Diacerein interferes the expression of connective tissue growth factor in the articular cartilage cultured in vitro

doi:10.3969/j.issn.2095-4344.2015.02.006

Abstract

Abstract:

BACKGROUND: In osteoarthritis cartilage degeneration, connective tissue growth factors as the important effector molecules play an important role in chondrocyte proliferation and differentiation. Clinical application of diacerein for the treatment of osteoarthritis has achieved good effect, but the exact mechanism of the treatment is unclear.
OBJECTIVE: To observe the effect of different concentrations of diacerein on the expression of connective tissue growth factor in the articular cartilage induced with interleukin-1β in vitro.
METHODS: Chondrocytes of Sprague Dawley rats were cultured in vitro, and then the chondrocytes were induced with recombinant human interleukin-1β to prepare the osteoarthritis model. Rats in the normal control group received no treatment; rats in the model group were treated with recombinant human interleukin-1β; rats in the experimental group were treated with different concentrations of diacerein+10 μg/L recombinant human interleukin-1β. The proliferation of chondrocytes was observed with MTT assay, and Western blot was used to detect the expression of connective tissue growth factor. The experiments above were repeated for three times.
RESULTS AND CONCLUSION: The MTT assay results showed that compared with the normal control group, the diacerein could promote the proliferation activity of chondrocytes, and more significant in the concentration of 10-5 mol/L (P < 0.01); the proliferation capacity of chondrocytes in the experimental group was decreased after induced with interleukin-1β (P < 0.05), but when compared with the normal control group, the diacerein with the concentrations of 10-4 mol/L and 10-5 mol/L could promote the MTT proliferation capacity of chondrocytes with or without interleukin-1β (P < 0.05). Western blot results showed that interleukin-1β could reduce the expression of connective tissue growth factor (P < 0.01), and 10-5 mol/L diacerein could significantly promote the expression of connective tissue growth factor induced with interleukin-1β, and the effect was significantly higher than that in the model group (P < 0.01). Diacerein can promote expression of connective tissue growth factor induced with interleukin-1β, it may be one the mechanism of diacerein for promoting chondrocytes differentiation and proliferation and the treatment of osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Osteoarthritis, Chondrocytes, Connective Tissue Growth Factor, Interleukin-1

CLC Number:

R318

Wang Sheng, Liao Jun-lin, Liu Ri-guang. Diacerein interferes the expression of connective tissue growth factor in the articular cartilage cultured in vitro[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(2): 192-195.

Figures/Tables 2

References

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