Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (20): 3133-3138.doi: 10.3969/j.issn.2095-4344.2014.20.004

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Survival of engineered myocardium constructed by bone marrow mesenchymal stem cells in vitro

Tian Mao1, Piao Hai-nan2, Chen Yu3, Zhao Qi4, Pan Xin5   

  1. 1Luzhou People’s Hospital, Luzhou 646010, Sichuan Province, China; 2Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China; 3The First People’s Hospital of Ziyang, Ziyang 641300, Sichuan Province, China; 4Zunyi Medical College, Zunyi 563000, Guizhou Province, China; 5Dalian Central Hospital Affiliated to Dalian Medical University, Dalian 116033, Liaoning Province, China
  • Received:2014-03-12 Online:2014-05-14 Published:2014-05-14
  • Contact: Piao Hai-nan, Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China
  • About author:Tian Mao, Luzhou People’s Hospital, Luzhou 646010, Sichuan Province, China
  • Supported by:

    the Dalian Municipal Science and Technology Bureau Project, No. 2006E21SF092

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Abstract:

BACKGROUND: So far, engineered myocardium is still facing many problems. Research has demonstrated that bone marrow mesenchymal stem cells can be differentiated into myocardial cells. Polyglycolide and polycaprolactone are commonly used artificial polymers, which have good biocompatibility.
OBJECTIVE: To observe the growth of the poly(glycolic acid)/poly(e-caprolactone) copolymer patch in vitro in normal myocardium and infarcted myocardium.
METHODS: Bone marrow mesenchymal stem cells in Sprague-Dawley rats were separated using adherent separation and selection method, cultured in vitro. The third passage was labeled with DAPI. The bone marrow mesenchymal stem cell suspensions (2×106/cm2) were produced and planted on poly(glycolic acid)/ poly(e-caprolactone) copolymer scaffolds to form poly(glycolic acid)/poly(e-caprolactone) copolymer patch. After culturing for 48 hours, the specimens were observed under electron microscope, stained with hematoxylin and eosin, and then observed under light microscope. Rat models of myocardial infarction were established by ligating left anterior descending coronary artery. Poly(glycolic acid)/poly(e-caprolactone) copolymer patch was implanted into the normal and infarcted myocardium for 5 weeks. The survival of bone marrow mesenchymal stem cells was determined by the detection of pathology.
RESULTS AND CONCLUSION: Results of light microscope and electron microscope demonstrated that bone marrow mesenchymal stem cells grew three-dimensionally on poly(glycolic acid)/poly(e-caprolactone) copolymer patch. Cells and patch were adhesive well. Under laser confocal microscopy, compared with the first week, bone marrow mesenchymal stem cells were marked by DAPI in the myocardium at the fifth week. There were bone marrow mesenchymal stem cells marked by DAPI in the infracted area. Results of hematoxylin-eosin staining exhibited that bone marrow mesenchymal stem cells were detected in the infarct area. These results suggested that bone marrow mesenchymal stem cells adhered to the poly(glycolic acid)/poly(e-caprolactone) copolymer stent well. The complexes of poly(glycolic acid)/poly(e-caprolactone) copolymer and bone marrow mesenchymal stem cells can be used for reparation of myocardium.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: stem cells, mesenchymal stem cells, myocardial infarction, stents, staining and labeling

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