Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (25): 4020-4025.doi: 10.3969/j.issn.2095-4344.2017.25.014
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Kang Cong1, Meng Xian-yong2, Yang Xin-ming2, Cheng Yao-yu1, Zhang Zhen-liang1
Revised:
2017-03-20
Online:
2017-09-08
Published:
2017-10-09
Contact:
Meng Xian-yong, Associate chief physician, Department of Orthopedics, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China
About author:
Kang Cong, Studying for master’s degree, Hebei North University, Zhangjiakou 075000, Hebei Province, China
Supported by:
the Medical Research Project of Hebei Provincial Health Department in 2011, No. 20110176
CLC Number:
Kang Cong, Meng Xian-yong, Yang Xin-ming, Cheng Yao-yu, Zhang Zhen-liang. Combined use of low-dose 17-beta estradiol and bone marrow mesenchymal stem cell transplantation for spinal cord repair[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(25): 4020-4025.
2.4 TUNEL染色结果 TUNEL染色显示,凋亡阳性细胞的胞核内存在棕黄色颗粒。假手术组组切片组织中仅见少量散在的TUNEL阳性细胞,神经元细胞结构完整,核膜清晰;其余各组造模后不同时间点均可检测到较多TUNEL阳性细胞,呈现典型的凋亡特征性,凋亡的细胞结构模糊,细胞核固缩,胞浆浓染。 模型组、雌激素组、干细胞组、联合组造模后6 h阳性细胞较假手术组增多,主要位于灰质;12 h时,损伤部位阳性细胞明显增多,达到损伤高峰,白质中也开始出现阳性细胞;24 h时,白质中阳性细胞数量减少;72 h时,阳性细胞较前减少且阳性细胞主要位于白质脱髓鞘区域,见图2。 造模后72 h,假手术组、模型组、雌激素组、干细胞组、联合组的TUNEL阳性细胞率分别为(2.74±0.51)%、(36.42±2.46)%、(15.56±2.85)%、(18.92±2.73)%、(6.97± 2.08)%;模型组TUNEL阳性细胞率高于假手术组(P < 0.05),雌激素组、干细胞组、联合组TUNEL阳性细胞率低于模型组(P < 0.05),联合组低于雌激素组、干细胞组(P < 0.05)。"
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