Collagen/bioactive glass/chitosan composite scaffolds

doi:10.3969/j.issn.2095-4344.2014.21.016

Abstract

Abstract:

BACKGROUND: Collagen/bioactive glass composite materials possess excellent osteogenic potential and biocompatibility, but its application in bone tissue engineering is limited by mechanical property and degradation.
OBJECTIVE: To construct collagen/bioactive glass/chitosan composite scaffolds with good mechanical property, anti-degradation ability and bone repair property.
METHODS: Bioactive glass/collagen composite scaffolds with chitosan as dispersant were prepared by lyophylization. Fourier transform infrared spectroscopy, scanning electron microscope, X-ray diffraction, and dynamic biomechanical testing were used to characterize the structure and properties of the composite scaffolds.
RESULTS AND CONCLUSION: Results show that charge-attractions in pre-prepared bioactive glass/chitosan solution increased the homogeneity of bioactive glass dispersed in collagen gel and the compressive modulus and strength increased significantly due to the homogeneity and intermolecular interactions between chitosan and collagen. The enzymatic degradation rate and mineralization activity in the simulated body fluid were also lower because of a high degree of embedment of bioactive glass in collagen/chitosan matrix, and entanglement of collagen in chitosan at molecular level, which decreased the exposure of bioactive glass to the simulated body fluid, and collagen to enzyme solution.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, chitosan, collagen, glass

CLC Number:

R318

Meng Yong-chun, Chen Xiao-feng, Nan Kai-hui, Li Yu-li, Luo Xiao-gang, Deng Chun-lin. Collagen/bioactive glass/chitosan composite scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3367-3373.

Figures/Tables 6

2.2 复合支架材料分子间的相互作用分析图2为支架中不同组分的红外图谱。由胶原红外图谱中，胶原蛋白的酰胺A谱带(3 325- 3 330 cm-1)主要是由N-H的伸缩振动引起的，酰胺A谱带对胶原蛋白三螺旋结构很敏感；酰胺Ⅰ谱带(1 650 cm-1)通常不受肽链侧基影响，振动频率取决于肽链构型，由蛋白多肽骨架的C=O伸缩振动引起的，为蛋白质的二级结构变化的敏感区域，且其吸收最强，常被用于蛋白质的二级结构分析，同时对三螺旋结构的变化也非常敏感[21]；酰胺Ⅱ谱带(1 554 cm-1)主要反映胶原蛋白的C-N(具有双键性质)伸缩振动和面内N-H弯曲振动，同时也是α-螺旋、β-折叠、转角和无规卷曲叠加的吸收带。与酰胺Ⅰ谱带相同，酰胺Ⅱ谱带也不易受肽链侧基影响，但对胶原蛋白的三螺旋结构不敏感[22]；酰胺Ⅲ谱(1 240 cm-1)带主要由同相N-H弯曲振动和C-N伸缩振动共同引起的，此外还含些许C-O面内弯曲振动和C-C伸缩振动。此谱带的振动组成比较复杂，侧链结构对其有不同程度的影响，但此谱带仍可用于蛋白质二级结构预测[23]。酰胺Ⅰ带只含C=O伸展振动，而在Ⅱ，Ⅲ带中，每一种酰胺带都包含两种振动方式，而且都含氨基N-H键的面内弯曲振动。与Ⅰ带峰的强度相比，Ⅱ、Ⅲ带峰的强度显著降低，表明N-H键面内弯曲振动受阻的程度远大于C=O键的伸展振动。从壳聚糖曲线知，其特征谱带在3 433，2 917，2 877，1 599，1 422 cm-1分别代表着-OH，-CH2，-CH，N-H弯曲振动，伯碳原子醇羟基-OH振动峰。在1 646处为C=O很弱的伸缩振动峰，表明壳聚糖的脱乙酰化程度很高(≥92%)[24]。从生物活性玻璃曲线可知，其特征峰1 071，799，470 cm-1分别代表着Si-O-Si非对称伸缩振动峰、Si-O-Si的对称伸缩振动峰、Si-O-Si的弯曲振动峰[25-26]。"

References

[1]Kushitani S,Yasukawa E,Iwaki H,et al.Particulate bioglass compared with hydroxyapatite as a bone graft substitute.Clin Orthop Relat Res. 1997;(334):316-325.
[2]Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21:2529-2543.
[3]Li HY,Du RL,Chang J.Fabrication,characterization, and in vitro degradation of composite scaffolds based on PHBV and bioactive glass.J Biomater Appl. 2005;20:137-155.
[4]Shalumon KT,Sowmya S,Sathish D,et al.Effect of Incorporation of Nanoscale Bioactive Glass and Hydroxyapatite in PCL/Chitosan Nanofibers for Bone and Periodontal Tissue Engineering.J Biomed Nanotechnol. 2013; 9:430-440.
[5]Tamjid E,Bagheri R,Vossoughi M,et al.Effect of particle size on the in vitro bioactivity, hydrophilicity and mechanical properties of bioactive glass-reinforced polycaprolactone composites.Mat Sci Eng C-Mater.2011;31:1526-1533.
[6]Alm JJ,Frantzen JPA,Moritz N,et al.In vivo testing of a biodegradable woven fabric made of bioactive glass fibers and PLGA(80)-A pilot study in the rabbit.J Biomed Mater Res B.2010;93B:573-580.
[7]Orava E,Korventausta J,Rosenberg M,et al.In vitro degradation of porous poly(DL-lactide-co-glycolide) (PLGA)/bioactive glass composite foams with a polar structure. Polym Degrad Stabil.2007;92:14-23.
[8]Yang CR,Wang YJ,Chen XF.Mineralization regulation and biological influence of bioactive glass-collagen- phosphatidylserine composite scaffolds.Sci China Life Sci. 2012;55:236-240.
[9]Han X,Chen XF,Meng YC,et al.Biocompatibility of the Composite Scaffold of Sol-Gel Bioactive Glass/Collagen.J Inorg Mater.2011;26:869-873.
[10]Leu A,Leach JK.Proangiogenic potential of a collagen/bioactive glass substrate. Pharm Res.2008;25:1222-1229.
[11]Luz GM,Mano JF.Chitosan/bioactive glass nanoparticles composites for biomedical applications.Biomed Mater. 2012; 7:054104.
[12]Couto DS,Hong ZK,Mano JF.Development of bioactive and biodegradable chitosan-based injectable systems containing bioactive glass nanoparticles.Acta Biomater.2009;5:115-123.
[13]Bhowmik R,Katti KS,Katti DR.Mechanics of molecular collagen is influenced by hydroxyapatite in natural bone.J Mater Sci.2007;42:8795-8803.
[14]Verschueren DS,Gassner R,Mitchell R,et al.The effects of guided tissue regeneration (GTR) on modified Le Fort I osteotomy healing in rabbits.Int J Oral Max Surg.2005;34: 650-655.
[15]Yunoki S,Ikoma T,Tanaka J.Development of collagen condensation method to improve mechanical strength of tissue engineering scaffolds.Mater Charact.2010;61:907-911.
[16]Zhang LH,Tang PF,Zhang W,et al.Effect of Chitosan as a Dispersant on Collagen-Hydroxyapatite Composite Matrices. Tissue Eng Part C-Me.2010;16:71-79.
[17]Richert L,Lavalle P,Payan E,et al.Layer by layer buildup of polysaccharide films: physical chemistry and cellular adhesion aspects.Langmuir.2003;20:448-458.
[18]Yin YJ,Yao KD,Cheng GX,et al.Properties of polyelectrolyte complex films of chitosan and gelatin.Polym Int.1999;48: 429-432.
[19]Chen XF,Meng YC,Li YL,et al.Investigation on bio-mineralization of melt and sol-gel derived bioactive glasses. Appl Surf Sci.2008;255:562-564.
[20]Nam K,Kimura T,Kishida A.Preparation and characterization of cross-linked collagen-phospholipid polymer hybrid gels. Biomaterials.2007;28:1-8.
[21]Payne KJ,Veis A.Fourier transform ir spectroscopy of collagen and gelatin solutions: Deconvolution of the amide I band for conformational studies. Biopolymers. 1988; 27: 1749-1760.
[22]Doyle BB,Bendit EG,Blout ER.Infrared spectroscopy of collagen and collagen-like polypeptides.Biopolymers. 1975; 14:937-957.
[23]Barth A.Infrared spectroscopy of proteins.Biochimica et Biophysica Acta. 2007;1767:1073-101.
[24]Shanmugasundaram N,Ravichandran P,Reddy PN,et al.Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells. Biomaterials. 2001;22:1943-1951.
[25]Costa HS,Rocha MF,Andrade GI,et al.Sol-gel derived composite from bioactive glass-polyvinyl alcohol.J Mater Sci.2008;43:494-502.
[26]Mansur HS,Oréfice RL,Mansur AAP.Characterization of poly(vinyl alcohol)/poly(ethylene glycol) hydrogels and PVA-derived hybrids by small-angle X-ray scattering and FTIR spectroscopy.Polymer.2004;45:7193-7202.
[27]Kida T,Nagasaka Y,Sakurai T, et al.Growth and Characterization of SiC Films by Hot-Wire Chemical Vapor Deposition at Low Substrate Temperature Using SiF4/CH4/H2 Mixture.Jpn J Appl Phys.2008;47:566-568.
[28]Rhee SH,Tanaka J.Hydroxyapatite coating on a collagen membrane by a biomimetic method.J Am Ceram Soc.1998; 81:3029-3031.
[29]Rhee SH,Lee JD,Tanaka J.Nucleation of hydroxyapatite crystal through chemical interaction with collagen.J Am Ceram Soc.2000;83:2890-2892.
[30]Sionkowka A,Wisniewski M,Skopinska J,et al. Molecular interactions in collagen and chitosan blends.Biomaterials.2004;25:795-801.
[31]Taravel MN,Domard A.Collagen and its interactions with chitosan : III. Some biological and mechanical properties. Biomaterials.1996;17:451-455.
[32]孟永春.胶原与生物活性玻璃作用机理及其复合骨修复材料的研究[D].广州:华南理工大学博士学位论文,2010.
[33]Wang K,Li WW,Gao C.Poly(epsilon-caprolactone)- functionalized carbon nanofibers by surface-initiated ring-opening polymerization.J Appl Polym Sci.2007;105: 629-640.