The stem/progenitor cell characteristics of trabecular meshwork endothelia cells in the adult human eye

doi:10.3969/j.issn.2095-4344.2014.14.013

Abstract

Abstract:

BACKGROUND: The study of stem/progenitor cells provides the possibility of stem cell therapy, and identification and characterization of stem/progenitor cells in human trabecular meshwork can put new thoughts on treatment of primary open-angle glaucoma.
OBJECTIVE: To study the characteristics of stem/progenitor cells in trabecular meshwork in the adult human eye.
METHODS: Hematoxylin and eosin staining and immunohistochemistry staining of nestin, Factor VIII-related antigen, CD31 and CD34 were applied in 20 enucleated adult human eyes were selected, among which, 10 normal eyes had enucleation due to sudden death for serious trauma, 7 eyes were from the patients who had orbital exenteration, and 3 eyes were diagnosed as posterior segment choroidal malignant melanoma.
RESULTS AND CONCLUSION: The trabecular meshwork endothelial cells in the human eye were all positive for nestin, but negative for the other three anti-bodies. The corneal endothelial cells were weakly positive for nestin. Schlemm’s canal endothelial cells were negative for nestin, but strongly positive for the Factor VIII-related antigen, CD31 and CD34. These findings indicate that the trabecular meshwork endothelial cells in the human eye all express nestin with stem/progenitor cell characteristics, and they are similar to the vascular progenitor cells with nestin expression.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, eye, endothelial cells, trabecular meshwork

CLC Number:

R394.2

Wan Shang-tao, Li Yong-ping, Zhang Ping, Gao Huan-huan, Zhang Wen-xin, Lin Jian-xian. The stem/progenitor cell characteristics of trabecular meshwork endothelia cells in the adult human eye[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(14): 2213-2218.

Figures/Tables 1

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