlation is rarely reported.
    OBJECTIVE: To observe the expression and correlation of hypoxia inducible factor-2α and vascular endothelial growth factor in chondrocytes of articular cartilages in human osteoarthritis.
    METHODS: Articular cartilage specimens were collected from 50 patients with knee osteoarthritis undergoing total knee joint replacement. According to the joint Kellgren-Lawrance (K-L) X-ray grouping classification standard, there were 18 cases of K-LIII level and 32 cases of K-LIV level. Besides, articular cartilage specimens from 10 patients undergoing amputation for legs tumor or traffic accident served as control group. Hematoxylin-eosin staining, Safranin O-Fast Green staining and Mankin scoring were performed to observe and evaluate the histological characteristics of articular cartilages of each group, immunohistochemical staining was conducted to detect the expression of hypoxia inducible factor-2α and vascular endothelial growth factor in chondrocytes of articular cartilages, and their correlations were analyzed.
    RESULTS AND CONCLUSION: The Mankin score of K-LIV group was significantly higher than those of K-LIII group and control group. Immunohistochemical staining revealed that the number of chondrocytes with positive expression of hypoxia inducible factor-2α or vascular endothelial growth factor in K-LIV group was significantly higher than that in K-LIII group and control group (P < 0.05). The expression of hypoxia inducible factor-2α and vascular endothelial growth factor increased in chondrocytes of articular cartilages of osteoarthritis patients, and to up-regulate the expression of vascular endothelial growth factor may be the regulatory mechanism of hypoxia inducible factor-2αin the pathogenesis of osteoarthritis.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Purification and culture methods of murine splenic B-lymphocytes
    Li Wen-juan, Zou Jia-qi, Han Xin-xin, Tian Zhi-hui, Liu Jie, Li Hai-dong
    2015, 19 (2):  207-212.  doi: 10.3969/j.issn.2095-4344.2015.02.009
    Abstract ( 428 )   PDF (666KB) ( 1759 )   Save

    BACKGROUND: B-lymphocytes are an important participant in the immunity system. Currently, magnetic beads and complement methods are mainly used to isolate and purify B-lymphocytes. However, these methods are costly or cause large cell damage and low purity, which need further improvement.
    OBJECTIVE: To explore the isolation and culture methods of B-lymphocytes from mouse spleen and to study suitable conditions for B-lymphocyte isolation and culture in vitro by using interleukin-4, lipopolysaccharide, CD3 monoclonal antibody or their combination.
    METHODS: B-lymphocytes from mouse spleen were isolated and randomly divided into seven groups, respectively treated with interleukin-4, CD3 monoclonal antibody, lipopolysaccharide, interleukin-4+CD3, interleukin-4+lipopolysaccharide, CD3+lipopolysaccharide, and no stimulation (control group). Flow cytometry was used to detect the changes in the number and proportion of T-lymphocytes, B lymphocytes, and their subpopulations under different culture conditions.
    RESULTS AND CONCLUSION: The number of lymphocytes peaked at 3-5 days after addition of interleukin-4. In the lipopolysaccharide group, the number of lymphocytes began to increase at 3 days, and then peaked at 5 days. T-lymphocytes disappeared after addition of CD3 monoclonal antibody, so relatively pure B-lymphocytes could be obtained after 2 days and the number of B-lymphocytes reached the peak at 3 days. The number of mature B-lymphocytes (B220+IgD+) increased significantly after addition of CD3 antibody. In all the conditions we tested, transitional B cell subset (B220+CD93+) disappeared completely after 24 hours of culture. Experimental results indicate that after addition of CD3 monoclonal antibody and interleukin-4, T-lymphocytes can be removed in mouse spleen cells cultured, but mature B-lymphocytes remain to survive and proliferate.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effects of diacetylmorphine on caspase-8 gene in apoptosis of cerebellar granule neurons
    Su Li-ping1, Liu Xiao-shan2, Wang Xue-mei, Chen Xiao, Zhang Li-ping, Pu Hong-wei, Wang Zhi-guo, Wang Hua, Li Kai-chao
    2015, 19 (2):  213-219.  doi: 10.3969/j.issn.2095-4344.2015.02.010
    Abstract ( 293 )   PDF (2372KB) ( 514 )   Save

    BACKGROUND: Caspase-8 plays an important role in starting the process of cell apoptosis, and diacetylmorphine can induce neuronal apoptosis. The relationship between the apoptosis of cerebellar granule neurons cells induced by diacetylmorphine and caspase-8 has not been reported.
    OBJECTIVE: To investigate the effect of caspase-8 on diacetylmorphine-induced neuronal apoptosis.
    METHODS: Cerebellar granule neurons from Sprague-Dawley rats aged 7 days were cultured in vitro. At 7 days, the cells were cultured with different dosage of diacetylmorphine (10, 40, 80, 100, 120 mg/L) and SP600125 for 24 hours, and were divided into control group, 80 mg/L diacetylmorphine group, diacetylmorphine+SP600125 group. Cell morphology was observed by Hoechst 33258 fluorescent staining, and cell apoptosis rate was measured by flow cytometry. Immunofluorescence staining, RT-PCR and western blot assay were used to detect caspase-8 mRNA and protein expression.
    RESULTS AND CONCLUSION: (1) After different dosage of diacetylmorphine was used to induce neuronal apoptosis, dark blue translucent apoptotic bodies were found in apoptotic cells, appearing with nucleus condensation, cohesion and fracture, and the apoptosis rate presented an increasing trend with increasing of drug dosage (P < 0.05). (2) Compared with the control group, caspase-8 mRNA and protein expression was remarkable under the intervention of 80 mg/L diacetylmorphine (P < 0.05); caspase-8 mRNA expression exhibited an increasing trend with increasing dosage of diacetylmorphine (P < 0.05), while caspase-8 mRNA and protein expression in the diacetylmorphine+SP600125 group was significantly lower than that in the 80 mg/L diacetylmorphine group (P < 0.05). These findings indicate that caspase-8 is involved in the process of diacetylmorphine-induced neuronal apoptosis, and meanwhile, it is also an important candidate of pro-apoptotic factors in the c-jun N-terminal kinase signaling pathway.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    MicroRNA-21 for regulation of TLR4 in Hela cells
    Zhao Jing, Yue Peng, Huang Jia-fang, Wang Yu-tao, Ma Ting, Chen Bao-rong
    2015, 19 (2):  220-224.  doi: 10.3969/j.issn.2095-4344.2015.02.011
    Abstract ( 365 )   PDF (643KB) ( 534 )   Save

    BACKGROUND: MicroRNAs (miRNA) through regulating specific target gene mRNA expression play important roles in different processes of diseases.
    OBJECTIVE: To study the interaction of miRNA-21 with its target gene TLR4 in Hela cells.
    METHODS: The candidate target gene of miRNA-21 was determined according to miRNA analysis databases. The constructed recombinant adenovirus vector carrying pri-miRNA-21 gene was used, which could package and amplify viruses to transfect Hela cells. Then, the expression of fluorescent proteins was detected. Forty-eight hours after transfection of miRNA-21 or control, extracted proteins were used for detection of TLR4 protein using western blot assay.
    RESULTS AND CONCLUSION: Recombinant adenoviruses pAd/pri-miRNA-21 and pAd/neg at 100 MOI could successfully infect Hela cells. Bioinformatic analysis suggested several possible binding sites between miRNA-21 and TLR4. The experimental results showed that miRNA-21 down-regulated TLR4 at protein levels, indicating that miRNA-21 can interfere with the expression of TLR4 target gene.

     


    中国组织工程研究
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    Dynamic conformational characteristics of the R102Q mutant of neuronal calcium sensor-1 protein
    Zhu Yu-zhen, Zhang Qing-wen
    2015, 19 (2):  225-230.  doi: 10.3969/j.issn.2095-4344.2015.02.012
    Abstract ( 291 )   PDF (631KB) ( 712 )   Save

    BACKGROUND: Neuronal calcium sensor-1 protein has a variety of different neuronal functions and has a high distribution in different areas of the brain. A single residue R102Q mutation in human neuronal calcium sensor-1 protein is demonstrated to be associated with autism disease. The experiment studies have reported that this R102Q mutant has essential conformation changes in local area of the neuronal calcium sensor-1.
    OBJECTIVE: To well understand the specific reasons of the R102Q mutation of the neuronal calcium sensor-1 to the conformational dynamic changes.
    METHODS: Six independent extensive all-atom molecule dynamic simulations during 0-450 ns were conducted.
    RESULTS AND CONCLUSION: We have found that (1) there is no obvious recombination during the simulations between wild type and mutant type, but R102Q mutant alters the helix and makes the structure of the protein more stable; (2) R102Q mutation alters the salt bridges, reduces the flexibility of L2, and makes L3 extend in hydrophobic crevice. These results reveal that the helix plays an important role in the structural stability, and salt bridge is the important reason for the dynamic changes of neuronal calcium sensor-1 protein. This study may provide a structural insight into the function of protein deficiency associated with R102Q mutant.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Association between the rs1007888 polymorphism of macrophage migration inhibitory factor gene and coronary heart disease in the Kazakhs of China
    Xu Rui, Yang Yi-ning, Ma Yi-tong, Li Xiao-mei, Zhao Qian, Chen Bang-dang, Liu Fen
    2015, 19 (2):  231-235.  doi: 10.3969/j.issn.2095-4344.2015.02.013
    Abstract ( 387 )   PDF (721KB) ( 460 )   Save

    BACKGROUND: Macrophage migration inhibitory factor (MIF) is a multi-potent cytokine that makes considerable contribution to the regulation of inflammatory response and immune response in the body. MIF rs1007888 is associated with various inflammatory diseases, but the correlation between rs1007888 and coronary heart disease in the Kazakhs of China has been rarely explored.
    OBJECTIVE: To investigate the relationship between rs1007888 gene polymorphisms in MIF gene and coronary heart disease in the Kazakhs from Xinjiang Uygur Autonomous Region, China.
    METHODS: A total of 230 Kazakh patients with coronary heart disease evidenced by coronary arteriography between December 2012 and July 2014 were recruited, and another 478 Kazak controls were free from coronary artery disease with normal angiograms. Real-time fluorescence quantitative PCR assay was used to detect the rs1007888 polymorphisms of MIF gene. Allele and genotype distributions of the rs1007888 polymorphism were 
    compared between patients and controls.
    RESULTS AND CONCLUSION: Distribution of genotypes in the two groups appeared to be in Hardy-Weinberg equilibrium (P > 0.05). The allele frequencies and genotypes of MIF-rs1007888 showed no significant difference between the two groups (P > 0.05). Therefore, the genetic variation of rs1007888 in MIF gene is not associated with coronary heart disease in the Kazakhs of China.

     


    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Interferon-gamma and interleukin-10 levels in serum and saliva are related to different types of oral lichen planus
    Zhu Jian-hua, Liu Na, Zhao Chang-rong, Liu Ji-guang
    2015, 19 (2):  236-240. 
    Abstract ( 198 )   PDF (332KB) ( 470 )   Save

    BACKGROUND: Many cytokines can be detected in saliva and serum, and have more clinical significance in the diagnosis, prognosis and treatment of oral mucosa disease.
    OBJECTIVE: To compare the interferon-γ and interleukin-10 levels in serum and saliva of patients with different types of oral lichen planus and to explore the feasibility of saliva samples as a substitute of blood samples to study the interferon-γ and interleukin-10 levels in serum and saliva.
    METHODS: Totally 45 patients with oral lichen planus admitted at the Department of Periodontology, the Stomatological Hospital of Jiamusi University from January to July 2014 were enrolled, including 15 cases of erosion type (erosion group), 15 cases of congestive erythema (congestive erythema group) and 15 cases of reticulate type (reticulate group). Another 15 healthy controls admitted for physical examination at the Department of Physical Examination, the Stomatological Hospital of Jiamusi University were enrolled as controls. ELISA method was used to detect the interferon-γ and interleukin-10 levels in serum and saliva in the four groups.
    RESULTS AND CONCLUSION: Compared with the control group, the interferon-γ levels in serum and saliva were lower in the other three groups (P < 0.01), while there were significant differences in the interferon-γ level among the patients with different types of oral lichen planus (P < 0.01). The interleukin-10 levels in serum and saliva were significantly higher in the erosion group and congestive erythema group than those in the control group (P < 0.01 or P < 0.05) and reticulate group P < 0.01 or P < 0.05). Experimental findings suggest that the levels of interferon-γ and interleukin-10 in serum and saliva are highly correlated in patients with different types of oral lichen planus, and saliva samples can be instead of blood samples to detect the levels of interferon-γ and interleukin-10 in patients with oral lichen planus.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Achilles tendon extension and tibialis transfer combined with tendon transposition for cerebral palsy-induced talipes equinovarus: ankle recovery and foot function evaluation
    Guo Jing-quan, Luo Yi, Gao Yu, Ren Shang-li, Zheng Zi-lei
    2015, 19 (2):  241-245.  doi: 10.3969/j.issn.2095-4344.2015.02.015
    Abstract ( 411 )   PDF (679KB) ( 499 )   Save

    BACKGROUND: It has a variety of treatments for equinovarus in spastic cerebral palsy, including physical therapy, orthotic therapy, systemic anti-spasticity drug therapy, local intramuscular injection of botulinum toxin type A and a variety of surgical operations. These treatments aim at different severity of deformities and different age in children patients, and each has its own merits.
    OBJECTIVE: To investigate the therapeutic effect of Achilles tendon “Z”-type extension, tibialis anterior and posterior transfer combined with tendon transposition to treat equinovarus in spastic cerebral palsy.
    METHODS: Twenty-two children with spastic cerebral palsy combined with equinovarus were collected from 2012 to 2014, and then these children were subject to Achilles tendon “Z”-type extension, tibialis anterior and posterior transfer combined with tendon transposition. Postoperative plaster external fixation was done for 6 weeks, and then rehabilitation training was given.
    RESULTS AND CONCLUSION: All the 22 patients were followed for an average of 1-2 years, and then were evaluated according to the degree of patient’s satisfaction and the degree of foot deformity. Satisfactory results were obtained from all the patients. Achilles tendon “Z”-type extension, tibialis anterior and posterior transfer combined with tendon transposition to treat spastic cerebral palsy combined with equinovarus is a good method, and it is characterized as good appearance of the ankle and favorable foot function.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Anterior cruciate ligament reconstruction with single beam of hamstring tendon: a 1-year follow-up of knee function and stability
    Chen Hui-hai, Wang Hong, Guo Zhe, Quan Liang-zhong, Tong Li, Wang Hui
    2015, 19 (2):  246-251.  doi: 10.3969/j.issn.2095-4344.2015.02.016
    Abstract ( 421 )   PDF (760KB) ( 637 )   Save

    BACKGROUND: Currently, there is still no clear conclusion on the pros and cons of arthroscopic anterior cruciate ligament reconstruction with or without stump preservation residues as well as the impact of reconstruction with stump preservation on proprioceptive sensation.
    OBJECTIVE: To compare the curative effect of arthroscopic anterior cruciate ligament reconstruction with or without stump preservation on the function and stability of the knee joint during a 1-year follow-up.
    METHODS: A retrospective analysis of 75 cases of arthroscopic anterior cruciate ligament reconstruction was done, including 37 cases of anterior cruciate ligament reconstruction without stump preservation and 38 cases of anterior cruciate ligament reconstruction with stump preservation. Autologous hamstring tendons were selected as implants. Evaluation of preoperative (6 months before operation) and postoperative (6 months and 1 year after operation) knee function and stability was implemented on the basis of Lysholm scores, IKDC Scores and KT2000. The comparative analysis was carried out as well on repeated passive angle for proprioception testing.
    RESULTS AND CONCLUSION: All of the patients were followed up for 12 to 20 months. Range of motion of the knee joint was basically restored in the two groups 6 months after operation. The results showed the Lysholm scores, IKDC scores and KT-2000 scores at 6 months and 1 year after operation were significantly improved in the two groups as compared with the preoperative data (P < 0.05), and there was no statistically difference between the two groups. The proprioception difference between the healthy and affected sides was lower in the patients with stump preservation than those without stump preservation at 6 months and 1 year after operation (P < 0.05). There were
    two cases in each group whose bone tunnel of the tibia was found deviating from the ideal position through postoperative X-ray examination, but cyclopia was unseen. Anterior cruciate ligament reconstruction with stump preservation is beneficial to the recovery of postoperative proprioception without increasing of postoperative complications.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Soft tissue stretch combined with physical factors improves trismus and mandibular function after third molar removal
    Fang Zhong-yi, Fan Shuai, Xu Li-li, Jiang Xin
    2015, 19 (2):  252-256.  doi: 10.3969/j.issn.2095-4344.2015.02.017
    Abstract ( 351 )   PDF (673KB) ( 704 )   Save

    BACKGROUND: The third molar extraction may lead to different degrees of soft tissue damage. Ultrasound and ultrashort waves belong to hyperthermia, which can increase tissue temperature and improve the therapeutic efficacy of soft tissue stretch.
    OBJECTIVE: To observe the effects of soft tissue stretch combined with ultrasound and ultrashort wave therapy for patients who were recently diagnosed with trismus and pain following third molar removal.
    METHODS: Sixteen patients with trismus and pain after third molar removal were randomly divided into two groups: patients in stretch group received soft tissue stretch with ultrasound and ultrashort wave therapy, while those in control group received only ultrasound and ultrashort wave therapy. Patients received the treatment five times per week, totally for 2 weeks.
    RESULTS AND CONCLUSION: It was found that after treatment pain was significantly reduced at the temporomandibular joint, and the range of maximum active mouth opening increased significantly. The improvement in mouth opening and temporomandibular joint function was better in the stretch group than the control group. These findings indicate that ultrasound and ultrashort wave therapy can improve symptoms of trismus and pain following third molar removal, and the combination of ultrasound and ultrashort wave therapy and soft tissue stretch therapy can achieve more effective results.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Non-living versus living-relative donor kidney transplantation: difference in perioperative blood transfusion
    Zhang Li, Feng Gui-wen, Shang Wen-jun, Wang Zhi-gang, Zhang Bo-wei, Kang Yi-qing, Yang He-cai
    2015, 19 (2):  257-261.  doi: 10.3969/j.issn.2095-4344.2015.02.018
    Abstract ( 254 )   PDF (635KB) ( 592 )   Save

    BACKGROUND: Uremia patients have different degree of anemia before kidney transplantation, preoperative, and perioperative anemia is harmful to intraoperative and postoperative recovery of the organism and renal function.
    OBJECTIVE: To observe the effects of perioperative anemia degree and total blood transfusion on non-living and living-relative donor kidney transplantation, and to summarize the perioperative drug treatment for anemia and perioperative principles of blood transfusion.
    METHODS: A retrospective study was done in 115 cases of non-living donor kidney transplantation (test group) and 92 cases of living-relative donor kidney transplantation (control group) from January 2012 to December 2013. Degree of anemia, total perioperative blood transfusion, electrolyte change within 12 hours of blood transfusion, and adverse events after blood transfusion were recorded and analyzed.
    RESULTS AND CONCLUSION: The incidence of anemia had no significant difference between the two groups (P > 0.05). Compared with the control group, the intraoperative and postoperative blood transfusion rate was higher in the test group (P < 0.05), and the preoperative red blood cell level, hemoglobin level, hematokrit, average hemoglobin level, average concentration of hemoglobin, and average amount of blood transfusions were significantly lower in the test group (P < 0.05). Fever (5.5%) was the main adverse event during the transfusion in the two groups, and there was no severe severe allergic reaction and electrolyte acid-base disturbance. These findings suggest that the perioperative degree of anemia is higher in patients undergoing non-living donor kidney transplantation and those undergoing living-relative donor kidney transplantation; preoperative drug treatment for anemia is crucial for correcting anemia status; intraoperative and/or postoperative blood transfusion treatment should be in strict accordance with the principles of perioperative blood transfusion.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Immunosuppressant therapy for pulmonary infection after kidney transplantation
    Yang Qi-shun, Zhang Lin, Zhang Zhi-qiang, Jiang Wei, Long Wei, Pei Xiang-ke
    2015, 19 (2):  262-266.  doi: 10.3969/j.issn.2095-4344.2015.02.019
    Abstract ( 297 )   PDF (571KB) ( 676 )   Save

    BACKGROUND: Pulmonary infection after kidney transplantation evolves rapidly. There is a high mortality rate in patients with server pulmonary infection. It has the important significance of early diagnosis and treatment of pulmonary infection, but some patients appear to have impaired kidney function because of the adjustment of immunosuppressants.
    OBJECTIVE: To explore the approaches to applying the immunosuppressants during the treatment of pulmonary infection after kidney transplantation.
    METHODS: The clinical data of 85 kidney transplantation patients who suffered from pulmonary infection were retrospectively analyzed. There were 43 cases in which the infection occurred within 1-6 months after kidney transplantation, 39 of which within 2-4 months; 7 cases of infection occurring within 6-12 months; 7 cases of infection within 12-24 months; 6 cases of infection within 24-36 months; 22 cases of infection occurring beyond 36 months. The immunosuppressant dose was adjusted based on a per-case basis. As a complement, the small-dose hormone was used for anti-inflammation. Etiological treatments for resisting infections were also conducted accordingly. Ventilators were utilized for patients with respiratory failures. The body temperature of patients was monitored and controlled. Appropriate nutrition support was also provided accordingly. There were 44 cases of decreasing or stopping the use of immunosuppressants during the early period of pulmonary infection; 19 cases of decreasing or stopping the use of immunosuppressants during the treatment of pulmonary infection; 5 cases of stopping the use of immunosuppressants during the period of severe pneumonia; 15 cases of gradually changing the dose of immunosuppressants during the early and progressive period of pneumonia; 2 cases of decreasing the use during the early period of pneumonia and stopping the use during the period of severe pneumonia. The duration of decreasing or stopping the use of immunosuppressants ranged from 3-51 days, with an average of 10.7 days.
    RESULTS AND CONCLUSION: Among the 85 patients, there were 81 cases cured and 4 cases of death. Among the four death cases, two cases died of acute respiratory failure and two cases died of multiple organ failure. Of the cured 81 cases, acute rejection occurred in 3 cases, while renal allograft dysfunction occurred in 6 cases. Decreasing or temporarily stopping the use of immunosuppressants during the treatment of pulmonary infection caused by the kidney transplantation increases the cure rate and decreases the mortality rate; while timely resuming the usage of immunosuppressants effectively protects the renal graft function, especially for patients with renal graft dysfunction.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Spinal cord injury and MicroRNA: in vitro prefabrication of tissue-engineered spinal cord and repair of spinal cord injury
    Li Mi, Yao Meng
    2015, 19 (2):  267-272.  doi: 10.3969/j.issn.2095-4344.2015.02.020
    Abstract ( 374 )   PDF (602KB) ( 455 )   Save

    BACKGROUND: In the whole world, spinal cord injuries caused by trauma lead to more than 180 000 people presenting with permanent impairment annually. A large number of experiments have confirmed in recent years, under physiological conditions, microRNA has specific expression and plays an important role in the nervous system.
    OBJECTIVE: To discuss the changes in microRNA expression induced by injuries as well as the pathophysiological significance in spinal cord injury, and to explore the development potential of microRNA in tissue-engineered and clinical repair of spinal cord injury.
    METHODS: A computer-based search of PubMed and Chinese Journal Database was performed for related articles published from January 2000 to December 2014 using the keywords of “SCI, microRNA, transcriptional control, clinical research progress” in English and Chinese. Finally, 38 articles were included for result analysis.
    RESULTS AND CONCLUSION: Mechanical injury initially triggers a series of complex secondary damages, including nervous, vascular and immune systems, which can influence the severity of spinal cord injury to a great extent. Secondary damage to the spinal cord is mainly attributed to the activation and deactivation of some specific genes associated with cellular and biochemical mechanisms, such as cysteine aspartate specific protease (caspase) gene family, apoptosis related protein Fas and its ligand Fasl system, P53 gene, apoptosis related gene Bcl-2 family. Recent studies have proved that the functional activation of microRNA expression is the key to spinal cord injury. With the development of biological information engineering, studies and controlling technologies associated with microRNA expression have been gradually dominated, some clinical application based on microRNA technology has entered the clinical trial stage. It is believed that with the continuous development of technology and decrease of cost, permanent dysfunction due to spinal cord injury can be regulated and repaired through the microRNA technology at gene level in the future.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Different viral vectors in gene-enhanced bone tissue engineering
    Xiao Zhen-tao, Guo Zhong-kai, Guo Li-xin
    2015, 19 (2):  272-276.  doi: 10.3969/j.issn.2095-4344.2015.02.021
    Abstract ( 314 )   PDF (594KB) ( 411 )   Save

    BACKGROUND: Various gene-delivery strategies have be used to transfer targeted genes into damaged bone tissues. As the most efficient gene vector, viral delivery systems have been used in bone tissue engineering research.
    OBJECTIVE: To thoroughly discuss the applications of different viral vectors in gene-enhanced bone tissue engineering.
    METHODS: A computer-based online search was performed in PubMed database for the related articles from January 2002 to January 2015. This review centered on viral vector transduction methods and their use in bone tissue engineering. Adenovirus, retrovirus, adeno-associated virus and chimeric virus were all discussed. Advantages and limitations of different vectors and their applicability toward bone tissue engineering were presented in this article. A total of 24 articles were included for review.
    RESULTS AND CONCLUSION: Current viral vectors for gene delivery in gene-enhanced bone tissue engineering are summarized, including recent work where combinatorial gene therapy is used to express groups of genes to stimulate bone regeneration. Future directions for this field are discussed, where viral vectors mediated gene expression systems will be combined with cells such as mesenchymal stem cells seeded in synthetic scaffolds to repair bone loss. Gene-enhanced bone tissue engineering has more advantages than traditional tissue engineering; viral vectors contribute to higher gene transfection efficiency than normal vectors. Long-term clinical observation is needed for the safety of viral vectors used in gene-enhanced tissue engineering in the body. Viruses are still the most efficient means by which exogenous genes can be introduced into seeds cells.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Curcumin prevents against osteoarthritis
    Zhang Rui, Liu Shi-qing
    2015, 19 (2):  277-282.  doi: 10.3969/j.issn.2095-4344.2015.02.022
    Abstract ( 595 )   PDF (938KB) ( 890 )   Save

    BACKGROUND: Curcumin is proved to possess anti-inflammatory, antioxidant and anti-apoptotic roles.
    OBJECTIVE: To summarize and discuss the mechanisms and effects of curcumin used for the treatment of osteoarthritis
    METHODS: PubMed, Embase, Elseveir databases were retrieved for relevant articles published from 1973 to 2014, with the key words of “osteoarthritis, curcumin, chondrocyte, articular cartilage” in English. After the quality of the included studies was evaluated, valid data were extracted and analyzed.
    RESULTS AND CONCLUSION: Curcumin prevents the occurrence and development osteoarthritis by inhibiting oxidative enzyme and scavenging free radicals. Curcumin increases production of collagen type 2 through suppressing the reduction of cartilage matrix by matrix metalloproteinases. Anti-inflammatory reaction of curcumin can be achieved by inhibiting cytosolic phospholipase A2, cyclooxygenase-2, and 5-lipoxygenase. Curcumin inhibits interleukin-1β-induced apoptosis of chondrocytes and mitochondrial swelling. Results from clinical trials suggest that curcumin or its derivatives can reduce joint pain and improve the function of knee joints in patients with osteoarthritis. High-concentration curcumin in vitro have been demonstrated toxic effects.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Brain-derived neurotrophic factor in the development and metabolism of bone and tooth: promoting or inhibiting proliferation and differentiation?
    Wu Xiao-ying, Yang Bin
    2015, 19 (2):  283-288.  doi: 10.3969/j.issn.2095-4344.2015.02.023
    Abstract ( 264 )   PDF (579KB) ( 562 )   Save

    BACKGROUND: Brain-derived neurotrophic factor has been detected in bone and tooth, and its role in development and metabolism of bone and tooth tissue as well as its clinical application has become a hot spot.
    OBJECTIVE: To summarize and analyze the effect and mechanism of brain-derived neurotrophic factor in development and metabolism of bone and tooth tissues.
    METHODS: Papers addressing the effect of brain-derived neurotrophic factor in bone and tooth tissue were retrieved by computer in Wanfang and PubMed databases with the key words of “brain-derived neurotrophic factor, TrkB, p75NTR, signaling, bone, tooth, osteoblasts, osteoclasts” in Chinese and English, respectively. A total of 53 papers were included for review.
    RESULTS AND CONCLUSION: Brain-derived neurotrophic factor can be detected in various tissues in vivo, and can regulate cell survival and apoptosis through binding its two receptors. Brain-derived neurotrophic factor in bone and tooth tissue can bind to target cells, induce or inhibit cell proliferation and differentiation, indicating that brain-derived neurotrophic factor is closely linked to bone and tooth tissue, and may play a role in growth and reconstruction of bone and tooth. Its mechanism of action is mainly through binding to TrkB receptor, to activate downstream pathways and affect differentiation and proliferation of mesenchymal stem cells, osteoblasts, chondrocytes, and periodontal ligament cells. Interaction between p75NTR receptor and TrkB receptor may be one of the factors affecting cell differentiation or proliferation.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Moxibustion: its outlook in the intestinal flora and mucosal immunity for regulation of irritable bowel syndrome
    Zhao Ji-meng, Huang Yan, Dou Chuan-zi, Wang Shuo-shuo, Wang Xiao-mei, Li Yin-he, Bao Chun-hui, Zhou Ci-li, Weng Zhi-jun, Lu Yuan, Wu Huan-gan
    2015, 19 (2):  289-293.  doi: 10.3969/j.issn.2095-4344.2015.02.024
    Abstract ( 494 )   PDF (669KB) ( 744 )   Save

    BACKGROUND: The interaction and mutual influence of intestinal flora in the host intestine with mucosal immune barrier play an important role in the homeostasis regulation within the intestine.
    OBJECTIVE: To explore the role of intestinal flora in the pathogenesis of irritable bowel syndrome and the mechanism of moxibustion in the treatment of irritable bowel syndrome from the aspects of intestinal flora imbalance and mucosal immune activation. 
    METHODS: With the key words of “irritable bowel syndrome, intestinal flora, intestinal immune, moxibustion” in Chinese and in English, respectively, a computer-based search was performed for articles published in CNKI, VIP and PubMed databases from October 2001 to October 2014. After the initial screening, the reserved articles were further detailed, summarized and concluded.
    RESULTS AND CONCLUSION: Totally 37 articles were collected, including 15 in Chinese and 22 in English. The analysis results showed that, the imbalance of intestinal flora and mucosal immune can play an important role in the pathogenesis of irritable bowel syndrome, and the former one can affect the body’s feeling of rectal distension threshold and visceral sensitivity. Moxibustion excels at improvement of the symptoms of irritable bowel syndrome, particularly abdominal pain with high visceral sensitivity, by regulating the body’s physiological balance from multi-link and multi-target aspects.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Epigenetic modification and the regulatory mechanism of moxibustion in inflammatory bowel disease
    Huang Yan1, 2, Dou Chuan-zi1, Huang Ren-jia2, Wu Lu-yi2, Wang Shuo-shuo3, Weng Zhi-jun1, Feng Hui4, Bao Chun-hui1, Liu Hui-rong1, Wu Huan-gan1
    2015, 19 (2):  294-299.  doi: 10.3969/j.issn.2095-4344.2015.02.025
    Abstract ( 341 )   PDF (629KB) ( 594 )   Save

    BACKGROUND: With the rapid development of science and technology, epigenetic modifications become hot, and the mechanism in inflammatory bowel disease has been partly revealed. Moxibustion can significantly improve the patients’ symptoms of Crohn’s disease and ulcerative colitis and reduce the side effects of drugs. But its underlying mechanisms have not been elucidated.
    OBJECTIVE: To explore the possible mechanisms of the epigenetic modification influencing inflammatory bowel diseases, to summarize the effector mechanisms of moxibustion in inflammatory bowel diseases, and to investigate the regulatory role of epigenetic modification in inflammatory bowel diseases by moxibustion.
    METHODS: A computer-based online research of PubMed, CNKI and VIP databases was performed with the key words of “epigenetic; inflammatory bowel disease; Crohn’s disease; ulcerative colitis; DNA methylation; histone modification; miRNA; moxibustion” in Chinese and English.
    RESULTS AND CONCLUSION: The correlation of epigenetic modification with inflammatory bowel disease occurrence and development is elaborated. The mechanisms in inflammatory bowel disease have been related from DNA methylation, histone modifications and miRNA targets. The mechanism of moxibustion on inflammatory bowel disease is primarily associated with immune regulation, and its anti-inflammatory mechanism may be affected by epigenetic regulation of inflammatory cytokines.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Construction strategy of human organ donation social mission system in China
    Cao Cui-ping, Huang Hai
    2015, 19 (2):  300-304.  doi: 10.3969/j.issn.2095-4344.2015.02.026
    Abstract ( 313 )   PDF (681KB) ( 728 )   Save

    BACKGROUND: By analyzing the current difficulties faced by human organ transplantation and social factors which lead to organ transplant donor shortage in China, people are found to be generally lack of knowledge about organ transplant and donation process.  
    OBJECTIVE: To make a tentative probe into the construction of organ donation social mission system on four aspects, namely the importance, ethical principles, current situation and countermeasures, and to provide scientific advice and information for organ donation publicity and education.
    METHODS: Articles about organ donation and organ transplantation were retrieved from CQVIP, CNKI, Wanfang, PubMed, Medline System from January 2005 to November 2014 with the key words of “organ donation, organ transplantation, shortage of donor, body donation, education system” in both Chinese and English. After secondary manual retrieval of organ donation coordinator, literature were included if they involved organ donation coordinator and organ donation and transplantation related policies and regulations. This paper put forward countermeasures for the construction of human organ donation social mission system in China based on the literature induction.
    RESULTS AND CONCLUSION: This paper describes the necessity of organ donation education system, systematically analyzes the existing problems for current organ donation education problems and ethical principles that should be followed, and explore the construction strategy of human organ donation social mission system in China. The way to relieve tension of organ transplant donor and expand donor sources is to win broad support and understanding of citizens, which can ensure the sustainable development of organ donation work. So, it is very important to build the human organ donation social mission system.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Efficacy and safety of alemtuzumab, daclizumab and antithymocyte globulin in kidney transplantation
    Wang Tao, Hao Wen-jing, Zong Huan-tao, Zhang Yong
    2015, 19 (2):  305-309.  doi: 10.3969/j.issn.2095-4344.2015.02.027
    Abstract ( 371 )   PDF (517KB) ( 665 )   Save

    BACKGROUND: Immunosuppressants fight against acute rejections by influencing humoral and cellular immune to suppress the immune function, and then improve patient/renal graft survival.
    OBJECTIVE:To evaluate the effectiveness and safety of alemtuzumab, daclizumab and antithymocyte globulin in inducing immunosuppression in kidney transplantation.
    METHODS:The randomized controlled trials of alemtuzumab or daclizumab versus ATG in kidney tranplantation published from 1966 to 2011 were enrolled by searching PubMed and EMBASE using Cochrane systematic review. We collected data and major outcomes. And a meta-analysis was conducted on homogeneous studies. 
    RESULTS AND CONCLUSION: A total of 9 randomized controlled trials (777 patients) about kidney transplantation were included. The meta-analysis results showed that the safety items including patient/renal graft survival and acute rejection at a follow-up of 24 months had no statistical differences among the three drugs (all  P > 0.05). But there was a significant difference between the infection rates of alemtuzumab and antithymocyte globulin at 36 months of follow-up (P < 0.05). The results indicate that alemtuzumab, daclizumab and antithymocyte globulin are equally effective in inducing immunosuppression at a follow-up of 24 months in kidney transplantation. However, compared to antithymocyte globulin, alemtuzumab has a lower rate of infection at a follow-up of 36 months.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    A Meta-analysis of carotid intima-media thickness and subclinical hypothyroidism
    Zhang Zhao-yun, Abulikemu Tuerdi
    2015, 19 (2):  310-315.  doi: 10.3969/j.issn.2095-4344.2015.02.028
    Abstract ( 346 )   PDF (810KB) ( 549 )   Save

    BACKGROUND: Recently, subclinical hypothyroidism has been associated with increased carotid intima-media thickness, but it is controversial.
    OBJECTIVE: To assess whether carotid intima-media thickness in patients with subclinical hypothyroidism differs from that in euthyroid subjects.
    METHODS: We searched published studies concerning the carotid intima-media thickness of patients with subclinical hypothyroidism in comparison with euthyroid subjects. Then, we evaluated each potential study for eligibility, assessed the methodological quality, and extracted the data for a Meta-analysis.
    RESULTS: Eight observational studies with 3 602 cases met the eligibility criteria. In patients with subclinical hypothyroidism, the pooled estimate of the weighted mean difference (WMD) of increased carotid intima-media thickness was 0.056 [95% CI (0.020, 0.092)]. Sensitivity analysis using a pooled result of the seven higher-quality studies demonstrated higher carotid intima-media thickness level in patients with subclinical hypothyroidism than in euthyroid subjects [WMD=0.064, 95% CI (0.024, 0.105)]. In a subgroup analysis, subclinical hypothyroidism was even more significantly associated with the carotid intima-media thickness in patients with a mean thyrotropin level > 10.0 mU/L [WMD=0.082, 95% CI (0.049, 0.116)]. Subclinical hypothyroidism was also associated with a significant increase in systolic blood pressure, triglyceride levels, total cholesterol levels, low-density lipoprotein levels and with a decrease in fasting plasma glucose. This meta-analysis indicates that subclinical hypothyroidism 
    is associated with an increased carotid carotid intima-media thickness, which may be due to elevated thyrotropin, dyslipidemia and hypertension. Despite the obvious individual differences, a prospective large-sample study is necessary to further assess the conclusions of this observation.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    A Meta-analysis of tubular stomach versus whole stomach for digestive tract reconstruction
    Liu Lan-bo, Qi Hai, Zheng Shi-yi
    2015, 19 (2):  316-321.  doi: 10.3969/j.issn.2095-4344.2015.02.029
    Abstract ( 410 )   PDF (688KB) ( 726 )   Save

    BACKGROUND: Over the past 10 years, scholars have proposed the tubular stomach as an alternative to the whole stomach for digestive tract reconstruction; however, its occurrence rate of postoperative complications has been controversial.
    OBJECTIVE: To evaluate the clinical efficacy of tubular stomach versus whole stomach for digestive tract reconstruction in the resection of esophageal carcinoma.
    METHODS: The randomized controlled trials about tubular stomach for digestive tract reconstruction in the resection of esophageal carcinoma were searched from PubMed, OVID, CNKI, EBSCO, Science online, Wangfang, Super Star Digital Library, CMB, Baidu and Google search engines. Two searchers screened studies based on the included criteria strictly. Literature quality and bias risk were assessed according to the criteria of Cochrane Collaboration, GRADEprofiler3.6.1 software was used for evaluation of the quality grade, and Revman5.3 for data management and statistical analysis.
    RESULTS AND CONCLUSION: Totally 12 randomized controlled trials including 4 137 patients were enrolled. Compared with the whole stomach group, in the tubular stomach group, the incidences of reflux esophagitis and thoracic stomach syndrome were significantly lower, but there was no difference in the incidences of anastomotic leakage and anastomotic stenosis between the two groups. These findings indicate that the tubular stomach as a substitute of the whole stomach for digestive tract reconstruction in the resection of esophageal carcinoma is a safe and effective. However, the literatures included are only in English and Chinese, and there is publication bias and small sample size. Therefore, the large-sample high-quality clinical randomized controlled trials are still needed for further confirmation.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Clinical efficacy of central pancreatectomy and distal pancreatectomy: a Meta-analysis of the reserved endocrine and exocrine functions of the pancreas
    Cao Xing-hua, He Tie-ying, Lin Hai, Han Wei, Chen Qi-long
    2015, 19 (2):  322-328.  doi: 10.3969/j.issn.2095-4344.2015.02.030
    Abstract ( 380 )   PDF (650KB) ( 519 )   Save

    BACKGROUND: Central pancreatectomy is a surgical treatment for tumors at the neck or the middle part of the pancreas, which can reserve more normal pancreas, not cut adjacent organs, and reduce the incidence of postoperative internal and external pancreatic secretion deficiency with respect to the expanded proximal and distal pancreatectomy. 
    OBJECTIVE: To systematically evaluate the clinical efficacy of the central pancreatectomy and distal pancreatectomy.
    METHODS: A computer-based search of Chinese and English databases was performed, and then 15 controlled clinical trials were included and systematically evaluated using RevMa5.2 software.
    RESULTS AND CONCLUSION: Totally 1 079 cases were included in this study, which consisted of 436 central pancreatectomy cases and 643 distal pancreatectomy cases. Meta-analysis showed that compared with the distal pancreatectomy group, the incidences of postoperative pancreatic fistula and complications were significantly higher, the risk of postoperative endocrine and exocrine insufficiency were significantly lower, while the surgical time (SMD: 59.23, 95% CI: 22.41-96.05, P < 0.01) and hospital stays (SMD: 7.01, 95% CI: 1.94-12.09, P < 0.01) were longer in the central pancreatectomy group. These findings indicate that although the central pancreatectomy has a high postoperative complication incidence, it can be accepted clinically, which may be a reasonable operation method to preserve pancreatic exocrine and endocrine function.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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