Biocompatibility of a novel calcium-magnesium phosphate composite cement in dentistry

doi:10.3969/j.issn.2095-4344.0013

Abstract

Abstract:

BACKGROUND: In our preliminary experiments, calcium phosphate cement, magnesium phosphate cement, calcium silicate and bismuth were mixed to manufacture a novel inorganic composite material, which would be used to repair furcal perforation. This novel composite cement has been confirmed to have excellent physiochemical and mechanical properties.

OBJECTIVE: To study the biocompatibility of calcium phosphate-magnesium phosphate-calcium silicate-bismuth cement (CMSBC).

METHODS: Cytotoxicity test: Relative growth rate of L929 cells cultured in CMSBC extract was analyzed using cell counting kit-8 assay. Acute hemolysis test: The leaching solution of test samples, saline and distilled water were added into the rabbit anti-clotting, to detect the rate of hemolysis. Acute systemic toxicity test: CMSBC lead liquor and normal saline were respectively injected via the tail vein into the mice in experimental group and control group, respectively. The general situation of mice was observed at 24, 48, 72 hours respectively and body weight change was valued. Ames test: In the flat-plate incorporation test of Salmonella typhimurium, the average number of spontaneous revertants of TA97, TA98, TA100 and TA102 of CMSBC leaching liquor was calculated after incubation at 37 ℃ for 72 hours. S-9 mixture was added to the plates as an in vitro activation system.

RESULTS AND CONCLUSION: The cytotoxicity of CMSBC in vitro was grade 0. Negative results from the acute hemolytic test, acute systemic toxicity test and Ames test of CMSBC indicate that this novel inorganic composite material has good biocompatibility and biosecurity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Calcium Phosphates, Biocompatible Materials, Materials Testing, Tissue Engineering

CLC Number:

R318

Shen Qing-yi, Li Guo-qiang, Li Kai.

Biocompatibility of a novel calcium-magnesium phosphate composite cement in dentistry

[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(2): 241-247.

Figures/Tables 4

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