Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (6): 829-835.doi: 10.3969/j.issn.2095-4344.2017.06.002
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Received:
2016-12-02
Online:
2017-02-28
Published:
2017-03-16
Contact:
Zeng Jian-cheng, Associate professor, Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China
About author:
Wang Xian-di, Master, Physician, Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China
Supported by:
the Science and Technology Program of the Science & Technology Department of Sichuan Province, No. 2012SZ0128
CLC Number:
Wang Xian-di, Chen Guo, Li Zhu-hai, Jiang Hu-shan, Nie Hong-fei, Xie Tian-hang, Huang Hong-jie, Pei Fu-xing, Song Yue-ming, Zeng Jian-cheng. Calcium sulfate bone cement with or without injectable polyamino acid for goat vertebral defects: a comparative experiment[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(6): 829-835.
2.1 一般情况 所有实验动物均进入结果分析。切口均Ⅰ/甲愈合,无感染、无神经脊髓损伤。取材时见外口骨蜡存在,骨蜡覆盖处有不同量新骨形成。 2.2 Mirco-CT横断面观察 两组在4个不同时间点的Micro-CT二维扫描图像见图1,2。 4周末时,两组骨缺损内均未见填充材料。在骨缺损边缘均有少量骨小梁生成,PAA/CS组底部生成的骨小梁较硫酸钙组多。 8周末时,两组骨缺损有缩小趋势,硫酸钙组骨缺损底部有一定新骨生成。PAA/CS组除在底部有新骨生成外,在骨缺损的外口部也有少量的新骨生成,骨缺损已变得不规则。新生骨小梁密度稍高于周围正常骨小梁,排列结构欠清晰。 12周末时,硫酸钙组新生骨较多,骨缺损已变得不规则,底部和中部新生骨小梁已有少部分相互链接。PAA/CS组新生骨小梁明显,在骨缺损外口处和中间部相连,将其分隔。 16周末时,硫酸钙组骨缺损尚剩余不规则腔隙,新生骨小梁密度较高,排列欠规则。PAA/CS组新生骨小梁几乎将骨缺损完全充填,骨小梁结构清晰,连接良好。在Micro-CT二维横断面上显示骨缺损残留硫酸钙组多于PAA/CS组。"
2.4 硬组织切片观察结果 术后4周(图3):两组标本内可见到清晰的骨缺损,内填充成纤维细胞,有淋巴细胞灶状浸润。骨缺损内有较多胶原纤维生成,周边部可见到有少量新生骨小梁,有成骨细胞整齐排列于表面,可见较多炎性细胞浸润,炎性细胞在PAA/CS组标本中明显少于硫酸钙组。 术后8周(图4):两组标本骨缺损中的新生骨小梁数目均有增多,可见排列整齐的成骨细胞围绕新生骨小梁排列。硫酸钙组新生骨小梁主要集中在骨缺损底部,PAA/CS组标本在底部及中间部均有新生骨小梁生成。PAA/CS组骨缺损中心部位仍可见淋巴细胞浸润。 术后12周(图5):两组骨小梁数目继续增多、增粗、密度增高。周边部分编织骨已开始转化为板层骨,具有正常骨小梁的形态,靠近缺损中心的骨小梁周围仍然可见成骨细胞规则排列在骨缺损底部,骨小梁形成于中间部分。PAA/CS组中间部分骨小梁形成多于硫酸钙组。 术后16周(图6):两组均有大量骨小梁生成,部分连接,大部分新生骨小梁已具有正常骨小梁结构。"
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