Micro CT study on trabecular microstructure of tibial plateau in ovariectomized goats

doi:10.3969/j.issn.2095-4344.2014.24.001

Abstract

Abstract:

BACKGROUND: Trabecular microstructure of tibial plateau changes significantly under the progress of osteoporosis. Micro CT is a comprehensive, three-dimensional, noninvasive technology, which provides new method of measuring bone microstructure, evaluating bone quality and predicting bone strength. In recent years, this technique has been widely used in the field of osteoporosis study.
OBJECTIVE: To investigate the trabecular microstructure of tibial plateau in ovariectomized goats by Micro CT.
METHODS: Twelve female goats at 2.5 years old were randomly divided into ovariectomy (OVX) and sham groups, and each group included six goats. Goats in OVX group underwent ovarian surgery, while sham-operated goats removed equal amount of abdominal fat tissue. Half of goats in both OVX and sham groups were executed at 2 and 4 years after surgery, respectively. The left tibial plateau was harvested and a high resolution Micro CT was used to identify the trabecular microstructure parameters of metaphysis and epiphysis.
RESULTS AND CONCLUSION: Compared with the sham-operated goats at 2 and 4 years post-surgery, bone volume fraction, trabecular number and trabecular thickness were decreased in the OVX group (P < 0.05), while trabecular separation was increased (P < 0.05), which all appeared a time-dependent manner. As exceptions, for the comparison of trabecular thickness in epiphysis between OVX group and sham group at 4 years post-surgery (P > 0.05), and for the comparison of both trabecular thickness and trabecular separation in epiphysis at 2 years post-surgery (P > 0.05), there was no significant difference. At 2 and 4 years after surgery, microstructure parameters in metaphysis changed more obviously than them in epiphysis. The trabecular microstructure showed the differences between metaphysis and epiphysis of tibial plateau. The change of trabecular microstructure appears to be site-specific in the progress of osteoporosis in tibial plateau, and the microstructure deterioration of trabecular bone in the metaphysis of tibial plateau happens severer than that in the epiphysis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: osteoporosis, ovariectomy, tibia, microstructure, bone mineral density

CLC Number:

R318

Wang De-zhi, Chen Shi-chang, Liang Zheng-yang, Wang You. Micro CT study on trabecular microstructure of tibial plateau in ovariectomized goats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(24): 3773-3778.

Figures/Tables 4

References

[1] 李杨,冯世庆,杨宁,等.局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应[J].中国组织工程研究,2013,17(46): 7994-7999.
[2] 王新祥,张允岭,吴坚.葛根对睾丸切除骨质疏松模型小鼠骨密度和骨构造的作用[J].中国组织工程研究与临床康复,2010,14(7): 1262-1266.
[3] 韩亚军,帖小佳,伊力哈木•托合提.中国中老年人骨质疏松症患病率的Meta分析[J].中国组织工程研究,2014,18(7):1129-1134.
[4] 赵玺,赵文,孙璟,等.骨代谢指标与骨关节炎及绝经后骨质疏松症的关系[J].中国组织工程研究,2014,18(2):245-250.
[5] Raafat BM, Hassan NS, Aziz SW, et al. Bone mineral density (BMD) and osteoporosis risk factor in Egyptian male and female battery manufacturing workers. Toxicol Ind Health. 2012;28(3):245-52.
[6] Blake GM, Griffith JF, Yeung DK, et al. Effect of increasing vertebral marrow fat content on BMD measurement, T-Score status and fracture risk prediction by DXA. Bone. 2009;44(3): 495-501.
[7] McNamara LM. Perspective on post-menopausal osteoporosis: establishing an interdisciplinary understanding of the sequence of events from the molecular level to whole bone fractures. J R Soc Interface. 2010;7(44):353-372.
[8] 张钧,江莉婷,王晋申,等.1型糖尿病小鼠下颌骨三维结构及组织形态[J].中国组织工程研究,2013,17(28):5101-5107.
[9] 余文超,刘岩,袁文.脊髓损伤早期及制动大鼠股骨干骺端的显微CT观察[J].中国组织工程研究与临床康复,2010,15(30): 5596-5599.
[10] Effendy NM, Khamis MF, Shuid AN. Micro-CT assessments of potential anti-osteoporotic agents. Curr Drug Targets. 2013; 14(13):1542-1551.
[11] 王亮,张志敏,甄相周,等.去势雌性山羊骨质疏松模型的特点[J].中国组织工程研究,2012,16(7):1303-1306.
[12] 李素萍.骨质疏松动物模型的研究现状[J].中国组织工程研究与临床康复,2011,15(20):3767-3770.
[13] Newton B, Cooper RC, Gilbert JA, et al. The ovariectomized sheep as a model for human bone loss. J Comp Pathol. 2004; 130(4):323.
[14] Oheim R, Amling M, Ignatius A, et al. Large animal model for osteoporosis in humans: the ewe. Eur Cell Mater. 2012;24: 372-385.
[15] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30.
[16] 丁文鸽.卵巢切除骨质疏松小鼠骨折愈合不同时期骨微结构及力学性能变化[J].中国组织工程研究与临床康复, 2008,12(42): 8247-8250.
[17] Davison KS, Kendler DL, Ammann P, et al. Assessing fracture risk and effects of osteoporosis drugs:bone mineral density and beyond. Am J Med. 2009;122(11):992-997.
[18] Rubin CD. Emerging concepts in osteoporosis and bone strength. Curr Med Res Opin. 2005;21(7):1049-1056.
[19] 李冠武,汤光宇.Micro-CT及1H-MRS在骨质疏松骨质量研究中的应用[J].国际医学放射学杂志,2010,33(6):525-528.
[20] 粱敏,张劼,粱杏欢,等.雌孕激素联合对去卵巢大鼠骨密度和骨形态计量学的影响[J].中国组织工程研究与临床康复, 2010, 14(24): 4380-4384.
[21] Kim JE, Shin JM, Oh SO, et al. The three-dimensional microstructure of trabecular bone:Analysis of site-specific variation in the human jaw bone. Imaging Sci Den. 2013; 43(4):227-233.
[22] Chen H, Wu M, Kubo KY. Combined treatment with a traditional Chinese medicine, Hachimi-jio-gan (Ba-Wei-Di-Huang-Wan) and alendronate improves bone microstructure in ovariectomized rats. J Ethnopharmacol. 2012;142(1):80-85.
[23] Xu Y, Li D, Chen Q, et al. Full supervised learning for osteoporosis diagnosis using micro-CT images. Microsc Res Tech. 2013;76(4):333-341.
[24] 吴子祥,雷伟,胡蕴玉,等.骨质疏松绵羊模型松质骨及皮质骨的微观结构及力学性能变化的研究[J].中国骨质疏松杂志, 2007, 13(8):537-541.
[25] Yang J, Pham SM, Crabbe DL. High-resolution Micro-CT evaluation of mid- to long-term effects of estrogen deficiency on rat trabecular bone. Acad Radiol. 2003;10(10):1153-1158.
[26] Jiang Y, Zhao J, Liao EY, et al. Application of micro-CT assessment of 3-D bone microstructure in preclinical and clinical studies. J Bone Miner Metab. 2005;23:122-131.
[27] Frost HM. A 2003 update of bone physiology and Wolff's Law for clinicians. Angle Orthod. 2004;74(1):3-15.
[28] Chen H, Washimi Y, Kubo KY, et al. Gender-related changes in three-dimensional microstructure of trabecular bone at the human proximal tibia with aging. Histol Histopathol. 2011; 26(5):563-570.
[29] Thomsen JS, Niklassen AS, Ebbesen EN, et al. Age-related changes of vertical and horizontal lumbar vertebral trabecular 3D bone microstructure is different in women and men. Bone. 2013;57(1):47-55.
[30] Milovanovic P, Djonic D, Marshall RP, et al. Micro-structural basis for particular vulnerability of the superolateral neck trabecular bone in the postmenopausal women with hip fractures. Bone. 2012;50(1):63-68.
[31] Bevill G, Eswaran SK, Gupta A, et al. Influence of bone volume fraction and architecture on computed large-deformation failure mechanisms in human trabecular bone. Bone. 2006;39(6):218-225.
[32] Arlot ME, Burt-Pichat B, Roux JP, et al. Microarchitecture influences microdamage accumulation in human vertebral trabecular bone. J Bone Miner Res. 2008;23(10):1613-1618.
[33] Brouwers JE, van Rietbergen B, Huiskes R, et al. Effects of PTH treatment on tibial bone of ovariectomized rats assessed by in vivo micro-CT. Osteoporos Int. 2009;20(11):1823-1835.
[34] 李展春,程光齐,张自明,等.骨质疏松与骨关节炎患者松质骨的显微结构[J].国际病理科学与临床杂志,2011,31(1):17-20.