Effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging mice

doi:10.3969/j.issn.2095-4344.2014.14.012

Abstract

Abstract:

BACKGROUND: Both human umbilical cord mesenchymal stem cells and lycopene have the anti-aging actions. But the research about their combination effects on immune function of aging body has not been reported.
OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging mice
METHODS: ICR mice, female and male in half, were randomly divided into five groups: normal control group, model group, lycopene group, stem cell group, stem cell combined with lycopene group (combined group). Model group and treatment groups were injected subcutaneously with D-galactose at the neck for 8 weeks. Age-related percentages of T cell subsets in the blood, contents of interleukin-2 and interleukin-4 in the serum were detected, and structure changes of spleen and thymus were observed.
RESULTS AND CONCLUSION: In three treatment groups, the percentages of CD3+, CD8+CD28+ T cells in the blood and contents of interleukin-2 and interleukin-4 in the serum were all improved (P < 0.01). In the combined
group, naive CD8+ T cells percentage increased, memory CD8+ T cells percentage decreased (P < 0.05), however in the stem cell and lycopene groups, naïve and memory CD8+ T cell percentages did not change significantly (P > 0.05). Naïve and memory CD4+ T cell percentages between groups did not change significantly (P > 0.05). Microscopic observations of the thymus and spleen showed that in the model group, thymic corpuscles shrunk, splenic nodules significantly increased, splenic medulla was blooded; while no obvious cortical and medullary lesions were observed in the normal control group and three treatment groups. The results showed that both human umbilical cord mesenchymal stem cells and lycopene can ameliorate the immune function of aging model mice, but the effect of combined treatment is better.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, umbilical cord, galactose, aging, immune tolerance, spleen, thymus gland

CLC Number:

R394.2

Liu Jia, Wang Yi-zhong, Cui Xiao-lan, Shi Han, Li Qian-qian, Wang Lin-lin. Effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging mice[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(14): 2206-2212.

Figures/Tables 5

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