Change rules and correlation between bone mass, bone turnover markers and estrogen levels in different periods of ovariectomized rats

doi:10.3969/j.issn.2095-4344.2015.02.002

Abstract

Abstract:

BACKGROUND: There are so many studies about ovariectomized rats at present, but the research on the change rules of bone mass, bone turnover markers, estrogen levels and their correlation in different periods of ovariectomized rats is rarely reported.
OBJECTIVE: To analyze the change rules of bone mass, bone turnover markers, estrogen level and their correlation in different periods of ovariectomized rats.
METHODS: Thirty-four 3-month-old female Sprague Dawley rats were randomly divided into three groups: baseline group, ovariectomized group and sham operated group. At the beginning of the experiment, the rats in the baseline group were sacrificed, then rats in the ovariectomized group and sham operated group were executed at 4, 8, 12 weeks postoperative respectively. The bone mineral density, bone mass content, area of different zones of the L1-3 lumbar vertebrae and femurs were detected by dual-energy X-ray absorption method, and meanwhile the serum levels of type I procollagen amino-terminal pro-peptide, I collagen carboxy-terminal peptide and estrogen were determined by ELISA. At last, we analyzed the correlation between body mass, bone mineral density in vitro, type I procollagen amino-terminal pro-peptide, I collagen carboxy-terminal peptide and estrogen levels and the age of ovariectomized rats.
RESULTS AND CONCLUSION: (1) The bone mineral density and bone mass content of the lumbar vertebral and femurs in the ovariectomized group were significantly lower than those in the sham operated group and baseline group at the 4th week after operation (P < 0.05). The bone mineral density and bone mass content in the ovariectomized group were ameliorated obviously at the 8th and 12th weeks compared with those at the 4th week after operation (P < 0.05). The bone mass loss was highest in the L1 and intertrochanteric regions. (2) Serum levels of type I procollagen amino-terminal pro-peptide and I collagen carboxy-terminal peptide in the ovariectomized group were significantly higher than those in the baseline group and sham operated group at the 4th week after operation, but there was no difference at the 8th and 12th weeks. (3) The serum estrogen level in the ovariectomized group was prominently lower than that in the sham operated group and baseline group at the 8th and 12th weeks after operation (P < 0.01 at the 8th week, P < 0.05 at the 12th week). (4) The age was positively correlated with body mass and bone mineral density of the lumbar vertebrae and femurs in vitro, while the serum levels of type I procollagen amino-terminal pro-peptide and I collagen carboxy-terminal peptide were negatively correlated with the bone mineral density of the lumbar vertebrae and femurs in vitro (P < 0.01). These results suggested that the bone mass of the lumbar vertebrae and femurs in ovariectomized rats was decreased rapidly firstly, and then rose slowly with time; the bone mass in the L1 and intertrochanteric regions lost seriously; the bone turnover markers showed a significant increase at the beginning of ovariectomy and reduced gradually to normal condition, while the estrogen level was increased at the first month after ovariectomy and then decreased rapidly. In addition, the body mass, bone turnover markers and estrogen level were associated with the change of bone mass.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Osteoporosis, Postmenopausal, Estrogens, Ovariectomy

CLC Number:

R318

Shen Geng-yang, Ren Hui, Jiang Xiao-bing, Liang De, Yang Zhi-dong, Tang Jing-jing, Cui Jian-chao, Lin Shun-xin, Zhuang Hong, Zhang Shun-cong, Yao Zhen-song. Change rules and correlation between bone mass, bone turnover markers and estrogen levels in different periods of ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(2): 170-176.

Figures/Tables 5

0周分别增长了16.97%，27.52%，46.33%。大鼠去卵巢后体质量增加更为迅速，在术后4，8，12周时较0周分别增长了44.50%，65.60%，83.73%，从术后4周开始体质量显著重于同时期的假手术组大鼠(P < 0.01)。术后第4，8，12周去卵巢组子宫质量较基线组(P < 0.05)、假手术组显著降低(P < 0.01)；术后第4，8，12周去卵巢组肾上腺质量与基线组、假手术组差异均无显著性意义(P > 0.05)。 2.3 术后不同时期大鼠离体骨DXA扫描结果 2.3.1 不同时期离体腰椎DXA扫描结果见图2。去卵巢后4周去卵巢组离体腰椎各感兴趣区域骨矿含量、骨密度和骨面积均较基线组、假手术组明显降低(P < 0.05)，与基线组比较，腰椎各感兴趣区域骨量丢失幅度依次为15.77%(L1)、14.11%(L2)、13.45%(L3)。去卵巢后第8，12周时L1、L2、L3及L1-3的骨密度、骨矿含量较假手术组降低(P < 0.05)，但骨面积与假手术组比较差异无显著性意义，同时相对去卵巢后第4周而言均显著改善(P < 0.05)。大鼠在去卵巢后腰椎骨量呈先快速降低、再缓慢回升的趋势，但整个观察期内都比假手术组要低。 2.3.2 不同时期离体股骨DXA扫描结果见图3。去卵巢后4周去卵巢组大鼠离体股骨各感兴趣区域骨矿含量、骨密度、骨面积较基线组、假手术组均显著降低(P < 0.05)，与基线组比较，股骨各感兴趣区域骨量丢失幅度依次为12.44%(R2转子区)、11.60%(R1头颈区)、3.24%(R3股骨干区)。去卵巢后第8，12周时R1、R2、R3、R4的骨矿含量、骨密度较假手术组降低(P < 0.05)，但骨面积与假手术组比较差异无显著性意义，同时相对去卵巢后第4周而言均显著改善(P < 0.05)。大鼠在去卵巢后股骨骨量变化呈先快速降低，再缓慢回升的趋势，但整个观察期内都比假手术组要低。"

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