X-ray evaluation of alpha-calcium sulfate hemiydrate/biphasic bioceramics bone for repairing bone defects of rabbit radius

doi:10.3969/j.issn.2095-4344.2014.47.002

Abstract

Abstract:

BACKGROUND: Alpha-calcium sulfate hemiydrate/biphasic bioceramics (α-CSH/BCP) as a bone repair material has been widely used in the clinic, but there are still some deficiencies.
OBJECTIVE: To observe the effect of α-CSH/BCP on the repair of bone defects of the radius in rabbits.
METHODS: The unilateral radius of 18 adult New Zealand white rabbits was selected to establish bone defect models, and then these rabbit models were randomized into three groups that were treated with α-CSH, α-CSH/BCP, BCP materials, respectively. At 4, 8, 12 weeks after implantation, bone samples were taken for gross and X-ray observations.
RESULTS AND CONCLUSION: (1) Gross observation: At 12 weeks of implantation, the bone defect was completely repaired in the α-CSH/BCP group, and the defected radius recovered basically; in the α-CSH group, bone defects were visible; and in the BCP group, there was still some material for filling and coverage. (2) X-ray observation: In the α-CSH group, the degradation of implanted materials was faster than new bone formation, and bone defects were visible; in the α-CSH/BCP group, bone defects were healed, cortical bone remodeling was good, and the bone marrow cavity was re-canalized; in the BCP group, the implanted materials were still visible, and bone defects were not repaired. These findings indicate that the α-CSH/BCP bone repair material can significantly promote the repair of bone defects of rabbit radius, with a better repair effect than α-CSH or BCP alone.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: calcium sulfate, ceramics, X-rays

CLC Number:

R318

Yao Biao, Qian Wei-qing, Yin Hong. X-ray evaluation of alpha-calcium sulfate hemiydrate/biphasic bioceramics bone for repairing bone defects of rabbit radius[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(47): 7550-7555.

Figures/Tables 3

References

[1] Nishida J, Shimamura T. Methods of reconstruction for bone defect after tumor excision: a review of alternatives. Med Sci Monit.2008;14(8): RA107-RA113.
[2] Al-Nawas B, Schiegnitz E.Augmentation procedures using bone substitute materials or autogenous bone-a systematic review and meta-analysis. Eur J Oral Implantol. 2013;7(2): 219-234.
[3] 朱美忠,李晓斌,陈滔.纳米羟基磷灰石/聚酰胺66复合生物活性人工骨在肢体骨缺损应用87例[J].创伤外科杂志, 2014,16(1):29-31.
[4] 许宋锋,于秀淳,徐明.多孔磷酸三钙修复良性骨肿瘤骨缺损的临床研究[J].生物骨科材料与临床研究,2011,8(3):10-13.
[5] 刘晓阳,李广润,刘洪涛,等.硫酸钙人工骨/骨髓间充质干细胞构建组织工程化骨诱导脊柱融合[J].中国组织工程研究, 2014, 18(21): 3281-3286.
[6] Nandi SK,Roy S,Mukherjee P,et al.Orthopaedic applications of bone graft & graft substitutes: A review.Indian J Med Res. 2010;132(7):15-30.
[7] Thomas MV,Puleo DA.Calcium sulfate: Properties and clinical applications.J Biomed Mater Res B Appl Biomater. 2009; 88(2):597-610.
[8] Hu G, Xiao L, Fu H, et al. Study on injectable and degradable cement of calcium sulphate and calcium phosphate for bone repair.J Mater Sci Mater Med.2010;21(2):627-634.
[9] Hao Q, Zhao L, Guan JK, et al. Application of artificial bone materials to repair bone defects. J Clin Rehabil Tissue Eng Res.2009;13(34):6745-6748.
[10] Jensen SS, Bornstein MM, Dard M, et al. Comparative study of biphasic calcium phosphates with different HA/TCP ratios in mandibular bone defects. A long-term histomorphometric study in minipigs.J Biomed Mater Res B Appl Biomater. 2009; 90(1):171-181.
[11] 谭迎赟,白石,廖运茂.双相钙磷生物陶瓷/硫酸钙骨水泥多孔三维支架的生物性能[J].中国组织工程研究,2014,18(8):1161-1164.
[12] Hu G, Xiao L, Fu H, et al. Study on injectable and degradable cement of calcium sulphate and calcium phosphate for bone repair.J Mater Sci Mater Med. 2010;21(2):627-634.
[13] Nandi SK,Roy S,Mukherjee P,et al.Orthopaedic applications of bone graft & graft substitutes: A review.Indian J Med Res. 2010;132(7):15-30.
[14] Wagner W,Wiltfang J,Pistner H,et al.Bone formation with a biphasic calcium phosphate combined with fibrin sealant in maxillary sinus floor elevation for delayed dental implant. Clin Oral Implants Res.2012;23(9):1112-1117.
[15] 章庆国,赵士芳,郭宗科,等.纳米相陶瓷支架与人成骨细胞生物相容性的体外实验研究[J].东南大学学报:自然科学版,2004,34(2): 219-223.
[16] 钱卫庆,王宸,陈昌红.BCP/HAFG—rhBMP-2复合人工骨修复骨缺损的实验研究[J].现代医学,2007,35(4):265-270.
[17] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. Beijing: 2006:30.
[18] Chen G,Ushida T,Tateishi T. Hybrid biomaterials for tissue engineering: A preparative method for PLA or PLGA-collagen hybrid sponges.Adv Mater.2000;12(6):455-457.
[19] Alves Cardoso D,Jansen JA,Leeuwenburgh SC. Synthesis and application of nanostructured calcium phosphate ceramics for bone regeneration.J Biomed Mater Res B Appl Biomater.2012;100(8): 2316-2326.
[20] De Long WG Jr,Einhorn TA,Koval K,et al. Bone Grafts and Bone Graft Substitutes in Orthopaedic Trauma Surgery.J Bone Joint Surg Am.2007;89(3):649-658.
[21] Liu Y,Lim J,Teoh SH.Review: Development of clinically relevant scaffolds for vascularised bone tissue engineering. Biotechnol Adv.2013;31(5):688-705.
[22] Pettier LF,Jones RH.Treatment of unicameral bone cysts by curettage and packing with plaster of paris pellets.J Bone Joint Surg.1978; 60( 6): 820-822.
[23] Xu HHK,Quinn JB,Takagi S,et al.Synergistic reinforcement of in situ hardening calcium phosphate composite scaffold for bone tissue engineering. Biomaterials.2004;25:1029-1037.
[24] Thomas MV,Puleo DA.Calcium sulfate: properties and clinical applications. J Biomed Mater Res Part B ApplBiomater. 2009; 88(2):597-610.
[25] Robert N,Steensen MD,Ryan M,et al.A simple teclunique for reconstrction of the medial patellofenoral ligament using a quadriceps tendon graft. Arthroscopy.2005;210:365-370.
[26] Walsh WR,Morberg P,Yu Y,et al.Response of a calcium sulphate bone graft substitute in a confined cancellous defect.Clin orthop Relat Res.2003;406:228-236.
[27] Nilsson M,Wang JS,Wielanek L,et al.Biodegradation and biocompat -ibility of a calcium sulphate-hydroxyapatite bone substitute.J Bone Joint Surg Br.2004;86:120-125.
[28] Hing KA,Wilson LF,Buckland T.Comparative performance of three ceramic bone graft substitutes.Spine J.2007;7:475-490.
[29] Bodde EW,Wolke JG,Kowalski RS,et al.Bone regeneration of porous β-tricalcium phosphate (Conduit™ TCP) and of biphasic calcium phosphate ceramic (Biosel®) in trabecular defects in sheep. J Biomed Mater Res A.2007; 82(3): 711-722.
[30] 陈雪宁.羟基磷灰石陶瓷结构对细胞成骨诱导作用的研究[D].成都:四川大学,2008.
[31] Habibovic P,de Groot D. Osteoinductive biomaterials-- properties and relevance in bone repair.J Tissue Eng Regen Med.2007;1(1):25-32.
[32] Ramay H,Zhang M. Biphasic calcium phosphate nanocomposite porous scaffolds for load-bearing. bone tissue engineering. Biomaterials. 2004;25:5171-5180.
[33] Roldon JC, Detsch R, Schaefer S, et al.Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model. J Craniomaxillofac Surg.2010;38(6):423-430.
[34] Lazáry A,Balla B,Kósa J,et al.Review of the application of synthetic bone grafts. The role of the gypsum in bone substitution: molecular biological approach, based on own research results.Orvosi Hetilap.2008;148(51):2427-2433.
[35] 王宏,丁洁涛,朱向东,等.不同孔隙率的双相磷酸钙陶瓷降解性能研究[J].功能材料, 2010,41(10):1727-1730.