In vitro cytocompatibility of biomedical (Y0.95Eu0.05)2O3 nanomaterials

doi:10.3969/j.issn.2095-4344.2015.12.009

Abstract

Abstract:

BACKGROUND: Compared with organic fluorescent dyes and fluorescent proteins, rare earth nanomaterials have higher sensitivity, better optical stability, and lower cytotoxicity.

OBJECTIVE: To investigate the in vitro cytocompatibility of (Y_0.95Eu_0.05)₂O₃ rare earth nanomaterial and the effect of amine functionalization on the material cytocompatibility.

METHODS:(Y_0.95Eu_0.05)₂O₃ nanomaterials were synthesized using sol-gel method, and underwent amine functionalization. Amine-functionalized samples (10, 25, 50, 100, 200 mg/L) and non-amine-functionalized (Y_0.95Eu_0.05)₂O₃ rare earth nanomaterial suspensions were co-cultured with vascular smooth muscle cells of Sprague-Dawley rats for 3 days. Cell proliferation was detected using Cell Counting Kit-8 assay. 100 mg/L amine-functionalized samples and non-amine-functionalized (Y_0.95Eu_0.05)₂O₃ rare earth nanomaterial suspensions were co-cultured with L929 cells for 48 hours. Cell apoptosis was observed using fluorescence staining.

RESULTS AND CONCLUSION: With increased mass concentration of material suspension, survival rate of vascular smooth muscle cells was gradually reduced. At the mass concentration of 10, 25, 50 mg/L, cell survival rate was significantly higher in the amine-functionalized group than in the non-amine-functionalized group (P < 0.05). When the mass concentration of materials reached 200 mg/L, the cell survival rate decreased to 76% in the non-amine-functionalized group, but it was still above 80% in the amine-functionalized group, showing significant differences between the two groups (P < 0.05). The growth of L929 cells was good and the number of apoptotic cells was less in the amine-functionalized group. Cells were sparse and the number of cell apoptosis was more in the non-amine-functionalized group, showing slight cytotoxicity. These data verified that amine-functionalization can improve cytocompatibility of (Y_0.95Eu_0.05)₂O₃ rare earth nanomaterial.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Nanostructures, Materials Testing, Lanthanoid Series Elements

CLC Number:

Ring18

Shuai Meng-qi, Zhu Wen-lu, Wang You-fa. In vitro cytocompatibility of biomedical (Y_0.95Eu_0.05)₂O₃ nanomaterials[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(12): 1846-1851.

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References

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In vitro cytocompatibility of biomedical (Y_0.95Eu_0.05)₂O₃ nanomaterials

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