Endothelial progenitor cells repair ischemia-reperfusion injury in rats

doi:10.3969/j.issn.2095-4344.2014.32.011

Abstract

Abstract:

BACKGROUND: Endothelial progenitor cells are recruited into local vascular injury under the injury-induced stimulation, and then differentiate into mature endothelial cells that are thereby involved in angiogenesis and endothelial repair.
OBJECTIVE: To investigate whether endothelial progenitor cells can alleviate renal injury and improve renal function of ischemia/reperfusion injury (I/R) rats.
METHODS: Peripheral blood samples extracted from Sprague-Dawley rats were used to isolate and culture endothelial progenitor cells using density gradient centrifugation. Twenty-two male Sprague-Dawley rats were randomized to three groups: I/R group, normal control group, and endothelial progenitor cells group. In the I/R and endothelial progenitor cells groups, the right kidney was removed and the renal artery and vein of the left kidney were occluded for 40 minutes to establish I/R models in the rats, and then endothelial progenitor cells (5×109/L, totally 1 mL) or solvent was transplanted via the artery of the left kidney into the left kidney. In the normal control group, the experimental procedure was same as that in the I/R group except for occlusion of the
artery and vein of the left kidney. Renal and blood samples from three groups were collected at day 1 after operation. Peripheral blood CD34 and vascular endoethelial growth factor receptor 2 expressions were determined using flow cytometry and immunofluorescence methods, serum creatinine and urea nitrogen were tested, and immunohistochemistry observation was used for CD34 observation.
RESULTS AND CONCLUSION: Compared with the normal control group, serum creatinine and urea nitrogen levels were significantly increased, and tubulointerstitial CD34 expression was decreased in the I/R group (P < 0.05). Endothelial progenitor cells treatment largely decreased the levels of serum creatinine and urea nitrogen, and increased CD34 expression (P < 0.05). These findings indicate that transplantation of endothelial progenitor cells contributes to renal protection in I/R rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: endothelial cells, reperfusion injury, kidney, blood urea nitrogen

CLC Number:

R394.2

Zhou Li-na, Wang Yu-xin, Fang Lin, Wu Ting, Yu Yi. Endothelial progenitor cells repair ischemia-reperfusion injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(32): 5146-5151.

Figures/Tables 3

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