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    11 June 2014, Volume 18 Issue 24 Previous Issue    Next Issue
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    Micro CT study on trabecular microstructure of tibial plateau in ovariectomized goats
    Wang De-zhi, Chen Shi-chang, Liang Zheng-yang, Wang You
    2014, 18 (24):  3773-3778.  doi: 10.3969/j.issn.2095-4344.2014.24.001
    Abstract ( 269 )   PDF (841KB) ( 422 )   Save

    BACKGROUND: Trabecular microstructure of tibial plateau changes significantly under the progress of osteoporosis. Micro CT is a comprehensive, three-dimensional, noninvasive technology, which provides new method of measuring bone microstructure, evaluating bone quality and predicting bone strength. In recent years, this technique has been widely used in the field of osteoporosis study.
    OBJECTIVE: To investigate the trabecular microstructure of tibial plateau in ovariectomized goats by Micro CT.
    METHODS: Twelve female goats at 2.5 years old were randomly divided into ovariectomy (OVX) and sham groups, and each group included six goats. Goats in OVX group underwent ovarian surgery, while sham-operated goats removed equal amount of abdominal fat tissue. Half of goats in both OVX and sham groups were executed at 2 and 4 years after surgery, respectively. The left tibial plateau was harvested and a high resolution Micro CT was used to identify the trabecular microstructure parameters of metaphysis and epiphysis.
    RESULTS AND CONCLUSION: Compared with the sham-operated goats at 2 and 4 years post-surgery, bone volume fraction, trabecular number and trabecular thickness were decreased in the OVX group (P < 0.05), while trabecular separation was increased (P < 0.05), which all appeared a time-dependent manner. As exceptions, for the comparison of trabecular thickness in epiphysis between OVX group and sham group at 4 years post-surgery  (P > 0.05), and for the comparison of both trabecular thickness and trabecular separation in epiphysis at 2 years post-surgery (P > 0.05), there was no significant difference. At 2 and 4 years after surgery, microstructure parameters in metaphysis changed more obviously than them in epiphysis. The trabecular microstructure showed the differences between metaphysis and epiphysis of tibial plateau. The change of trabecular microstructure appears to be site-specific in the progress of osteoporosis in tibial plateau, and the microstructure deterioration of trabecular bone in the metaphysis of tibial plateau happens severer than that in the epiphysis.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Tibiofemoral joint contact area and biomechanical changes after partial meniscectomy
    Zhang Yuan-min, Wang Guo-dong, Zhang Yu-ge, Zhao Xiao-wei, Wang Lei, Li Bing-liang, Kong Ying
    2014, 18 (24):  3779-3784.  doi: 10.3969/j.issn.2095-4344.2014.24.002
    Abstract ( 487 )   PDF (641KB) ( 516 )   Save

    BACKGROUND: Biomechanical experiments contribute to elucidate the progressive cartilage lesions following meniscectomy. The causes of articular cartilage degeneration are very complex, including biology, mechanics and structural pathway.
    OBJECTIVE: To observe the changes of tibiofemoral joint contact area, pressure and strain after serial meniscectomies of the caudal pole of the medial meniscus.
    METHODS: Unpaired pelvic limbs were harvested from eight hybrid adult dogs. All specimens underwent sequential meniscectomy, and the range of resected meniscus in each specimen was calculated as the percentage: a 30% radial width partial meniscectomy, a 75% radial width partial meniscectomy, and a segmental caudal pole hemi-meniscectomy. Biomechanical tests were performed using MTS 858. The difference between groups was compared with statistical analysis using SPSS 18.0 software.
    RESULTS AND CONCLUSION: More tissues removed after meniscectomy, more obviously the medial and lateral tibiofemoral joint contact area declined. After a 70% radial width meniscectomy and total meniscectomy, the medial contact area showed no significant difference (P > 0.05); after the total meniscectomy, the lateral contact area was significantly reduced. After a 70% radial width meniscectomy and total meniscectomy, the medial contact pressure was significantly increased, showing significant differences compared with control group (P < 0.05); after the total meniscectomy, the lateral contact pressure was significantly decreased (P < 0.058). A meniscectomy resulted in a decrease in medial meniscal strain, but there was no significant difference between groups. The range of meniscectomy determines the biomechanical function post-operation; as for the meniscus tear, we suggest meniscal suture, to reduce the range of meniscectomy, avoid or delay the degeneration of knee joint.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Differential expression of matrix structural proteins in cerebrovascular malformations
    Yang Zhen-cun, Wang Zeng-liang, Dang Mu-ren, Lin Ling-chao, Serick Duysenb
    2014, 18 (24):  3785-3790.  doi: 10.3969/j.issn.2095-4344.2014.24.003
    Abstract ( 297 )   PDF (2598KB) ( 428 )   Save

    BACKGROUND: Studies have shown that the wall structure matrix protein expression has some differences in brain vascular malformations, but the difference between the structure matrix proteins of vascular malformations and the correlation between structural protein difference and vascular malformations are rarely reported.
    OBJECTIVE: To evaluate the differential expression of matrix structural proteins in vascular malformations of the brain.
    METHODS: Fifty surgical specimens of cerebral vascular malformation and 34 normal specimens of superficial temporal artery were subject to hematoxylin-eosin staining. Single/polyclonal antibodies, produced by Santa Cruz, USA against four structural proteins matrix (IV collagen, a-smooth muscle protein, laminin, fibronectin) were applied for immunohistochemical staining.
    RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that, cerebral vascular malformations showed significant disorder and constitute heteromorphism compared with normal vascular wall structure; in vascular malformations, there were obvious differences in cerebral arteriovenous malformations and cavernous hemangioma structure. Immunohistochemical staining showed that, in the normal temporal artery and vascular 
    malformations, IV collagen and smooth muscle actin-a were both positively expressed, and the positive rate had no difference, but the differences were significant in the structural arrangement. Laminin and fibronectin expression rate showed statistically significant differences between normal temporal artery and vascular malformations (P < 0.05), normal vascular wall expressed more laminin, while vascular malformations expressed more fibronectin. Laminin and fibronectin expression rate also had significant differences between cerebral arteriovenous malformation and cavernous hemangioma (P < 0.05), cerebral arteriovenous malformation expressed more laminin, while cavernous hemangioma expressed more fibronectin. The results confirmed that, compared with normal cerebral vessels, cerebral vascular malformations’ wall structure appears obvious heteromorphism, IV collagen and smooth muscle actin-a have apparent structural disorder, with low expression of laminin and high expression fibronectin, this difference may be the cause of abnormal cerebral wall structure.



    中国组织工程研究
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    Fixation effects of four different complex fixatives on mouse retina
    Pei Xi, Wu Qiu-ping, Ke Xiao-yun, Peng Yan-yang, Chen Xiao-hong
    2014, 18 (24):  3791-3796.  doi: 10.3969/j.issn.2095-4344.2014.24.004
    Abstract ( 521 )   PDF (2364KB) ( 592 )   Save

    BACKGROUND: An optimal fixative, which can avoid retinal detachment, has been the subject of intensive experiments and clinical pathological studies.
    OBJECTIVE: To compare the fixation effects of four different complex fixatives on mouse retina.
    METHODS: Forty Kunming mice were sacrificed, and the eyeballs were enucleated and divided randomly into four groups, with 20 eyeballs in each group. The eyeballs in each group were respectively fixed in Fixative 1 (4 g paraformaldehyde were added into 0.1 mol/L phosphate buffer 80 mL, heated to 60 ℃, agitated still the powder were dissolved, then clarified with 1 mol/L NaOH. When it was cooled down, 5 mL glacial acetic acid and 10 mL acetone were added, and 0.1 mol/L phosphate buffer was added to make up the volume to 100 mL), Fixative 2 (5% potassium dichromate solution 40 mL, 4% formaldehyde 10 mL, glacial acetic acid 10 mL, trichloroacetic acid solution 10 mL, 10% sodium acetate trihydrate solution 10 mL), Fixative 3 (glacial acetic acid 10 mL, 40% formaldehyde 20 mL, physiological saline 70 mL, 75% alcohol 10 mL), and Fixative 4 (alcohol 60 mL, 40% formaldehyde 10 mL, glacial acetic acid 10 mL, chloroform 20 mL). After 24 hours of fixation, paraffin section and hematoxylin-eosin staining were performed.
    RESULTS AND CONCLUSION: The retina fixed by Fixative 4 (alcohol 60 mL, 40% formaldehyde 10 mL, glacial acetic acid 10 mL, chloroform 20 mL) gave us a clear view of every layer of the retina, and retinal detachment was rarely seen. Fixative 4 promotes the permeation into the eyeballs, reduces the incidence of retinal detachment, and is better than the other fixatives.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Three-dimensional growth of adipose-derived stem cells in bone tissue engineering complex
    Wang Jian-wu, Dang Jian-jun, Li Qiang, Li Wen-gao, Zheng Yu
    2014, 18 (24):  3797-3802.  doi: 10.3969/j.issn.2095-4344.2014.24.005
    Abstract ( 304 )   PDF (2177KB) ( 421 )   Save

    BACKGROUND: Tissue engineering scaffolds are designed to imitate extracellular matrix, which is essential for cellular growth and metabolism. The scaffold preparation and the selection of seed cells are the hot topics in bone tissue engineering field.
    OBJECTIVE: To construct three-dimensional growth environment of adipose-derived stem cells with the chitosan/xenogeneic bone, and investigate its compatibility. METHODS: Adipose tissue was obtained from New Zealand white rabbits at 8 days old, and adipose-derived stem cells were extracted. After induction of osteogenic differentiation in vitro, the cells were cultured on chitosan/xenogenic bone, to construct bone tissue engineering complex, serving as the experimental group. The adipose-derived stem cells were directly cultured in xenogenic bone, to construct cells/xenogeneic bone complex, serving as a control group. The simple bone xenograft served as a blank group. Two weeks after in vitro induced differentiation, the complex or xenograft in three groups were observed under scanning electron microscope to observe the cells and scaffold compound the situation.
    RESULTS AND CONCLUSION: The chitosan fully penetrated into the scaffolds, and formed a three-dimensional environment for cell growth, so that adipose-derived stem cells can grow in the three-dimensional environment, which provide sufficient space for the regeneration of the extracellular matrix. After induction of the chitosan/xenogenic bone, adipose-derived stem cells can carry more cells, and the loss of cells in the vector is reduced, so it is a good carrier of bone tissue engineering.



    中国组织工程研究
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    Bone metabolism in patients with osteoporotic vertebral deformation
    Gao Fei, Qiao Ju-feng, Gao Feng, Tang Qi, Mo Ji-xian, Li Ming-zhang
    2014, 18 (24):  3803-3807.  doi: 10.3969/j.issn.2095-4344.2014.24.006
    Abstract ( 517 )   PDF (555KB) ( 511 )   Save

    BACKGROUND: Osteoporosis often leads to thoracolumbar deformation, since bone metabolism indicators are sensitive to reflect conversion process of individual bone, it can reduce misdiagnosis and predict fracture risk when combined with bone mineral density, thus early reducing the incidence of severe vertebral deformity.
    OBJECTIVE: To analyze the significance of bone metabolism in patients with osteoporotic vertebral deformation.
    METHODS: A total of 157 elderly patients with osteoporosis were divided into vertebral deformation group and normal vertebrae group according to the presence of vertebral deformation. In the vertebral deformation group, the patients were further assigned into normal bone mineral density subgroup and reduced bone mineral density subgroup. Bone mineral density and N-telopeptides of type I collagen, β-Crosslaps, osteocalcin were tested in each group. Finally we compared the differences of bone metabolism between each group.
    RESULTS AND CONCLUSION: N-telopeptides of type I collagen, β-Crosslaps and osteocalcin in vertebral deformation group were higher than that in normal group (P < 0.05). N-telopeptides of type I collagen and β-Crosslaps in normal bone mineral density subgroup were higher than that in reduced bone mineral density subgroup (P < 0.05), there was no significant difference between the two subgroups (P > 0.05). The results showed that vertebral deformation group has a higher level of bone metabolism than normal group, normal bone mineral density subgroup expresses higher levels of N-telopeptides of type I collagen and β-Crosslap than reduced bone mineral density subgroup in vertebral deformation group. The higher level of bone metabolism combined with bone mineral density and vertebral deformation can contribute to diagnose osteoporosis and predict vertebral fragile fractures.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Transforming growth factor-beta 1 expression in early enthesiopathy of Achilles tendon in rats after low-level laser therapy
    Shi Xiao-wei, Long Li-juan, Shen Yong-wei, Wang Zhi-peng
    2014, 18 (24):  3808-3813.  doi: 10.3969/j.issn.2095-4344.2014.24.007
    Abstract ( 237 )   PDF (742KB) ( 497 )   Save

    BACKGROUND: Low-level laser is a kind of therapeutic laser and has great potential application value in the field of sports medicine.
    OBJECTIVE: To discuss the repairing mechanism of low-level laser therapy in early enthesiopathy of achilles tendon in rats through observing the change of transforming growth factor-β1 expression after low-level laser therapy.
    METHODS: A total of 96 male Wistar rats aged 4 weeks were randomly divided into control group (n=8) and model group (n=88). Then model group was assigned randomly into two subgroups: model control group (n=48) and laser groups (n=40). The laser group was further allocated to 1, 2, 3, 7, 14 days of laser radiation, with eight rats in each. The rats of model group were trained by electrical stimulation for 5 weeks, and the rats of laser groups received laser radiation. The expression of transforming growth factor-β1 mRNA level in Achilles tendon was analyzed by real-time quantitative PCR. The transforming growth factor-β1 content was tested by double antibody ABC-ELISA.
    RESULTS AND CONCLUSION: The transforming growth factor-β1 in model control group was increased significantly compared with control group immediately after modeling (P < 0.05). Then, transforming growth factor-β1 was gradually decreasing as the time prolonged. Compared with laser groups, transforming growth factor-β1 in model control group was decreased significantly at 7 and 14 days (P < 0.05). Low-level laser therapy promotes the early healing of achilles tendon enthesiopathy in rats, delays the decrease of transforming growth factor-β1 content, and maintains high level of transforming growth factor-β1 at 7-14 days. Low-level laser therapy plays an important role in regulating extracellular matrix.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Prophylactic effects of edaravone on the extremity after limb replantation
    Sun Hai-bo, Wei Jun-qiang, Chen Wei, Zhang Ying-ze, Zheng Zhan-le, Pan Jin-she
    2014, 18 (24):  3814-3820.  doi: 10.3969/j.issn.2095-4344.2014.24.008
    Abstract ( 337 )   PDF (2507KB) ( 387 )   Save

    BACKGROUND: At present, there is no effective method to attenuate the severity of ischemia/reperfusion injury after limb replantation.
    OBJECTIVE: To investigate the prophylactic effects of edaravone solution on extremity after limb replantation.  
    METHODS: According to different interventions, 18 healthy rabbits were randomly divided into three groups (n=6): edaravone group, control group and ischemia/reperfusion injury group. The replantation models in the left hindlimbs were established. In edaravone group, rabbits were perfused with edaravone solution (1.5 mg/kg) before the reperfusion. At 2, 4, 8, 12 hours, the tibialis anterior muscles tissues of rabbits were harvested, and the following levels were determined, including superoxide dismutase, myeloperoxidase, malondialdehyde, Ca2+-ATPase, Na+-K+-ATPase, wet weight/dry weight ratio. The morphology of tibialis anterior muscles was observed under light microscope. 
    RESULTS AND CONCLUSION: After ischemia and reperfusion, the skeletal muscle fibers appeared edema and apparent damage, a large number of inflammatory cells infiltrated. After the intervention of edaravone solution, the damage was attenuated, but aggravated along the time. Edaravone solution significantly increased the level of superoxide dismutase, myeloperoxidase, Ca2+-ATPase, Na+-K+-ATPase, and significantly decreased the level of malondialdehyde and wet weight/dry weight ratio (P < 0.05). Edaravone solution exerts apparent protective effects on extremity after limb replantation, and can improve the ischemia/reperfusion injury in the extremity (especially skeletal muscle) after limb replantation.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Bone morphogenetic protein-7 inhibits apoptosis of human nucleus pulposus cells through activation of PI3K/Akt pathway
    Yu Hao-tao, Xu Yi-chun, Wang Qi-you, Ou Ding-qiang, Zhou Wei
    2014, 18 (24):  3821-3828.  doi: 10.3969/j.issn.2095-4344.2014.24.009
    Abstract ( 396 )   PDF (2639KB) ( 571 )   Save

    BACKGROUND: Bone morphogenetic protein 7 (BMP-7) can promote extracellular matrix synthesis of human nucleus pulposus cells, and delay intervertebral disk degeneration. Recently scholars proposed that the above effects can be achieved through anti-apoaptosis, but further molecular mechanism remains poorly understood.
    OBJECTIVE: To observe the effects of BMP-7 on the apoptotic human nucleus pulposus cells under serum-free induction and the PI3K/Akt pathway, investigate the molecular mechanism underlying the anti-apoptosis effect of BMP-7.
    METHODS: Human nucleus pulposus tissue were harvested from 12 patients according to the modified Pfirrmann classification, and human nucleus pulposus cells were obtained from digestion of herniated nucleus  pulposus tissue. The collected nucleus pulposus cells were divided into four groups. Blank group: cells were cultured in normal culture medium containing 15% feral bovine serum; positive control group: cells were induced to apoptosis in the serum-free culture medium for 48 hours; treated group: cells were induced to apoptosis in the serum-free culture medium containing different dosages of BMP-7; antagonism group: cells were induced to apoptosis in the serum-free culture medium containing different dosages of BMP-7 and LY294002, an PI3K/Akt pathway inhibitor. Cellular apoptosis rate was quantified by flow cytometry. Immunoflurorescence was performed to observe p-Akt expressions. Changes of Akt, p-Akt, BAD, and Caspase 9 expression were detected with western blot analysis.
    RESULTS AND CONCLUSION: Results from flow cytometry showed that, the apoptosis rate of nucleus pulposus cells was significantly decreased as the increase of BMP-7 concentration, under the serum-free induction; after the cells were induced with LY294002, the apoptosis rate was increased again (P < 0.05). The p-Akt immunoflurorescence and western blot analysis demonstrated that, compared with positive control group, the p-Akt expression was significantly increased, while the expression of downstream apoptosis-related proteins such as BAD and Caspase 9, was significantly decreased in the cells treated with BMP-7 (P < 0.05). Furthermore, in the group treated with BMP-7 and LY294002, the p-Akt expression was decreased, while the expression of apoptosis-related proteins was recovered to high levels (P < 0.05). BMP-7 activates the PI3K/Akt pathway, suppresses the downstream BAD and Caspase 9 signaling, and inhibits the apoptosis of human nucleus pulposus cells induced by serum-free situation.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    T-type calcium channel in sinoatrial node cells of rats after repeated exhaustive exercise
    Bo Bing
    2014, 18 (24):  3829-3834.  doi: 10.3969/j.issn.2095-4344.2014.24.010
    Abstract ( 326 )   PDF (2076KB) ( 659 )   Save

    BACKGROUND: At present, there is little evidence about the effect of exercise on T-type calcium channel (T-Ca2+ channel) in sinoatrial node cells.
    OBJECTIVE: To analyze the subunit Cav3.1 mRNA and current density of T-Ca2+ channel of sinoatrial node cells after 2 weeks of repeated exhaustive treadmill exercise in rats.
    METHODS: Fifty healthy male Sprague-Dawley rats were equally assigned into five groups,including one control groups (n=10; no exercise), two exhaustive exercise groups (n=20; single treadmill running at 25 m/min, 0° slope), and two repeated exhaustive exercise groups (n=20; repeated treadmill running at 25 m/min, 0° slope, once per day, 6 days per week, for 2 weeks. The samples were drawn from 10 rats in the exercise groups at 0 and 24 hours after exhaustive treadmill running respectively. The mRNA expression of T-Ca2+ channel subunit Cav3.1 in sinoatrial node was analyzed by real-time fluorescent quantitative PCR. The current density of T-Ca2+ channel was analyzed with acute isolation and whole-cell patch clamp techniques, to observe the effect of repeated treadmill exercise on T-Ca2+ channel.
    RESULTS AND CONCLUSION: The Cav3.1 mRNA expression of the repeated exhaustive exercise groups at 0 and 24 hours were significantly lower than that of control group (P < 0.01). The current density of T-Ca2+ channel  of the repeated exhaustive exercise groups at 0 and 24 hours were significantly lower than that of control group and single exhaustive exercise group (P < 0.01, P < 0.01). Two-week repeated exhaustive treadmill exercise can decrease the subunit Cav3.1 mRNA expression and the current density of T-Ca2+ channel in the sinoatrial node cells. The exhaustive exercise induced diastolic automatic depolarization and slowed down spontaneous activity of sinoatrial node cells, which may be one of the most important mechanism of exercise-induced sinoatrial node dysfunction and arrhythmia.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Changes of bone mass after immediate implant in maxillary anterior region: whether horizonal resorption affects the osseointegration of implants
    Ye Bin, Lin Zhen-yan, Xie Li-li, Sun Lei, Gao Yong-bo
    2014, 18 (24):  3835-3839.  doi: 10.3969/j.issn.2095-4344.2014.24.011
    Abstract ( 538 )   PDF (616KB) ( 660 )   Save

    BACKGROUND: Currently a large number of animal experiments and clinical studies have confirmed that, both immediate implants and delayed implants achieve successful osseointegration, but whether immediate implants can reduce or prevent physiological bone resorption of extracted teeth crypt alveolar crest has been the focus of debates among scholars.
    OBJECTIVE: To evaluate the recent changes of immediate implant bone in anterior maxilla by the use of cone-beam CT.
    METHODS: Eighteen patients with 18 maxillary anterior teeth that can not be reserved were treated with immediate implantation, and were included in the study. On the surgery day, postoperative 6 months and 1 year, the patients were detected with cone-beam CT. The bone wall thickness at the lingual side of alveolar crest at   4 mm, 6 mm, 8 mm away from the implant shoulder was measured. The bone height at the lingual-buccal side of missing teeth alveolar crest was also recorded.
    RESULTS AND CONCLUSION: At 6 months after surgery, the bone height at the lingual-buccal side of missing teeth alveolar crest was (1.83±0.05) mm and (1.50±0.04) mm, the bone resorption at the lingual side of alveolar crest at 4 mm, 6 mm, 8 mm away from the implant shoulder was (1.72±0.30) mm, (1.65±0.26) mm, (1.55±0.25) mm, respectively. At 1 year, the bone height at the lingual-buccal side of missing teeth alveolar crest was (0.85± 0.04) mm and (0.78±0.05) mm, the bone resorption at the lingual side of alveolar crest at 4 mm, 6 mm, 8 mm away from the implant shoulder was (0.52±0.20) mm, (0.45±0.16) mm, (0.32±0.15) mm, respectively. Immediate implant may induce horizontal resorption at the lingual side bone wall, but has no impact on the osseointegration of the implant, and the bone resorption is stable 1 year later.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Geometric accuracy of magnetic resonance imaging displaying the inferior alveolar nerve
    Deng Wei, Huang Dai-ying, Chen Song-ling, Chen Jing-xin, Liao Tian-an
    2014, 18 (24):  3840-3844.  doi: 10.3969/j.issn.2095-4344.2014.24.012
    Abstract ( 405 )   PDF (646KB) ( 528 )   Save

    BACKGROUND: MRI makes up for a deficiency of X-ray and CT images in nerve imaging and can display inferior alveolar nerve clearly and directly.
    OBJECTIVE: To assess the geometric accuracy of MRI displaying the inferior alveolar nerve.
    METHODS: One fresh human cadaver head was scanned by MRI and CT after removing the soft tissue. The distances were measured between the second premolar and the first molar, the first molar and the second molar, the second molar and the third molar. After cutting the bilateral mandible on the same sites with a saw, the distances between inferior alveolar nerve and cortex were measured with a caliper. The data measured by MRI, CT and caliper were statistically analyzed for the t-test.
    RESULTS AND CONCLUSION: The average distances measured by the three methods between inferior alveolar nerve and cortex were (4.894±1.692), (4.906±1.715) and (4.861±1.718) mm, respectively. There was no significant difference in the average distance among the three methods (P > 0.05). The geometric accuracy of MRI displaying the inferior alveolar nerve was good. It can provide experimental evidence for MRI application in preoperative imaging such as dental implant.



    中国组织工程研究
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    Maxillary first molar displacement and stress analysis of transmission straight wire technique under gable bend effect
    Ding Rui, Bai Xin-hua, Man Yun-na, Wu Pei-ling, Wang Wei
    2014, 18 (24):  3845-3849.  doi: 10.3969/j.issn.2095-4344.2014.24.013
    Abstract ( 541 )   PDF (770KB) ( 432 )   Save

    BACKGROUND: With the development of modern orthodontics techniques, designing an efficient appliance is the focus in recent studies. Transmission straight wire appliance was invented under this background. This appliance can accelerate teeth leveling, shorten treatment duration, improve correction efficacy, and provide a novel approach to solve intractable cases. The relationship between maxillary first molar displacement and stress distribution needs further study.
    OBJECTIVE: To establish three-dimensional finite element model with better biological and mechanical similarity of transmission straight wire appliance, and obtain the relationship between maxillary first molar displacement and stress distribution.
    METHODS: By using 64-row spiral CT scanning, the sectional image data in DICOM of TMJ, maxilla and upper tooth were acquired from a volunteer with Angle Class II division 1. With the help of Mimics 10.01, Geomagic Studio 8.0, Unigraphics NX 6.0 and Ansys workbench 13.0 softwares, the three-dimensional finite element model of transmission straight wire appliance, bend, Australian Orthodontic Wire, including maxilla, upper tooth, periodontal ligament, arch wire and brackets was established in Windows XP Service Pack 3 system. The model was loading, to obtain the maxillary first molar displacement and stress distribution.
    RESULTS AND CONCLUSION: A three-dimensional finite element model of transmission straight wire appliance was established, which consisted of 250 929 elements and 657 766 nodes. Furthermore, the stress distribution of maxillary first molar tilt displacement trend curve under the action of maxillary first molar region was observed, which favors to analyze transmission straight wire appliance mechanical system and guide the clinical application and appliance improvements. Our findings indicate that, under the tilt bending, dental root will be close to the buccal cortical bone, leading to stress concentration at the root tip and maxillary buccal cortical areas; this stress concentration may lead to root resorption of the maxillary first molar.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    MRI measurement of position, orientation and shape of long biceps tendon in healthy volunteers
    Zhao Heng, Liu Jin-cai, Chen Li-xun, Zhou Hong, Hu Rong, Qing Wei-peng, Quan Xiao-bing
    2014, 18 (24):  3850-3854.  doi: 10.3969/j.issn.2095-4344.2014.24.014
    Abstract ( 498 )   PDF (593KB) ( 512 )   Save

    BACKGROUND: In previous studies, the eccentricity of long biceps tendon at the entrance to the groove has not been measured, but merely described qualitatively.
    OBJECTIVE: To characterize the position, orientation and shape of the long biceps tendon on MR images acquired in neutral position, external rotation, and internal rotation of the shoulder in asymptomatic volunteers, to explore MRI characteristics of the long biceps tendon for clinical assessment.
    METHODS: A total of 35 asymptomatic volunteers were included. MR scanning images were acquired in neutral position, external rotation and internal rotation of the shoulder. All MR images were evaluated by two radiologists, exclusive criteria were volunteers with lesions or any kind of degenerative changes to the rotator cuff tendons, coracohumeral ligament, superior glenohumeral ligament, and/or pulley fibers. The 3D WATSc sequence of long biceps tendon was measured at superior, middle, and inferior levels of the bicipital groove by a radiologist. The position, orientation, and shape of long biceps tendon were measured.
    RESULTS AND CONCLUSION: The results of MR images in neutral position, external rotation and internal rotation of the shoulder were followed: (1) The long biceps tendon eccentricity was maximal in the neutral position. (2) The orientations of long biceps tendon were acute angle, and the differences in long biceps tendon orientation were significant at middle and inferior measurement levels. (3) At inferior measurement level, the changes of shape of long biceps tendon showed significant differences. The findings showed that the position, orientation and shape of the long biceps tendon were slightly dependent on shoulder rotation and measurement level.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Surface electromyography of neck shoulder muscles in patients with neck and shoulder pain
    Huang Ping, Qian Nian-dong, Qi Jin, Luo Shi-hua, Chen Bo, Deng Lian-fu
    2014, 18 (24):  3855-3862.  doi: 10.3969/j.issn.2095-4344.2014.24.015
    Abstract ( 746 )   PDF (866KB) ( 870 )   Save

    BACKGROUND: Pain of neck and shoulder is the common clinical symptoms caused by chronic strain of the neck and shoulder soft tissue (mainly muscle). Surface lectromyography is a new noninvasive examination method for muscle activity, which can measure the muscle activity and function.
    OBJECTIVE: To evaluate the neck-shoulder muscle function in patients with neck and shoulder pain, and provide theoretical basis for the application of surface electromyography and the reasonable prevention and rehabilitation of neck and shoulder pain.
    METHODS: Thirty-two office staffs with unilateral neck and shoulder pain were examined with surface electromyography, and the surface electromyographies of the neck erector muscle and trapezius muscle were recorded under the posture of lowering head, lifting head, and hands up. The visual analogue scales of neck and shoulder pain were recorded before the test. Then the data were collected, and the muscle electromyography activities were compared between the pain side and non-pain side.
    RESULTS AND CONCLUSION: The average visual analogue scales in patients with neck and shoulder pain was 5.03 before test. Under different postures, the original myoelectricity signals of the neck erector muscle and  trapezius muscle in the pain side were weaker than that in the non-pain side; there were significant differences in the average amplitude value of the neck erector muscle and trapezius muscle between the pain side and the non-pain side (P < 0.05). No significant difference was found in the average frequency slope value of the neck s erector muscle and trapezius muscle between the pain side and the non-pain side (P > 0.05). The results showed that, the neck and shoulder muscle activity ability decreased at the pain side of patients with neck and shoulder pain, and the office staffs with long-term seat assignments should exercise their neck and shoulder muscles regularly.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Nerve growth factor promotes the expression of vascular endothelial growth factor in the fracture healing process
    Liu Yu-peng, Zhao De-wei, Wang Wei-ming, Zhang Yao, Li Fang, Liu Zhen-gang
    2014, 18 (24):  3863-3869.  doi: 10.3969/j.issn.2095-4344.2014.24.016
    Abstract ( 305 )   PDF (333KB) ( 831 )   Save

    BACKGROUND: The fracture healing process is very complex, involving a variety of cytokines. At present, the existing studies focus on bone morphogenetic protein, fibroblast growth factor, transforming growth factor β, vascular endothelial cell growth factor and insulin-like growth factor. The role of nerve growth factor on vascular endothelial cell growth factor during the fracture healing process remains unclear.
    OBJECTIVE: To investigate the effect of nerve growth factor on vascular endothelial growth factor expression during the process of fracture healing in rabbits.
    METHODS: Radial fracture model established in white rabbits were divided into three groups, they were treated with nerve growth factor, nerve growth factor monoclonal antibody, and saline respectively, serving as nerve growth factor group, antagonizing nerve growth factor group, and control group.
    RESULTS AND CONCLUSION: The vascular endothelial growth factor expression was detected with western blot analysis at 24 hours, 48 hours, 1 week, 3 weeks, 6 weeks, 8 weeks post-injury. The results showed that the expression level was the highest in nerve growth factor group, then in control group, and the lowest in antagonizing nerve growth factor group (P < 0.05). Experimental findings suggest that, nerve growth factor can promote the expression of vascular endothelial growth factor in the fracture healing process.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Construction and identification of pIRES2-LIF-VEGF165 bicistronic eukaryotic expression vector
    Li Bing-nan, Li Wei-dong, Lin Jun-tang, Feng Hui-gen, Yuan Zhi-qing
    2014, 18 (24):  3870-3877.  doi: 10.3969/j.issn.2095-4344.2014.24.017
    Abstract ( 200 )   PDF (853KB) ( 431 )   Save

    BACKGROUND: Human leukemia inhibitory factor (LIF) and vascular endothelial growth factor 165 (VEGF165) play an important role in neuronal survival after spinal cord injuries. The clinical application of viral vector has a hidden danger, and eukaryotic expressing vector is a way to solve this question.
    OBJECTIVE: To construct and identify pIRES2-LIF-VEGF165 bicistronic eukaryotic expression vector.
    METHODS: LIF genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by direct PCR. Then LIF cDNA fragment was inserted into the multiple cloning sites of pIRES2-EGFP, to generate the bicistronic eukaryotic expression plasmid pIRES2-LIF-EGFP. The VEGF165 gene was obtained from pIRES2-VEGF165-EGFP plasmid by twin PCR, then VEGF165 cDNA fragment was cloned into the pIRES2-LIF-EGFP instead of EGFP to create a double gene co-expressing vector plasmid pIRES2-LIF-VEGF165. Then pIRES2-LIF-VEGF165 was applied to transfect HEK293 cells, and the co-expression of double genes was detected using RT-PCR and western blot analysis.
    RESULTS AND CONCLUSION: The DNA sequencing analysis demonstrated that the LIF and VEGF165 were exactly consistent with the sequence recorded in the GenBank. The size of LIF gene and VEGF165 gene was respectively was 609 bp and 576 bp. Enzyme digestion analysis indicated that LIF band was found in the double gene co-expressing vector pIRES2-LIF-VEGF165 by EcoRI/BamHI, IRES-VEGF165 fragmet was found by BamHI/NotI, and LIF-IRES-VEGF165 fragment was found by EcoRI/NotI. RT-PCR and western blot analysis showed that, after transfection, both LIF and VEGF165 mRNA and protein were expression in HEK293 cells. LIF and VEGF165 bicistronic eukaryotic expression vector was successfully constructed in this study.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Optimization of two-dimensional gel electrophoresis for synaptic proteomics
    Hu Yong-bo, Gao Li, Wang Dan, Zeng Zhong, Xu Chao-yi, Xiao Bing, Luo Fei-fei, Yang Zhi-yong, Zhou Qiang
    2014, 18 (24):  3878-3884.  doi: 10.3969/j.issn.2095-4344.2014.24.018
    Abstract ( 261 )   PDF (1149KB) ( 722 )   Save

    BACKGROUND: Two-dimensional electrophoresis (2-DE) is one of the most popular methods of protein separation in synaptic proteomic analysis. However, there was no agreed standard on selection of immobilized pH gradients (IPG) strips and concentration of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the research literature on 2-DE of synaptic proteins.
    OBJECTIVE: To optimize IPG strips and concentration of SDS-PAGE in 2-DE for synaptic proteomic analysis, and to get high-quality 2-DE maps of synaptic proteins.
    METHODS: Rat hippocampal synapses were adopted in this experiment to compare the effect of 2-DE, which were involved with IPG strips with the linear range of pH 5.0-8.0 and pH 3.0-10.0, IPG strips with the linear and nonlinear range of pH 3.0-10.0, as well as single 10% and 12% SDS-PAGE. Meanwhile, a computer-based online search of PubMed (1989/2013) and CNKI (1989/2013) was performed for the articles about 2-DE of synaptic proteins. The selection of IPG strips and concentration of SDS-PAGE in the research literature were  summarized and evaluated, and the distribution characteristics of the synaptic proteins in 2-DE maps of IPG strips with various pH range and SDS-PAGE gels with different concentration were summarized.
    RESULTS AND CONCLUSION: The results demonstrated that the synaptic proteins were well separated conveniently and obtained high quality 2-DE maps by nonlinear IPG strips range of pH 3.0-10.0 and single 10% SDS-PAGE gels. At the same time, it was also recommended that combination use of IPG strips range of pH 4.0-7.0 and pH 6.0-11.0, as well as linear gradient 9-16% SDS-PAGE gels. They were all suitable for 2-DE of synaptic proteins.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Clinical application of tissue-engineered keratoprosthesis
    Chen Jia-jia, Wang Wen-zhan, Zhu Yu
    2014, 18 (24):  3885-3893.  doi: 10.3969/j.issn.2095-4344.2014.24.019
    Abstract ( 464 )   PDF (312KB) ( 466 )   Save

    BACKGROUND: Keratoprosthesis is a special kind of refractive apparatus made of alloplasm shaping material, which is designed to replace the turbid cornea tissue. The visual acuity may be obtained after surgical implantation into the affected eyes.
    OBJECTIVE: To introduce the advance of keratoprosthesis research.
    METHODS: A computer-based online retrieval was performed among Wanfang and Medline databases, by using the key words of “keratoprosthesis, corneal transplantation, biocompatibility, tissue engineering” in English and Chinese. The animal experiment and clinical progress of artificial cornea transplantation, implantation and biocompatibility, complications were discussed.
    RESULTS AND CONCLUSION: A total of 204 articles were selected, according to the inclusion and exclusion criteria, 50 articles were included in this study. People have made great progress in the research and production of keratoprosthesis within recent years. There are many new ideas in material choosing, handling and designing ways of non-tissue-engineered keratoprosthesis. Meanwhile, the appearance of active tissue-engineered keratoprosthesis created a new method for the research and production of keratoprosthesis.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Progress in tissue-engineered trachea: breakthrough in cartilage and blood supply disorders
    Sun Yan-qi, Ma Jian-qun, Xu Hai
    2014, 18 (24):  3894-3899.  doi: 10.3969/j.issn.2095-4344.2014.24.020
    Abstract ( 282 )   PDF (638KB) ( 385 )   Save

    BACKGROUND: Tracheal damage and defects are commonly seen in clinical practice. It is difficult to performed tracheal anastomosis, which requires implantation of tissue-engineered trachea or artificial trachea to repair defects and improve its function. After implantation of tissue engineered trachea, the revascularization, immune rejection, infection, fibrous scar formation and rehabilitation function have been widely concerned.
    OBJECTIVE: To review the progress of tissue engineered trachea seed cells, scaffolds, chondroplasia and vascularization, as well as the factors affecting the growth of tissue engineered trachea.
    METHODS: First author searched PubMed database, CNKI and Google Scholars from January 2000 to April 2014, by using search terms of “Tissue engineering, Tracheal reconstruction, Trachea substitute, Scaffold revascularization, Seed cells, Transforming growth factor” in English and Chinese. A total of 140 articles relevant to tissue engineered trachea were screened, and 45 papers of them met the inclusion criteria.
    RESULTS AND CONCLUSION: Tissue engineered trachea depends on the body’s living tissue or stem cells, which amplify and prolfierate on in vitro seed scaffolds, it is a technical method that repairs primary tracheal defects or in situ value-added end of the differentiation-inducing defects, thereby repairing the defect and achieve certain functions. The main factor of tissue engineered trachea is seed stem cells, tracheal scaffold, cartilage formation, vascular factors and growth factors that promote the regeneration of tissue engineered trachea.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    New progress of periodontal tissue engineering: problems and applications
    Lu Bo-wen, Xu Lu-lu, Zhang Yang, Han Guang
    2014, 18 (24):  3900-3905.  doi: 10.3969/j.issn.2095-4344.2014.24.021
    Abstract ( 300 )   PDF (682KB) ( 425 )   Save

    BACKGROUND: Tissue engineering technology provides novel methods for the regeneration of periodontal tissue.
    OBJECTIVE: To summarize the recent progress of periodontal tissue engineering seed cells, growth factor and scaffold materials.
    METHODS: The first author searched the PubMed database (http://www.ncbi.nlm.nih.gov/pubmed) on periodontal tissue engineering study, using the key words of periodontal tissue engineering in English; at the same time CBM database from January 2009 to December 2013 was also retrieved for periodontal tissue engineering literature, using the key words of periodontal tissue engineering in Chinese. Repetitive studies were excluded, and finally  30 articles were included to review.
    RESULTS AND CONCLUSION: Study showed that, periodontal ligament stem cells have good application prospect in periodontal tissue regeneration; bone marrow stromal stem cells is the ideal seed cells for the repair of periodontal tissue defects; dental follicle cells have pluripotent differentiation potentials and they can form periodontal tissue. A variety of biologically active molecules have been shown to have a strong function of repairing periodontal tissue, the growth factors associated with periodontal tissue engineering are mainly platelet-derived growth factor, bone morphogenetic protein, transforming growth factor β, insulin-like growth factor, basic fibroblast growth factor, and enamel matrix derivative. Currently the scaffold materials used in periodontal tissue engineering can be divided into natural biological materials and artificially synthetic materials. How to choose the best seed cells, cytokines and scaffold complexes needs to be further improved.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Cytokines and acupuncture in the treatment of knee osteoarthritis: experiments and applications
    Zhang Yong-liang, Mi Yi-qun, Gang Jia-hong, Wang Wen-hui
    2014, 18 (24):  3906-3911.  doi: 10.3969/j.issn.2095-4344.2014.24.022
    Abstract ( 376 )   PDF (687KB) ( 405 )   Save

    BACKGROUND: Studies have shown that acupuncture treatment have some effects in knee osteoarthritis.
    OBJECTIVE: To review the cytokines studies of acupuncture treatment on knee osteoarthritis.
    METHODS: A computer-based retrieval of PubMed, WOK, CNKI, VIP, Wanfang databases was performed to find the literatures on acupuncture treatment of knee osteoarthritis. All data were primarily screened to exclude irrelevant literature. Those literatures about the cytokines related to knee osteoarthritis were included. Repetitive studies and untypical study reports were excluded.
    RESULT AND CONCLUSION: Totally 48 articles were collected, including 31 in Chinese and 17 in English. The analysis results showed that, the cytokines associated with knee osteoarthritis can be substantially divided into destructive cytokines and protective cytokines. Destructive cytokines include matrix metalloproteinase, interleukin, tumor necrosis factor-α, prostaglandin E2, nitrogen monoxidum, and cartilage oligomeric matrix protein. Protective cytokines include bone morphogenetic protein, transforming growth factor-β, osteoprotegerin, insulin-like growth factor-1, and basic fibroblast growth factor. Many experimental studies have shown that acupuncture can reduce the levels of destructive cytokines such as matrix metalloproteinase, interleukin, tumor necrosis factor-α, prostaglandin E2, nitrogen monoxidum, and cartilage oligomeric matrix protein, and adjust the matrix metalloproteinase 1/tissue inhibitor of metalloproteinase 1 ratio imbalances, improve the levels of protective cytokines such as bone morphogenetic protein, transforming growth factor-β, osteoprotegerin, insulin-like growth factor-1, and basic fibroblast growth factor. Therefore acupuncture plays the role of anti-inflammatory, preventing the degeneration of articular cartilage, and promoting cartilage tissue repair.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Progress of proteomics technology and its application in osteoarthritis
    Pan Ding, Lei Guang-hua
    2014, 18 (24):  3912-3918.  doi: 10.3969/j.issn.2095-4344.2014.24.023
    Abstract ( 313 )   PDF (820KB) ( 670 )   Save

    BACKGROUND: Proteomics research has thoroughly reformed the way to study the diseases, and provides the understanding of the mechanism of osteoarthritis pathological physiology.
    OBJECTIVE: To focus on the research progress of proteomics technology in osteoarthritis early diagnosis, therapeutic effect and pathogenesis molecular markers.
    METHODS: Researches on proteomics technology and osteoarthritis published in journals were identified manually and on-line retrieved by using SinoMed, Chongqing VIP database, Wanfang Data and CNKI database. Those researches reported in English journals were identified using MEDLINE, Elsevier: ScienceDirect, and BIOSIS Previews database. Selected studies should describe a related and reliable study defined by strict screening and diagnostic criteria.
    RESULTS AND CONCLUSION: Proteomics technology for the osteoarthritis in early diagnosis, treatment effect and pathogenesis molecular markers has achieved a certain progress. Research showed that proteomics has great application value in screening osteoarthritis molecular markers, and has the characteristics of high flux and high speed compared with single protein identification, so it plays an important role in the diagnosis of osteoarthritis and drug target identification. Proteome database is a sign of proteome research level. The development of the bioinformatics has provided a more convenient and effective computer analysis software for proteomic study.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Biomechanical risk factors and prevention of non-contact anterior cruciate ligament injury
    Zheng Rong-qiang, Zhou Jing-yi
    2014, 18 (24):  3919-3924.  doi: 10.3969/j.issn.2095-4344.2014.24.024
    Abstract ( 303 )   PDF (578KB) ( 776 )   Save

    BACKGROUND: Non-contact anterior cruciate ligament injury accounts for about 80% of all anterior cruciate ligament injuries, and mostly occurs in adolescent females. How to assess risk factors of the injury and screen in high-risk populations is essential for effective injury prevention.
    OBJECTIVE: To summarize the relevant studies about the biomechanical risk factors and preventions methods of non-contact anterior cruciate ligament injury, and open up new ideas for the prevention of non-contact anterior cruciate ligament injury.
    METHODS: The PubMed database and CNKI database were retrieved for articles published from 1979 to 2014 by network. The English key words were “non-contact anterior cruciate ligament, biomechanicas, risk factors” and “non-contact anterior cruciate ligament, prevention”, while the Chinese key word was “anterior cruciate ligament injury, biomechanics”. The key words were put in the titles and abstracts to search the articles. A total of 58 articles were included which related to biomechanics research and prevention of anterior cruciate ligament injury.
    RESULTS AND CONCLUSION: Biomechanical risk factors can be evaluated among adolescents who participate in sports. Landing quickly, stopping, step cutting and other high-risk actions were screened and analyzed. High-risk crowd of non-contact anterior cruciate ligament injury can be found, and then targeted training. Prophylactic training can improve exercise performance, jumping height and the stability of knee joint in dynamic loading conditions, develop the balance, and increase hamstring muscle strength and controlling ability, which can maximize the prevention of non-contact anterior cruciate ligament injuries.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effects of bacterial biofilm on chronic wound healing and its treatment strategy
    Zeng Yan-ni, Huang Jin, Qiu Tie-ying
    2014, 18 (24):  3925-3930.  doi: 10.3969/j.issn.2095-4344.2014.24.025
    Abstract ( 848 )   PDF (668KB) ( 1762 )   Save

    BACKGROUND: Understanding the formation of bacterial biofilm and the mechanism of bacterial biofilm on wound healing, and preventing the influence of bacterial biofilm on wound healing, play a crucial role on improving the healing rate of chronic wounds.
    OBJECTIVE: To review the mechanism of bacterial biofilm on wound healing, and the current nursing strategy.
    METHODS: A computer-based retrieval of Springerlink and Chinese National Knowledge Infrastructure (CNKI) database was performed for articles published between 2008 and 2013. The key words are “bacterial biofilm, chronic wound infection” in English and Chinese. Articles about the wound infection nursing and bacterial biofilm treatment were reviewed.
    RESULTS AND CONCLUSION: Growing evidence have shown that, pathogenic bacteria or opportunistic pathogen caused chronic wound infection is related to the formation of bacterial biofilm, which is one of the most important reasons of chronic wound non-healing. The mechanism of the bacterial biofilm formation is complicated. Standard procedure, environmental management, rational use of antibiotics, timely debridement can prevent the formation of bacterial biofilm. Treatment methods for preventing the formation of bacterial biofilm include ultrasonic treatment, negative pressure treatment, phage therapy, lactoferrin, gallium nitrate, ethylenediamine tetraacetic acid, newtol, bee honey, traditional Chinese medicine, hyperbaric oxygen therapy and so on.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Antler regeneration and its proteomics research
    Dong Zhen, Chu Wen-hui, Zhao Hai-ping, Liu Zhen, Wang Gui-wu, Li Chun-yi
    2014, 18 (24):  3931-3936.  doi: 10.3969/j.issn.2095-4344.2014.24.026
    Abstract ( 300 )   PDF (698KB) ( 699 )   Save

    BACKGROUND: Deer antlers are the only known mammalian organs that can periodically regenerate, and antler regeneration is known as a stem cell-based process. The stem cells locate in the potentiated region of pedicle periosteum, and form the basis for deer antler regeneration. Study on the proteome in deer antler and pedicle periosteum is of great importance for revealing biological activity and the mechanism underlying deer antler regeneration.
    OBJECTIVE: To summarize research progress in antler regeneration, as well as the major approaches and the status of antler proteomics research.
    METHODS: A computer-based online search of PubMed and CNKI databases was performed for acquiring the articles in English and Chinese by using the key words of “deer antler; antler regeneration; antler proteome”. The searched articles are all related with antler regeneration histology and morphology, antler stem cells and proteome studies. Repetitive studies or articles that are unrelated to the search criteria were excluded. A total of 43 papers were included for review.
    RESULTS AND CONCLUSION: After preliminary screening in PubMed and CNKI database, 43 articles were finally selected. The literature showed that, based on the histological experiments, deer antlers can periodically regenerate and antler regeneration is a stem cell-based process. The pedicle periosteum is divided into potentiated region and dormant region, which are distinguished by the degree of contacting with the skin. The interactions between potentiated region and its covering skin prompts antler stem cells to develop into a complete antler. And the proteomes in antler and pedicle periosteum play important roles in regulating the regeneration. Getting a complete protein map and using genetic engineering to study the function of unknown proteins can establish significant foundation for understanding unique biological activities and regeneration of deer antler. And it can provide a reference in the regulation effect of related proteins in the regenerative mammalian organs.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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