Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (34): 5501-5509.doi: 10.3969/j.issn.2095-4344.0686

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Preparation of silk fibroin/polycaprolactone temporary rotator cuff patch by electrospinning technique

Guo Ganggang1, 2, Pang Yabo3, Yang Jianhua1, Liu Shichen1, Gao Shuang4, Xiao Tongguang5, Zhang Weixiang6, Zhai Raosheng1,   Guo Quanyi2   

  1. 1Jiamusi University, Jiamusi 154007, Heilongjiang Province, China; 2Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, PLA Key Laboratory of Orthopaedic Trauma, Beijing 100853, China; 3Xuzhou Medical University, Xuzhou 221004, Jiangsu Province, China; 4Peking University, Beijing 100871, China; 5Haozhou People’s Hospital, Haozhou 236800, Anhui Province, China; 6Lanxi People’s Hospital, Lanxi 321100, Zhejiang Province, China
  • Received:2018-07-24 Online:2018-12-08 Published:2018-12-08
  • Contact: Guo Quanyi, Professor, Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, PLA Key Laboratory of Orthopaedic Trauma, Beijing 100853, China
  • Supported by:

    the National Nature Science Foundation of China, No. 81472092, 81772319; the National Key Research and Development Plan of China, No. 2017YFC1104102, 2017YFC1103404; Beijing Natural Science Foundation, No. 7172203; Beijing Science and Technology Special Project, No. Z161100005016059; the Key Science and Technology Project of Jiamusi University, No. 12Z1201507

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Abstract:

BACKGROUND: As the rate of re-fracture of the rotator is very high, the rotator cuff patch is designed to enhance the healing of the injured rotator cuff. Therefore, the preparation of temporary rotator cuff patch with good biocompatibility, biodegradability and biomechanical properties has become a committed step.

OBJECTIVE: To prepare a temporary rotator cuff patch with different ratio of silk fibroin (SF)/polycaprolactone (PCL) using electrospinning technique, and to evaluate its physiochemical properties and biocompatibility.
METHODS: Temporary rotator cuff patches with SF/PCL at different mass ratios of 4:0, 3:1, 1:1, 1:3, 0:4 were prepared using the electrospinning technique. Microstructure, contact angle, degradation properties and composition of the patches were characterized. Temporary rotator cuff patches with the mass ratios of 3:1, 1:1, 1:3, and 0:4 were implanted subcutaneously into the back of Sprague-Dawely rats. After 2 and 4 weeks of implantation, the patch material was taken for hematoxylin-eosin staining.
RESULTS AND CONCLUSION: (1) The temporary rotator cuff patches prepared from the mixed material improved the diameter, aperture number, and pore diameter of the pure PCL patch as well as the surface droplets caused by the high brittleness of the pure SF patch. (2) The temporary rotator cuff patches prepared by mixed materials improved hydrophilicity as compared with the SF patch and improved hydrophobicity as compared with the simple PCL patch. (3) In the temporary rotator cuff patch prepared by mixed materials, no chemical reaction occurred between the two materials. (4) With the exception of the temporary rotator cuff patch with a mass ratio of 0:4, the rest patches showed an increase in quality after immersed in PBS for 4 weeks. (5) Two weeks after subcutaneous implantation, a large number of cells were attached to the rotator cuff patch in each group, and capillaries formed in the neonatal tissues. The outer middle layer of the rotator cuff patch had a microstructure similar to the normal tendon tissue, while the middle layer of the pure PCL patch was the worst in microstructure. (6) Four weeks after subcutaneous implantation, the number of cells in the neonatal tissue of the patch significantly decreased, and the thickness of the outer neonatal tissue similar to the normal tendon tissue increased, while the pure PCL patch showed the worst function in the middle layer of the new tissue. To conclude, the results show that SF improves the potential of PCL patch to induce the cell differentiation into tendon cells.  

Key words: Fibrin, Materials Testing, Tissue Engineering

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